3D Visualization of iron oxide nanoparticles in MRI of inflammatory model

Nanomedicine is becoming an extremely promising research area for healthcare. The visualization and quantification of nanoparticles (NPs) inside the organs of interest pose a significant challenge and therefore, novel image processing approaches are required for a better diagnosis. The purpose of this work was to develop a novel approach for better visualization and quantification of iron oxide NPs in three dimension (3D) high-resolution magnetic resonance (MR) images of an inflammatory model. The proposed procedure focuses on the extraction of NPs from the background surrounding it. It is applied on 2D and 3D images and is based on pre-processing and segmentation by automatic threshold to visualize the NPs inside the mouse calf using a control set of images of the same calf before injecting the NPs. The resulting visualization of the 3D distribution of iron oxide NPs inside the inflamed area of the calf has a potential in the advancement of NPs application in nanomedicine therapy and diagnosis.Graphical abstract

[1]  Bülent Sankur,et al.  Survey over image thresholding techniques and quantitative performance evaluation , 2004, J. Electronic Imaging.

[2]  L Trahms,et al.  Quantification of magnetic nanoparticles by magnetorelaxometry and comparison to histology after magnetic drug targeting. , 2006, Journal of nanoscience and nanotechnology.

[3]  Brian K Rutt,et al.  Imaging single mammalian cells with a 1.5 T clinical MRI scanner , 2003, Magnetic resonance in medicine.

[4]  Jens Rittscher,et al.  Spatio-temporal cell cycle phase analysis using level sets and fast marching methods , 2009, Medical Image Anal..

[5]  F. Gazeau,et al.  In vivo cellular imaging of lymphocyte trafficking by MRI: A tumor model approach to cell‐based anticancer therapy , 2006, Magnetic resonance in medicine.

[6]  Takeo Kanade,et al.  Cell population tracking and lineage construction with spatiotemporal context , 2008, Medical Image Anal..

[7]  Miqin Zhang,et al.  Superparamagnetic iron oxide nanoparticle-based delivery systems for biotherapeutics , 2013, Expert opinion on drug delivery.

[8]  J. Frank,et al.  Cellular MRI and its role in stem cell therapy. , 2008, Regenerative medicine.

[9]  J. Frank,et al.  In Vivo Cellular Imaging for Translational Medical Research. , 2009, Current medical imaging reviews.

[10]  Huw D. Summers,et al.  Automated Cell Identification and Tracking Using Nanoparticle Moving-Light-Displays , 2012, PloS one.

[11]  Ajay Kumar Gupta,et al.  Recent advances on surface engineering of magnetic iron oxide nanoparticles and their biomedical applications. , 2007, Nanomedicine.

[12]  Jelena Kolosnjaj-Tabi,et al.  Real-time high-resolution magnetic resonance tracking of macrophage subpopulations in a murine inflammation model: a pilot study with a commercially available cryogenic probe. , 2013, Contrast media & molecular imaging.

[13]  R. Weissleder,et al.  In vivo high resolution three-dimensional imaging of antigen-specific cytotoxic T-lymphocyte trafficking to tumors. , 2003, Cancer research.

[14]  Harish Poptani,et al.  In vivo and ex vivo MRI detection of localized and disseminated neural stem cell grafts in the mouse brain , 2005, NeuroImage.

[15]  Wen-Nung Lie,et al.  Automatic target segmentation by locally adaptive image thresholding , 1995, IEEE Trans. Image Process..

[16]  Jeff W M Bulte,et al.  Iron oxide MR contrast agents for molecular and cellular imaging , 2004, NMR in biomedicine.

[17]  T. Schaeffter,et al.  Limits of detection of SPIO at 3.0 T using T2* relaxometry , 2005, Magnetic resonance in medicine.

[18]  Morteza Mahmoudi,et al.  Magnetic fluid hyperthermia: focus on superparamagnetic iron oxide nanoparticles. , 2011, Advances in colloid and interface science.

[19]  S. Shalev,et al.  BIOMEDICAL IMAGE PROCESSING WITH THE DICOM-8. , 1985 .

[20]  Piotr Walczak,et al.  Tracking stem cells using magnetic nanoparticles. , 2011, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[21]  Mathias Hoehn,et al.  Cellular MR Imaging , 2005, Molecular imaging.

[22]  Wei Liu,et al.  Detection and quantification of magnetically labeled cells by cellular MRI. , 2009, European journal of radiology.

[23]  Sumit Arora,et al.  Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers , 2012, International journal of nanomedicine.

[24]  P. Walczak,et al.  Applicability and limitations of MR tracking of neural stem cells with asymmetric cell division and rapid turnover: The case of the Shiverer dysmyelinated mouse brain , 2007, Magnetic resonance in medicine.

[25]  Qun Zhong,et al.  Security Control for COTS Components , 1998, Computer.

[26]  M. M. Ahmed,et al.  A Study on the Validation of Histogram Equalization as a Contrast Enhancement Technique , 2012, 2012 International Conference on Advanced Computer Science Applications and Technologies (ACSAT).