Development of novel alginate based hydrogel films for wound healing applications.

Alginate and Aloe vera are natural materials widely investigated and used in the biomedical field. In this research work, thin hydrogel films composed by alginate and Aloe vera gel in different proportions (95:5, 85:15 and 75:25, v/v) were prepared and characterized. The films were evaluated regarding the light transmission behavior, contact angle measurements, and chemical, thermal and mechanical properties. These thin hydrogel films, prepared by crosslinking reaction using 5% calcium chloride solution, were also investigated relatively to their water solubility and swelling behavior. Results showed that Aloe vera improved the transparency of the films, as well their thermal stability. The developed films present adequate mechanical properties for skin applications, while the solubility studies demonstrated the insolubility of the films after 24h of immersion in distilled water. The water absorption and swelling behavior of these films were greatly improved by the increase in Aloe vera proportion.

[1]  S. Simal,et al.  Effects of heat treatment and dehydration on bioactive polysaccharide acemannan and cell wall polymers from Aloe barbadensis Miller , 2003 .

[2]  P. Supaphol,et al.  Preparation and characterization of asiaticoside-loaded alginate films and their potential for use as effectual wound dressings , 2011 .

[3]  W. Yi.,et al.  Isolation, chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water , 2007 .

[4]  Y. Suzuki,et al.  In vivo evaluation of a novel alginate dressing. , 1999, Journal of biomedical materials research.

[5]  Rubens Maciel Filho,et al.  Rheological behavior of alginate solutions for biomanufacturing , 2009 .

[6]  Eugene Khor,et al.  Chitosan-alginate PEC membrane as a wound dressing: Assessment of incisional wound healing. , 2002, Journal of biomedical materials research.

[7]  D. Mooney,et al.  Alginate: properties and biomedical applications. , 2012, Progress in polymer science.

[8]  B. Rehm.  Alginates: Biology and Applications , 2009 .

[9]  B. Fawcett.  The relation of the factor of growth to the pathogenesis of megacolon, megaileum, and megaduodenum. , 1951, Surgery.

[10]  Lai Wah Chan,et al.  Mechanisms of external and internal gelation and their impact on the functions of alginate as a coat and delivery system , 2006 .

[11]  É. Cavalheiro,et al.  Thermal behavior of alginic acid and its sodium salt , 2004, Ecletica Quimica.

[12]  A. Bée,et al.  Preparation and effect of Ca2+ on water solubility, particle release and swelling properties of magnetic alginate films , 2006 .

[13]  S. Engelsen,et al.  Alginate monomer composition studied by solution- and solid-state NMR – A comparative chemometric study , 2009 .

[14]  J. Chen,et al.  A preliminary in vitro study on the fabrication and tissue engineering applications of a novel chitosan bilayer material as a scaffold of human neofetal dermal fibroblasts. , 2001, Biomaterials.

[15]  Y. Shimada,et al.  Aloe vera oral administration accelerates acute radiation-delayed wound healing by stimulating transforming growth factor-β and fibroblast growth factor production. , 2011, American journal of surgery.

[16]  S. Puttipipatkhachorn,et al.  Investigation of novel alginate-magnesium aluminum silicate microcomposite films for modified-release tablets. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[17]  J. Coey,et al.  Magnetism and Magnetic Materials , 2001 .

[18]  Joseph Jagur-Grodzinski,et al.  Polymeric gels and hydrogels for biomedical and pharmaceutical applications , 2010 .

[19]  K. Chennazhi,et al.  Sodium alginate/poly(vinyl alcohol)/nano ZnO composite nanofibers for antibacterial wound dressings. , 2011, International journal of biological macromolecules.

[20]  David J Mooney,et al.  Alginate hydrogels as biomaterials. , 2006, Macromolecular bioscience.

[21]  H. Thu,et al.  Alginate based bilayer hydrocolloid films as potential slow-release modern wound dressing. , 2012, International journal of pharmaceutics.

[22]  F. Beland,et al.  An Evaluation of the Biological and Toxicological Properties of Aloe Barbadensis (Miller), Aloe Vera , 2006, Journal of environmental science and health. Part C, Environmental carcinogenesis & ecotoxicology reviews.

[23]  C. Chiu,et al.  Development of two alginate-based wound dressings , 2008, Journal of materials science. Materials in medicine.

[24]  T. Reynolds.  Aloes: The genus Aloe , 2004 .

[25]  P. Sriamornsak,et al.  Swelling and diffusion studies of calcium polysaccharide gels intended for film coating. , 2008, International journal of pharmaceutics.

[26]  G. Jemec,et al.  The mechanical properties of skin in osteogenesis imperfecta. , 2002, Archives of dermatology.

[27]  P. Quéré,et al.  In vivo macrophage activation in chickens with Acemannan, a complex carbohydrate extracted from Aloe vera. , 2000, International journal of immunopharmacology.

[28]  Changren Zhou,et al.  Polysaccharides-based nanoparticles as drug delivery systems. , 2008, Advanced drug delivery reviews.

[29]  S. Puttipipatkhachorn,et al.  Alginate-magnesium aluminum silicate films: effect of plasticizers on film properties, drug permeation and drug release from coated tablets. , 2007, International journal of pharmaceutics.

[30]  J. Boateng,et al.  Wound healing dressings and drug delivery systems: a review. , 2008, Journal of pharmaceutical sciences.

[31]  Todd L. Cecil,et al.  FIP/AAPS Joint Workshop Report: Dissolution/In Vitro Release Testing of Novel/Special Dosage Forms , 2011, AAPS PharmSciTech.

[32]  M. Zilberman,et al.  Novel biodegradable composite wound dressings with controlled release of antibiotics: results in a guinea pig burn model. , 2011, Burns : journal of the International Society for Burn Injuries.

[33]  Paola Laurienzo,et al.  Marine Polysaccharides in Pharmaceutical Applications: An Overview , 2010, Marine drugs.

[34]  J. P. Feitosa,et al.  Extraction and physicochemical characterization of Sargassum vulgare alginate from Brazil. , 2007, Carbohydrate research.

[35]  K. Draget,et al.  Alginate based new materials. , 1997, International journal of biological macromolecules.

[36]  J. Kennedy,et al.  Alginate/starch blend fibers and their properties for drug controlled release , 2010 .

[37]  Xiaobing Fu,et al.  Naturally derived materials-based cell and drug delivery systems in skin regeneration. , 2010, Journal of controlled release : official journal of the Controlled Release Society.

[38]  W. Banlunara,et al.  Acemannan stimulates gingival fibroblast proliferation; expressions of keratinocyte growth factor-1, vascular endothelial growth factor, and type I collagen; and wound healing. , 2009, Journal of pharmacological sciences.

[39]  P. Ferreira,et al.  Photocrosslinkable biodegradable responsive hydrogels as drug delivery systems. , 2011, International journal of biological macromolecules.

[40]  Paulo Jorge Da Silva bartolo,et al.  Preparation and Characterization of Films Based on Alginate and Aloe Vera , 2011 .

[41]  J. Benoit,et al.  5-ASA loaded chitosan-Ca-alginate microparticles: Preparation and physicochemical characterization. , 2007, International journal of pharmaceutics.

[42]  R. Pandey,et al.  Antibacterial Activities of Crude Extract of Aloe barbadensis to Clinically Isolated Bacterial Pathogens , 2010, Applied biochemistry and biotechnology.

[43]  Ki Myong Kim,et al.  Comparative studies on the characterization and antioxidant properties of biodegradable alginate films containing ginseng extract , 2010 .

[44]  L. DiPietro,et al.  Factors Affecting Wound Healing , 2010, Journal of dental research.

[45]  Federica Chiellini,et al.  Polymeric Materials for Bone and Cartilage Repair , 2010 .