A simple method for extracting C-phycocyanin from Spirulina platensis using Klebsiella pneumoniae

C-phycocyanin (C-PC) was extracted from fresh Spirulina platensis by deploying a species of non-pathogenic nitrogen-fixing bacteria, namely, Klebsiella pneumoniae. The algal slurry was neither washed nor centrifuged; the bacterial culture was poured into the slurry, the vessel sealed, and crude C-PC extracted after about 24 h. The extraction was clean and efficient, and the purity and concentration of C-PC proved to be of adequate quality.

[1]  M. Wyman.  An in vivo method for the estimation of phycoerythrin concentrations in marine cyanobacteria (Synechoccus spp.) , 1992 .

[2]  R. Gottlieb,et al.  Nitrogen cavitation for cell disruption to obtain mitochondria from cultured cells. , 2000, Methods in enzymology.

[3]  E. H. Harris.  1 – An Overview of the Genus Chlamydomonas , 1989 .

[4]  E. Talavera,et al.  Chromatographic purification of biliproteins from Spirulina platensis high-performance liquid chromatographic separation of their α and β subunits , 1997 .

[5]  H. Scheer,et al.  Conformational Studies on C-Phycocyanin from Spirulina platensis , 1977 .

[6]  F. H. Farmer,et al.  Extraction, identification, and quantitation of phycobiliprotein pigments from phototrophic plankton , 1984 .

[7]  K. Minkova,et al.  Purification of phycobiliproteins from Nostoc sp. by aminohexyl–Sepharose chromatography , 1999 .

[8]  S. Maeda,et al.  Rapid and selective extraction of phycocyanin from Spirulina platensis with ultrasonic cell disruption , 2003, Journal of Applied Phycology.

[9]  O. Pulz,et al.  Valuable products from biotechnology of microalgae , 2004, Applied Microbiology and Biotechnology.

[10]  Alejandro Cifuentes,et al.  Pressurized liquid extracts from Spirulina platensis microalga. Determination of their antioxidant activity and preliminary analysis by micellar electrokinetic chromatography. , 2004, Journal of chromatography. A.

[11]  C. Barwell,et al.  Purification and characterization of phycocyanin from the marine cyanobacterium Synechococcus sp. IO9201 , 1998 .

[12]  A. Richmond,et al.  Isolation and characterization of phycocyanins from the blue-green alga Spirulina platensis , 1979, Archives of Microbiology.

[13]  Gokare A. Ravishankar,et al.  Phycocyanin from Spirulina sp: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin , 1999 .

[14]  Sandhya Mishra,et al.  Purification and characterization of C-Phycocyanin from cyanobacterial species of marine and freshwater habitat. , 2005, Protein expression and purification.

[15]  M. Tchorbadjieva,et al.  Purification of C-phycocyanin from Spirulina (Arthrospira) fusiformis. , 2003, Journal of biotechnology.

[16]  E. H. Harris.  The Chlamydomonas sourcebook , 2009 .

[17]  A. Vonshak,et al.  Photoinhibition in outdoor Spirulina platensis cultures assessed by polyphasic chlorophyll fluorescence transients , 1999, Journal of Applied Phycology.

[18]  K. Kilpatrick,et al.  The development of a method to measure marine cyanobacterial phycoerythrin extracted in solvents , 1985 .

[19]  Y. Igarashi,et al.  Identification and characterization of phycobiliprotein from a Thermophilic cyanobacterium, Chroococcidiopsis sp. strain TS-821 , 1997 .

[20]  Pertti J Viskari,et al.  Rapid extraction of phycobiliproteins from cultured cyanobacteria samples. , 2003, Analytical biochemistry.