Methods for measurement of antibody/antigen affinity based on ELISA and RIA.

Various enzyme-linked immunosorbent assay or radioimmunoassay methods are currently used to quantify the antibody-antigen interaction. Only those based on indirect competition--enzyme-linked immunosorbent assay or radioimmunoassay--can provide the real thermodynamic affinity of the antibody for its antigen. They can be applied to a variety of experimental situations, some of which are reviewed here.

[1]  M. Steward,et al.  An inhibition enzyme immunoassay for estimating relative antibody affinity and affinity heterogeneity. , 1988, Journal of immunological methods.

[2]  A. Skretting,et al.  Evaluation of labelled monoclonal antibodies by simultaneous estimation of the association constant, the immunoreactive fraction, and the number of effective binding sites on the specific target. , 1992, Journal of immunological methods.

[3]  D R Burton,et al.  A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. Butler,et al.  The physical and functional behavior of capture antibodies adsorbed on polystyrene. , 1992, Journal of immunological methods.

[5]  S. Smith‐Gill,et al.  Experimental analysis by site-directed mutagenesis of somatic mutation effects on affinity and fine specificity in antibodies specific for lysozyme. , 1992, Journal of immunology.

[6]  J. G. Michael,et al.  Artifacts and limitations of enzyme immunoassay. , 1992, Journal of immunological methods.

[7]  G. Long,et al.  Binding of protein S to C4b-binding protein. Mutagenesis of protein S. , 1992, The Journal of biological chemistry.

[8]  J. Barbet,et al.  Statistical properties of immunoanalytic system. , 1992, Journal of immunological methods.

[9]  J. Butler,et al.  The immunochemistry of sandwich ELISAs--V. The capture antibody performance of polyclonal antibody-enriched fractions prepared by various methods. , 1992, Molecular immunology.

[10]  H. Bosshard,et al.  Caveats for the use of surface-adsorbed protein antigen to test the specificity of antibodies. , 1992, Journal of immunological methods.

[11]  V. Pascual,et al.  Clonally related IgM rheumatoid factors undergo affinity maturation in the rheumatoid synovial tissue. , 1992, Journal of immunology.

[12]  B. Nall,et al.  Diffusion-limited rates for monoclonal antibody binding to cytochrome c. , 1992, Biochemistry.

[13]  A. Lang,et al.  Analyses of affinity distributions within polyclonal populations of antigen-specific antibodies. Evaluation of their accuracy in population detection using monoclonal antibodies. , 1992, Journal of immunological methods.

[14]  E. Weiss,et al.  Measurement of affinity of viral monoclonal antibodies using Fab'-peroxidase conjugate. Influence of antibody concentration on apparent affinity. , 1992, Molecular immunology.

[15]  D. Landais,et al.  Design of yeast-secreted albumin derivatives for human therapy: biological and antiviral properties of a serum albumin-CD4 genetic conjugate. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[16]  B. Friguet,et al.  Conformational effects of ligand binding on the beta 2 subunit of Escherichia coli tryptophan synthase analyzed with monoclonal antibodies. , 1986, Biochemistry.

[17]  M. Goldberg.  Investigating protein conformation, dynamics and folding with monoclonal antibodies. , 1991, Trends in biochemical sciences.

[18]  D. Winzor,et al.  Adaptation of the Müller method to allow quantitative characterization of the affinity and cross-reactivity of antibodies by competitive radioimmunoassay. , 1991, Molecular immunology.

[19]  J. Beatty,et al.  Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. , 1987, Journal of immunological methods.

[20]  T. Ternynck,et al.  Natural mouse IgG reacts with self antigens including molecules involved in the immune response , 1992, European journal of immunology.

[21]  F. Stevens,et al.  Modification of an ELISA-based procedure for affinity determination: correction necessary for use with bivalent antibody. , 1987, Molecular immunology.

[22]  A. Chaffotte,et al.  Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. , 1985, Journal of immunological methods.

[23]  L. Presta,et al.  Humanization of an anti-p185HER2 antibody for human cancer therapy. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[24]  A. Nieto,et al.  Direct measurement of antibody affinity distribution by hapten-inhibition enzyme immunoassay. , 1984, Molecular immunology.

[25]  B. Friguet,et al.  Folding on the ribosome of Escherichia coli tryptophan synthase beta subunit nascent chains probed with a conformation-dependent monoclonal antibody. , 1992, Journal of molecular biology.