Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers

Abstract Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.

[1]  H. Jung,et al.  Characterization of Species of Cladobotryum which Cause Cobweb Disease in Edible Mushrooms Grown in Korea , 2012, Mycobiology.

[2]  H. Ro,et al.  Determination of Mineral Components in the Cultivation Substrates of Edible Mushrooms and Their Uptake into Fruiting Bodies , 2009, Mycobiology.

[3]  F. J. Gea,et al.  Brown Blotch Caused by Pseudomonas tolaasii on Cultivated Pleurotus eryngii in Spain. , 2009, Plant disease.

[4]  Won Noh,et al.  Isolation of Bacteria Associated with the King Oyster Mushroom, Pleurotus eryngii , 2008, Mycobiology.

[5]  Irina S Druzhinina,et al.  Genetically Closely Related but Phenotypically Divergent Trichoderma Species Cause Green Mold Disease in Oyster Mushroom Farms Worldwide , 2007, Applied and Environmental Microbiology.

[6]  T. Lee,et al.  Isolation and characterization of a novel mycovirus, PeSV, in Pleurotus eryngii and the development of a diagnostic system for it , 2006, Biotechnology Letters.

[7]  Paul T Rygiewicz,et al.  Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts , 2005, BMC Microbiology.

[8]  D. Lim,et al.  Characterization of a novel single-stranded RNA mycovirus in Pleurotus ostreatus. , 2003, Virology.

[9]  I. Choi,et al.  Physiological Characteristics of Green Mold (Trichoderma spp.) Isolated from Oyster Mushroom (Pleurotus spp.) , 2003 .

[10]  B. Adie,et al.  Double-stranded RNA elements associated with the MVX disease of Agaricus bisporus. , 2003, Mycological research.

[11]  P. Crous,et al.  Species of Phomopsis and a Libertella sp. occurring on grapevines with specific reference to South Africa: morphological, cultural, molecular and pathological characterization , 2001 .

[12]  C. Ciccarone,et al.  Pests and polluting organisms in Pleurotus eryngii crop , 1998 .

[13]  M. Goodin,et al.  Encapsidation of the La France disease-specific double-stranded RNAs in 36-nm isometric viruslike particles , 1992 .

[14]  Jr. W. L. Smith.  Studies withMucorSpecies Causing Postharvest Decay of Fresh Produce , 1979 .