Free-electron laser tissue-soldering in vitro with an albumin solder

The purpose of this study was to explore the feasibility of using a free-electron laser (FEL) to photothermally coagulate an albumin solder for laser-assisted incision closure. A 50%(w/v) bovine serum albumin solder was used to repair an incision in bovine aorta. The solder was coagulated by targeting absorption peaks in the solder infrared absorption spectrum using the FEL. Acute breaking strengths of repaired incisions were measured and the data analyzed by one-way ANOVA (P < 0.05). Multiple comparisons of means were performed using the Newman-Keuls test. The solder absorption spectrum from 2 - 10 microns was similar to water with an additional peak at 6.45 microns (amide II) due to the albumin. Preliminary results indicated that wavelengths at or very close to the absorption peaks were excessively absorbed, resulting in only the top surface of the solder being coagulated. Using wavelengths at points of weak absorption on the water absorption curve yielded better results.

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