Sensitive point-of-care monitoring of cardiac biomarker myoglobin using aptamer and ubiquitous personal glucose meter.

Myoglobin (Myo), which is one of the early markers to increase after acute myocardial infarction (AMI), plays a major role in urgent diagnosis of cardiovascular diseases. Hence, monitoring of Myo in point-of-care is fundamental. Here, a novel assay for sensitive and selective detection of Myo was introduced using a personal glucose meter (PGM) as readout. In the presence of Myo, the anti-Myo antibody immobilized on the surface of polystyrene microplate could capture the target Myo. Then the selected aptamer against Myo, which was obtained using our screening process, was conjugated with invertase, and such aptamer-invertase conjugates bound to the immobilized Myo due to the Myo/aptamer interaction. Subsequently, the resulting "antibody-Myo-aptamer sandwich" complex containing invertase conjugates hydrolyzed sucrose into glucose, thus establishing direct correlation between the Myo concentration and the amount of glucose measured by PGM. By employing the enzyme amplification, as low as 50 pM Myo could be detected. This assay also showed high selectivity for Myo and was successfully used for Myo detection in serum samples. This work may provide a simple but reliable tool for early diagnosis of AMI in the world, especially in developing countries.

[1]  Kemin Wang,et al.  Multiplex detection of nucleic acids using a low cost microfluidic chip and a personal glucose meter at the point-of-care. , 2014, Chemical communications.

[2]  R. D. de Winter,et al.  Value of myoglobin, troponin T, and CK-MBmass in ruling out an acute myocardial infarction in the emergency room. , 1995, Circulation.

[3]  Stephen M Downs,et al.  The HealthPia GlucoPack Diabetes phone: a usability study. , 2007, Diabetes technology & therapeutics.

[4]  Devendra Kumar,et al.  Electrochemical impedance spectroscopy characterization of mercaptopropionic acid capped ZnS nanocrystal based bioelectrode for the detection of the cardiac biomarker--myoglobin. , 2012, Bioelectrochemistry.

[5]  Zhenyu Lin,et al.  Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout. , 2014, Biosensors & bioelectronics.

[6]  Tina M. Battaglia,et al.  Quantitative measurement of cardiac markers in undiluted serum. , 2007, Analytical chemistry.

[7]  Feng Zhang,et al.  Evaluation of a modified lateral flow immunoassay for detection of high-sensitivity cardiac troponin I and myoglobin. , 2013, Biosensors & bioelectronics.

[8]  A. Parente,et al.  Myoglobin as marker in meat adulteration: a UPLC method for determining the presence of pork meat in raw beef burger. , 2013, Food chemistry.

[9]  Scott T Phillips,et al.  Reagents and assay strategies for quantifying active enzyme analytes using a personal glucose meter. , 2013, Chemical communications.

[10]  Yun Xiang,et al.  Sensitive detection of copper(II) by a commercial glucometer using click chemistry. , 2013, Biosensors & bioelectronics.

[11]  Ki-Hwan Park,et al.  A facile and sensitive method for detecting pathogenic bacteria using personal glucose meters , 2013 .

[12]  Kemin Wang,et al.  Screening of DNA aptamers against myoglobin using a positive and negative selection units integrated microfluidic chip and its biosensing application. , 2014, Analytical chemistry.

[13]  Xianfeng Zhang,et al.  Iminodiacetic acid-functionalized gold nanoparticles for optical sensing of myoglobin via Cu2+ coordination. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[14]  Z. Song,et al.  Assay of femtogram level nitrite in human urine using luminol–myoglobin chemiluminescence , 2006 .

[15]  Yi Lu,et al.  An invasive DNA approach toward a general method for portable quantification of metal ions using a personal glucose meter. , 2013, Chemical communications.

[16]  Kemin Wang,et al.  Enhanced surface plasmon resonance with the modified catalytic growth of Au nanoparticles. , 2007, Biosensors & bioelectronics.

[17]  Kemin Wang,et al.  Recent advances in fluorescent nucleic acid probes for living cell studies. , 2013, The Analyst.

[18]  Yifan Ma,et al.  Dextran based sensitive theranostic nanoparticles for near-infrared imaging and photothermal therapy in vitro. , 2013, Chemical communications.

[19]  J. Lakowicz,et al.  Myoglobin immunoassay utilizing directional surface plasmon-coupled emission. , 2004, Analytical chemistry.

[20]  S. Yin,et al.  Detection of 4-hydroxy-2-nonenal adducts of turkey and chicken myoglobins using mass spectrometry. , 2010 .

[21]  A. Cass,et al.  Smart plastic antibody material (SPAM) tailored on disposable screen printed electrodes for protein recognition: application to myoglobin detection. , 2013, Biosensors & bioelectronics.

[22]  Yi Lu,et al.  Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets. , 2011, Nature chemistry.

[23]  Minhee Yun,et al.  Highly sensitive single polyaniline nanowire biosensor for the detection of immunoglobulin G and myoglobin. , 2011, Biosensors & bioelectronics.

[24]  Shazib Pervaiz,et al.  Comparative analysis of cardiac troponin i and creatine kinase‐MB as markers of acute myocardial infarction , 1997, Clinical cardiology.

[25]  S. Aldous,et al.  Cardiac biomarkers in acute myocardial infarction. , 2013, International journal of cardiology.

[26]  Adil Denizli,et al.  Microcontact imprinted surface plasmon resonance sensor for myoglobin detection. , 2013, Materials science & engineering. C, Materials for biological applications.

[27]  M. Goreti F. Sales,et al.  Electrochemical biosensor based on biomimetic material for myoglobin detection , 2013 .

[28]  Andrey V Lisitsa,et al.  Highly sensitive detection of human cardiac myoglobin using a reverse sandwich immunoassay with a gold nanoparticle-enhanced surface plasmon resonance biosensor. , 2013, Analytica chimica acta.

[29]  Yun Xiang,et al.  Personal glucose sensor for point-of-care early cancer diagnosis. , 2012, Chemical communications.

[30]  Kemin Wang,et al.  Using personal uric acid meter and enzyme-DNA conjugate for portable and quantitative DNA detection , 2013 .

[31]  Yi Lu,et al.  Using commercially available personal glucose meters for portable quantification of DNA. , 2012, Analytical chemistry.

[32]  Aninda J. Bhattacharyya,et al.  Employing denaturation for rapid electrochemical detection of myoglobin using TiO2 nanotubes. , 2013, Journal of materials chemistry. B.