Anti-oxidant and Anti-inflammatory Effects of Hot Aqueous Extract on Lipopolysaccharide(LPS) Simulated Macrophages

Objectives : This study is to investigate the effects of Acanthopanacis Cortex hot aqueous extract on nitric oxide(NO), prostaglandin E2(PGE2) production and DPPH(1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity in macrophages. Methods : Acanthopanacis Cortex(200 g) was heated at 100 °C with distilled water(2 L) for 4hrs. The extract was filtered and concentrated to 100 ml using a rotary evaporator and was frozen at -80 °C, then was freeze-dried. The RAW 264.7 macrophages were subcultured. In order to evaluate cytotoxicity, MTT assay was performed. Experimental groups were divided into five(control, AC 25, 50, 100 and 200 μg/ml) and we measured cytotoxicity. The concentrations of NO were preprocessed by Griess assay. The RAW 264.7 macrophages was pretreated by 10 μg/ml LPS and experimental groups were divided into five and we measured NO production. The concentrations of PGE2 were measured by enzyme immunoassay. The RAW 264.7 macrophages was pretreated by 10 μg/ml LPS. Experimental groups were divided into five and we measured PGE2 production. Antioxidant activity was measured by the DPPH method. experimental groups were divided into four(AC 25, 50, 100 and 200 μg/ml) and we measured DPPH radical scavenging activity. Results : 1. Viability of RAW 264.7 macrophages did not significantly decrease in 25, 50 and 100 μg/ml Acanthopanacis Cortex hot aqueous extract compared to control group. 2. NO production in LPS-stimulated RAW 264.7 macrophages significantly inhibited in 100, 200 μg/ml Acanthopanacis Cortex hot aqueous extract compared to control group. 3. PGE2 production in LPS-stimulated RAW 264.7 macrophages significantly inhibited in 100, 200 μg/ml Acanthopanacis Cortex hot aqueous extract compared to control group. 4. DPPH radical scavenging capability of Acanthopanacis Cortex hot aqueous extract in RAW 264.7 macrophages had the high level in 100, 200 μg/ml. Conclusion : According to the results, Acanthopanacis Cortexx hot aqueous extract has ability to suppress NO, PGE2 production and improve DPPH free radical scavenging activity. So Acanthopanacis Cortex hot aqueous extract may have an anti-inflammation effect and antioxidant activity.