Effect of surface roughness on proliferation and alkaline phosphatase expression of rat calvarial cells cultured on polystyrene.

Rough-surfaced substrates made by a variety of methods have been shown to influence osteoblast proliferation and differentiation. The purpose of this study is to confirm the role of surface roughness in promoting osteoblastic differentiation using tissue culture polystyrene as substrate, by excluding factors other than roughness. Immature osteogenic cells derived from fetal rat calvariae were cultured on the plastic cover strips having varied degrees of roughness created by treatment with four kinds of grinding paper of different particle sizes. The proliferation and gene expression of alkaline phosphatase (ALP) and osteocalcin of the calvarial cells increased on the rough-surfaced cover strips. These levels increased in response to the increase in the degree of surface roughness up to 0.8 microm of average roughness and then decreased to the level observed for the smooth surface. These results demonstrate that the surface roughness itself caused increases in osteoblastic proliferation and differentiation in cell cultures.

[1]  S. Nomura,et al.  In situ hybridization of bone matrix proteins in undecalcified adult rat bone sections. , 1992, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[2]  R. Franceschi,et al.  Effects of ascorbic acid on collagen matrix formation and osteoblast differentiation in murine MC3T3‐E1 cells , 1994, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[3]  G. Stein,et al.  Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix , 1990, Journal of cellular physiology.

[4]  K. Hitomi,et al.  DEMONSTRATION OF ALKALINE PHOSPHATASE PARTICIPATION IN THE MINERALIZATION OF OSTEOBLASTS BY ANTISENSE RNA APPROACH , 1996, Cell biology international.

[5]  C. Devlin,et al.  Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures , 1991, Journal of cellular physiology.

[6]  S. Aota,et al.  Fibronectin regulates calvarial osteoblast differentiation. , 1996, Journal of cell science.

[7]  T. Kojo,et al.  Disaggregated osteoclasts increase in resorption activity in response to roughness of bone surface. , 1999, Journal of biomedical materials research.

[8]  N. Tsukagoshi,et al.  Increase in the activity of alkaline phosphatase by L-ascorbic acid 2-phosphate in a human osteoblast cell line, HuO-3N1. , 1992, Journal of nutritional science and vitaminology.

[9]  B. Boyan,et al.  Titanium surface roughness alters responsiveness of MG63 osteoblast‐like cells to 1α,25‐(OH)2D3 , 1998 .

[10]  B. Hogan,et al.  Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization , 1988, The Journal of cell biology.

[11]  L. Quarles,et al.  Distinct proliferative and differentiated stages of murine MC3T3‐E1 cells in culture: An in vitro model of osteoblast development , 1992, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[12]  G. Stein,et al.  The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: modification of expression of genes supporting cell growth, adhesion, and extracellular matrix mineralization. , 1995, Experimental cell research.

[13]  T J Chambers,et al.  Stimulation of bone nodule formation in vitro by prostaglandins E1 and E2. , 1992, Endocrinology.

[14]  B D Boyan,et al.  Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells. , 1996, Journal of biomedical materials research.

[15]  John A. Robinson,et al.  Growth on type I collagen promotes expression of the osteoblastic phenotype in human osteosarcoma MG‐63 cells , 1992, Journal of cellular physiology.

[16]  I. Orly,et al.  The influence of calcium phosphate biomaterials on human bone cell activities. An in vitro approach. , 1990, Journal of biomedical materials research.

[17]  J. Kivilahti,et al.  Effect of surface processing on the attachment, orientation, and proliferation of human gingival fibroblasts on titanium. , 1992, Journal of biomedical materials research.

[18]  B. Shenker,et al.  Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2. , 1994, Developmental biology.

[19]  D. W. Johnson,et al.  Perovskite Oxides: Materials Science in Catalysis , 1977, Science.

[20]  O. H. Lowry,et al.  The quantitative histochemistry of brain. II. Enzyme measurements. , 1954, The Journal of biological chemistry.

[21]  R. Tuan,et al.  Enhanced extracellular matrix production and mineralization by osteoblasts cultured on titanium surfaces in vitro. , 1992, Journal of cell science.

[22]  B D Boyan,et al.  Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). , 1995, Journal of biomedical materials research.

[23]  J. Y. Martin,et al.  Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation. , 1996, Journal of biomedical materials research.

[24]  J. Davies,et al.  Guided bone tissue elaboration by osteogenic cells in vitro. , 1993, Journal of biomedical materials research.