A new isolation method for bacterial extracellular vesicles providing greater purity and improved proteomic detection of vesicle proteins

[1]  L. Michel,et al.  Outer membrane vesicles as molecular biomarkers for Gram-negative sepsis: Taking advantage of nature’s perfect packages , 2022, The Journal of biological chemistry.

[2]  M. Brucale,et al.  Comparison of separation methods for immunomodulatory extracellular vesicles from helminths , 2022, Journal of Extracellular Biology.

[3]  A. Handberg,et al.  Lipid‐based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis , 2022, Journal of extracellular vesicles.

[4]  M. Ullah,et al.  Methodologies to Isolate and Purify Clinical Grade Extracellular Vesicles for Medical Applications , 2022, Cells.

[5]  R. Zain,et al.  Growth Media Conditions Influence the Secretion Route and Release Levels of Engineered Extracellular Vesicles , 2021, Advanced healthcare materials.

[6]  M. Kandušer,et al.  Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization , 2021, Frontiers in Pharmacology.

[7]  L. Margolis,et al.  Microbiota–host communications: Bacterial extracellular vesicles as a common language , 2021, PLoS pathogens.

[8]  Deng-Chyang Wu,et al.  Outer Membrane Vesicle Production by Helicobacter pylori Represents an Approach for the Delivery of Virulence Factors CagA, VacA and UreA into Human Gastric Adenocarcinoma (AGS) Cells , 2021, International journal of molecular sciences.

[9]  Angela C. Brown,et al.  Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity. , 2021, Journal of visualized experiments : JoVE.

[10]  A. Baccarelli,et al.  Isolation and characterization of extracellular vesicles in saliva of children with asthma , 2021, Extracellular vesicles and circulating nucleic acids.

[11]  Patrick M. Chong,et al.  Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content , 2021, Frontiers in Microbiology.

[12]  M. Fiandaca,et al.  Foetal bovine serum influence on in vitro extracellular vesicle analyses , 2021, Journal of extracellular vesicles.

[13]  J. Park,et al.  Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation , 2020, The Korean journal of internal medicine.

[14]  S. van der Veen,et al.  Role of Outer Membrane Vesicles in Bacterial Physiology and Host Cell Interactions , 2020 .

[15]  Anup D Shah,et al.  LFQ-Analyst: An easy-to-use interactive web-platform to analyze and visualize label-free proteomics data preprocessed with MaxQuant. , 2020, Journal of proteome research.

[16]  M. Stevens,et al.  Experimental artefacts can lead to misattribution of bioactivity from soluble mesenchymal stem cell paracrine factors to extracellular vesicles , 2020, Journal of extracellular vesicles.

[17]  O. Kretz,et al.  Management of Tamm–Horsfall Protein for Reliable Urinary Analytics , 2019, Proteomics. Clinical applications.

[18]  C. Blenkiron,et al.  Analysis of the Escherichia coli extracellular vesicle proteome identifies markers of purity and culture conditions , 2019, Journal of extracellular vesicles.

[19]  Zhenjia Wang,et al.  Outer membrane vesicles for vaccination and targeted drug delivery. , 2019, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[20]  D. Greening,et al.  Helicobacter pylori Growth Stage Determines the Size, Protein Composition, and Preferential Cargo Packaging of Outer Membrane Vesicles , 2018, Proteomics.

[21]  Jing Xu,et al.  Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines , 2018, Journal of Extracellular Vesicles.

[22]  P. Forterre,et al.  Extracellular membrane vesicles in the three domains of life and beyond , 2018, FEMS microbiology reviews.

[23]  David M Lubman,et al.  Comparison of an Optimized Ultracentrifugation Method versus Size-Exclusion Chromatography for Isolation of Exosomes from Human Serum. , 2018, Journal of proteome research.

[24]  A. Hill,et al.  Helicobacter pylori Outer Membrane Vesicle Size Determines Their Mechanisms of Host Cell Entry and Protein Content , 2018, Front. Immunol..

[25]  J. Silke,et al.  NOD1 is required for Helicobacter pylori induction of IL‐33 responses in gastric epithelial cells , 2018, Cellular microbiology.

[26]  P. Laktionov,et al.  Isolation of Extracellular Vesicles: General Methodologies and Latest Trends , 2018, BioMed research international.

[27]  C. Blenkiron,et al.  Isolation of membrane vesicles from prokaryotes: a technical and biological comparison reveals heterogeneity , 2017, Journal of extracellular vesicles.

[28]  Daisuke Ejima,et al.  Protein aggregation under high concentration/density state during chromatographic and ultrafiltration processes. , 2017, International journal of biological macromolecules.

[29]  Jüergen Cox,et al.  The MaxQuant computational platform for mass spectrometry-based shotgun proteomics , 2016, Nature Protocols.

[30]  A. Geldof,et al.  Protein Complexes in Urine Interfere with Extracellular Vesicle Biomarker Studies , 2016, Journal of circulating biomarkers.

[31]  Igor L. Medintz,et al.  Emerging therapeutic delivery capabilities and challenges utilizing enzyme/protein packaged bacterial vesicles. , 2015, Therapeutic delivery.

[32]  M. Kaparakis-Liaskos,et al.  Immune modulation by bacterial outer membrane vesicles , 2015, Nature Reviews Immunology.

[33]  A. Brisson,et al.  High-speed centrifugation induces aggregation of extracellular vesicles , 2015, Journal of extracellular vesicles.

[34]  Chris Gardiner,et al.  Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis , 2013, Journal of extracellular vesicles.

[35]  Aled Clayton,et al.  How pure are your vesicles? , 2013, Journal of extracellular vesicles.

[36]  Lynne T. Bemis,et al.  Standardization of sample collection, isolation and analysis methods in extracellular vesicle research , 2013, Journal of extracellular vesicles.

[37]  M. Mann,et al.  Andromeda: a peptide search engine integrated into the MaxQuant environment. , 2011, Journal of proteome research.

[38]  J. Keenan,et al.  Outer membrane vesicles enhance the carcinogenic potential of Helicobacter pylori. , 2008, Carcinogenesis.

[39]  J. Klimentova,et al.  Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria. , 2015, Microbiological research.