In the work the fluorescence spectroscopy based on fluorescence spectra for single excitation wavelength and excitation spectra for single emission wavelength were applied to expand the knowledge about the specific signatures of lubricate oil typically used in ship engine as a tool to oil quality assessment. The fluorescence properties of oil are considered based on an exemplary lubricate-engine oil in two forms: fresh and used oil – exploited an adequate time. Based on the fluorescence spectra for single excitation wavelength and excitation spectra for single emission wavelength the changes of the spectra for both forms of oil are discussed and the peaks typical for both forms of oil were determined. Moreover the specified fluorescence signatures described by selected fluorescence spectrum for excitation wavelength 220 nm as a tool to distinguish the two forms of oil were determined. Additionally, there is discussed the use of total excitation spectrum as a tool to identification two forms of oil.
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