Studies on antibody avidity at the cellular level. Effects of immunological paralysis and administered antibody

The avidity of antibodies produced by single cells has been studied using inhibition of hemolytic plaque formation by free antigen as a test system. The effect of a paralyzing injection of antigen into mice immunized with heterologous albumins was studied. After a lag period of approximately two days the number of plaque‐forming cells (PFC) decreased in the paralyzed mice. The PFC that remained in the paralyzed animals released antibodies with a low avidity for the antigen as compared to control PFC. The impact of an injection of antibody into immune mice was then studied. A reduction in the number of PFC was found in the antiserum suppressed animals. The remaining PFC released antibody of a relatively high avidity for the antigen. The data support the concept of a similar binding constant for the cell‐associated antibody‐like receptor operating in induction of immunity and paralysis and the antibody released from the cell after stimulation.

[1]  J. Uhr,et al.  REGULATION OF ANTIBODY FORMATION BY SERUM ANTIBODY : II. REMOVAL OF SPECIFIC ANTIBODY BY MEANS OF EXCHANGE TRANSFUSION , 1970 .

[2]  M. Rittenberg,et al.  In vitro-initiated secondary anti-hapten response. II. Increasing cell avidity for antigen. , 1970 .

[3]  B. Andersson.  STUDIES ON THE REGULATION OF AVIDITY AT THE LEVEL OF THE SINGLE ANTIBODY-FORMING CELL , 1970, The Journal of experimental medicine.

[4]  W. Paul,et al.  Hapten specificity of cellular immune responses as compared with the specificity of serum anti-hapten antibody. , 1970, Immunology.

[5]  H. A. Levin,et al.  Studies on the specificity and affinity of alpha, DNP-oligolysine antibody: a basis for questioning the role of cell-bound antibody in cellular recognition of antigen. , 1970, Journal of immunology.

[6]  G. Habicht,et al.  Cellular sites of immunologic unresponsiveness. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A. Yamada,et al.  2,4-dinitrophenyl-hapten specific hemolytic plaque-in-gel formation by mouse myeloma (MOPC-315) cells. , 1970, Journal of immunology.

[8]  J. Uhr,et al.  The effect of dose of antigen on protein and DNA synthesis in antibody-forming cells. , 1969, Journal of immunology.

[9]  F. Celada,et al.  Determination of avidity of anti-albumin antibodies in the mouse. Influence of the number of cells transferred on the quality of the secondary adoptive response. , 1969, Immunology.

[10]  S. Britton.  Regulation of antibody synthesis against Escherichia coli endotoxin. 3. Induction of immunological paralysis in non-immune and pre-immunized mice. , 1969, Immunology.

[11]  J. Miller,et al.  CELL TO CELL INTERACTION IN THE IMMUNE RESPONSE , 1968, The Journal of experimental medicine.

[12]  G. Siskind,et al.  Selection of cell populations in induction of tolerance: affinity of antibody formed in partially tolerant rabbits. , 1968, Journal of immunology.

[13]  H. Wigzell.  ANTIBODY SYNTHESIS AT THE CELLULAR LEVEL ANTIBODY-INDUCED SUPPRESSION OF 7S ANTIBODY SYNTHESIS , 1966 .

[14]  B. Andersson,et al.  Studies at the cellular level of the 19S immune response. , 1966, Acta pathologica et microbiologica Scandinavica.

[15]  M. Brooke.  Breaking of Immunological Paralysis by Injection of a Specific Depolymerase , 1964, Nature.

[16]  J. Uhr,et al.  THE HETEROGENEITY OF THE IMMUNE RESPONSE. , 1964, Science.

[17]  H. Eisen,et al.  VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. , 1964, Biochemistry.

[18]  F. Greenwood,et al.  Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity , 1962, Nature.

[19]  G. Thorbecke,et al.  The induction in mice of sensitization and immunological unresponsiveness by neonatal injection of bovine gamma-globulin. , 1961, Journal of immunology.

[20]  R. Farr,et al.  A quantitative immunochemical measure of the primary interaction between I BSA and antibody. , 1958, The Journal of infectious diseases.

[21]  Floyd Tm,et al.  Studies on experimental shigellosis. II. The effect of fasting and fatigue on Shigella flexneri 3 infections in mice. , 1958 .

[22]  A. Pickard,et al.  Partial tolerance to the dinitrophenyl group in neonatal and adult mice. , 1970, International archives of allergy and applied immunology.

[23]  W. Paul,et al.  SPECIFICITY OF CELLULAR IMMUNE RESPONSES , 1968, The Journal of experimental medicine.

[24]  J. G. Walker,et al.  STUDIES ON THE CONTROL OF ANTIBODY SYNTHESIS II. EFFECT OF ANTIGEN DOSE AND OF SUPPRESSION BY PASSIVE ANTIBODY ON THE AFFINITY OF ANTIBODY SYNTHESIZED , 1968 .

[25]  G. Biozzi,et al.  A kinetic study of antibody producing cells in the spleen of mice immunized intravenously with sheep erythrocytes. , 1968, Immunology.

[26]  H. Eisen,et al.  Symposium on in vitro studies of the immune response. I. Variations in the immune response to a simple determinant. , 1966, Bacteriological reviews.

[27]  S. Svehag,et al.  THE FORMATION AND PROPERTIES OF POLIOVIRUS-NEUTRALIZING ANTIBODY. II. 19S AND 7S ANTIBODY FORMATION: DIFFERENCES IN ANTIGEN DOSE REQUIREMENT FOR SUSTAINED SYNTHESIS, ANAMNESIS, AND SENSITIVITY TO X-IRRADIATION. , 1964 .