The use of RAPD-PCR analysis in characterization of Liriomyza trifolii (Burgess, 1880), Liriomyza congesta (Becker 1903), Agromyza apfelbecki Strobl, 1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey

The Liriomyza trifolii (Burgess, 1880), Liriomya congesta (Becker, 1903), Agromyza apfelbecki Strobl, 1902 and Chromatomyia horticola (Goureau, 1851) species have become important vegetable pests in the agricultural areas in Turkey. These species are not easily differentiated by their morphological characteristics. The aim of the present study was to obtain genetic markers to unambiguously distinguish these species and gain insight into genetic variation between the individuals of L. trifolii strains collected from various geographic locations in Turkey. Thus, four random primers were employed to generate RAPD markers. Different RAPD profiles were observed for the different species indicating that the RAPD-PCR analysis can be applied as a useful tool in quickly screening the strains to aid in discriminating these species that was routinely done via classical methods. Identification of biotypes of the species is crucial in order to designing control strategies to avoid the spread of the pests because of economic losses caused by the damages to vegetable crops. Our results show that RAPD is promisingly an effective, fast and economic way, hence proposed as a valuable alternative to traditional identification of the insect species and strains.

[1]  M. Černý.  Additional new records of Agromyzidae (Diptera) from the Palaearctic Region , 2006, Acta Musei Silesiae, Scientiae Naturales.

[2]  Emine Çıkman,et al.  Contributions to the leafminer fauna (Diptera: Agromyzidae) from Turkey, with four new records , 2005, Phytoparasitica.

[3]  L. Kox,et al.  Identification of economically important Liriomyza species by PCR-RFLP analysis * , 2005 .

[4]  C. Mart,et al.  Contributions to the Agromyzidae (Diptera) Fauna of Turkey , 2005 .

[5]  H. Sungur.  Two new records for the Turkish Agromyzidae (Diptera) fauna , 2004 .

[6]  R. Hallett,et al.  Polymerase Chain Reaction-Restriction Fragment-Length Polymorphism Method to Distinguish Liriomyza huidobrensis from L. langei (Diptera: Agromyzidae) Applied to Three Recent Leafminer Invasions , 2001, Journal of economic entomology.

[7]  S. Scheffer,et al.  Two Nuclear Genes Confirm Mitochondrial Evidence of Cryptic Species within Liriomyza huidobrensis (Diptera: Agromyzidae) , 2001 .

[8]  S. Scheffer.  Molecular Evidence of Cryptic Species within the Liriomyza huidobrensis (Diptera: Agromyzidae) , 2000, Journal of economic entomology.

[9]  J. Deeming,et al.  Some new records for Turkish leafminers (Diptera: Agromyzidae) fauna from Izmir province , 2000 .

[10]  N. Uygun,et al.  Faunistic studies on Agromyzidae (Diptera) in the south east mediterranean region of Turkey , 1995 .

[11]  A. Broadbent,et al.  Wounds caused by Liriomyza trifolii (Diptera: Agromyzidae) as sites for infection of chrysanthemum by Pseudomonas cichorii. , 1988 .

[12]  J. V. Lenteren,et al.  The leafminers Liriomyza bryoniae and L. trifolii (Diptera: Agromyzidae), their parasites and host plants; a review , 1986 .

[13]  J. Trumble,et al.  Reduction of Tomato Leaflet Photosynthesis Rates by Mining Activity of Liriomyza sativae (Diptera: Agromyzidae) , 1983 .

[14]  D. Hogness,et al.  Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. , 1983, Journal of molecular biology.

[15]  K. Spencer.  The Agromyzidae (Diptera) of Fennoscandia and Denmark , 1976 .

[16]  C. H. Lindroth.  Handbooks for the identification of British insects. Coleoptera, Carabidae. , 1974 .

[17]  K. Spencer.  Agromyzidae (Diptera) of Economic Importance , 1973, Series Entomologica.