Time-domain diffuse fluorescence tomography of CT-analogous scheme: an experimental validation

A fiber-based non-contact scheme of the time-domain diffuse fluorescence yield and lifetime tomography is described that combines the time-correlated single photon counting technique for high-sensitive, time-resolved detection and CT-analogous configuration for high throughput data collection. A pilot validation of the methodology is performed for two-dimensional scenarios using simulated and experimental data. The results demonstrated the potential of the proposed scheme in improving the image quality.

[1]  Arridge,et al.  Boundary conditions for light propagation in diffusive media with nonscattering regions , 2000, Journal of the Optical Society of America. A, Optics, image science, and vision.

[2]  Feng Gao,et al.  Simultaneous fluorescence yield and lifetime tomography from time-resolved transmittances of small-animal-sized phantom. , 2010, Applied optics.

[3]  Zhao Hui-juan.  A Linear Featured-data Scheme for Image Reconstruction in Time-domain Fluorescence Molecular Tomography , 2008 .

[4]  Scott C. Davis,et al.  Preclinical Whole-body Fluorescence Imaging: Review of Instruments, Methods and Applications , 2013 .

[5]  Xiaoquan Yang,et al.  Fluorescence molecular tomography system for in vivo tumor imaging in small animals , 2010 .

[6]  Jie Tian,et al.  Efficient reconstruction method for L1 regularization in fluorescence molecular tomography. , 2010, Applied optics.

[7]  Vasilis Ntziachristos,et al.  Looking and listening to light: the evolution of whole-body photonic imaging , 2005, Nature Biotechnology.

[8]  Hamid Dehghani,et al.  A microcomputed tomography guided fluorescence tomography system for small animal molecular imaging. , 2009, The Review of scientific instruments.

[9]  Vasilis Ntziachristos,et al.  A submillimeter resolution fluorescence molecular imaging system for small animal imaging. , 2003, Medical physics.

[10]  R. Weissleder,et al.  Fluorescence molecular tomography resolves protease activity in vivo , 2002, Nature Medicine.

[11]  Scott C Davis,et al.  Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applications. , 2010, Journal of photochemistry and photobiology. B, Biology.

[12]  Mark A A Neil,et al.  Fluorescence lifetime imaging by using time-gated data acquisition. , 2007, Applied optics.

[13]  Feng Gao,et al.  A fiber-based, non-contact scheme for time-domain diffuse fluorescence tomography: methodology and simulative validation , 2010, BiOS.

[14]  J. Bai,et al.  In vivo whole-body imaging of optical agent dynamics using full angle fluorescence diffuse optical tomography , 2010 .