DEPTH INTENSITY CORRECTION OF BIOFILM VOLUME DATA FROM CONFOCAL LASER SCANNING MICROSCOPES

GSF-IBB,Guest Scientist from Stat. Math. Unit, Indian Statistical Institute,Calcutta, Indiae-mail:Karsten@Rodenacker.deABSTRACTFluorescent signal intensities from confocal laser scanning microscopes (CLSM) suffer from severaldistortions inherent to the method. Namely, these are the decay of intensities with increasingdepth by finite transparency of the media used, the effects of extinction of the examined objects,the bleaching of the fluorochrome and stray light at surfaces within the volume under research.Under certain assumptions the decay of intensities can be estimated and used for a partial depthintensity correction. This estimation of the approximated intensity decay function is described andits correction effect is outlined.Volume and local distribution parameters of bacterial cultures marked by fluorescent in situhybridization (FISH) are measured. Measurements with different corrections are compared withmeasurements of original data.Keywords: biofilm, confocal microscopy, depth correction.

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