Source of Neuraminidase in Human Whole Saliva

Whole saliva specimens from eight healthy human adults were examined for neuraminidase. The presence of two types of neuraminidase in four samples out of eight was demonstrated by means of polyacrylamide gel electrophoresis and sucrose density gradient centrifugation. One type is soluble and the other an insoluble, perhaps particle-bound, enzyme. The pH optima were 5.8 for the former and 5.0 to 5.3 for the latter. However, the soluble enzyme could not be detected in the other four saliva specimens which showed low activity. A comparative study of the salivary and other neuraminidases was carried out. It was found that both salivary neuraminidases were closely similar to the enzymes in submandibular-sublingual secretions and in human liver, but not to the oral streptococcal enzymes. The results suggest that the salivary neuraminidases might originate from cells such as epithelial cells or polymorphonuclear leukocytes, or both, in the oral cavity.

[1]  C. Schengrund,et al.  Localization of sialidase in the plasma membrane of rat liver cells. , 1972, The Journal of biological chemistry.

[2]  K. Fukui,et al.  Neuraminidase activity in some bacteria from the human mouth. , 1971, Archives of oral biology.

[3]  D. Tulsiani,et al.  Studies on the soluble and lysosomal neuraminidases of rat mammary glands. , 1971, Biochimica et biophysica acta.

[4]  A. Bahn,et al.  Carbohydrate hydrolases of oral streptococci. , 1969, Archives of oral biology.

[5]  E. Newbrun,et al.  Purification and properties of dextransucrase from Streptococcus sanguis. , 1969, Archives of oral biology.

[6]  A. Bahn,et al.  Extracellular Streptococcal Neuraminidase , 1968, Journal of bacteriology.

[7]  M. L. Hayes,et al.  Isolation in Pure Culture of Human Oral Organisms capable of producing Neuraminidase , 1967, Nature.

[8]  A. Tanaka,et al.  Streptococcal Sialidase I. Isolation and Properties of Sialidase Produced by Group K Streptococcus , 1967, Journal of bacteriology.

[9]  I. Glickman,et al.  Salivary neuraminidase. 3. Its relation to oral disease. , 1967, Journal of periodontology.

[10]  I. Glickman,et al.  Salivary neuraminidase. I. The presence of neuraminidase in human saliva. , 1966, Journal of periodontology.

[11]  J. C. Thonard,et al.  Neuraminidase Activity in Mixed Culture Supernatant Fluids of Human Oral Bacteria , 1965, Journal of bacteriology.

[12]  S. Leach,et al.  Release and Breakdown of Sialic Acid from Human Salivary Mucin and its Role in the Formation of Dental Plaque , 1963, Nature.

[13]  H. Lura An investigation into the relation between the sialic acid of saliva and dental caries. , 1961, Archives of oral biology.

[14]  W. A. Curby Device for collection of human parotid saliva. , 1953, The Journal of laboratory and clinical medicine.

[15]  C. Dawes,et al.  Neuraminidase activity in the salivary glands of rats and human saliva. , 1970, Archives of oral biology.

[16]  J. Carlsson Presence of various types of non-haemolytic streptococci in dental plaque and in other sites of the oral cavity in man. , 1967, Odontologisk revy.

[17]  G. Roella,et al.  Neuraminidase in Human Sputum , 1966 .

[18]  C. Dawes,et al.  The effects of different stimuli on the composition of saliva in man , 1964, The Journal of physiology.