Track structure, lesion development, and cell survival.

A stochastic track-structure-dependent model is presented based on DNA double-strand breaks (DSBs) interacting in a time- and distance-dependent manner, and in competition with DSB repair, to form exchange-type chromosomal aberrations. Many models of cell survival involve estimation of mean numbers of lesions per cell, which is then related to cell survival. Unless this relationship is linear, this implies that a cell responds not to the number of lesions produced in it, but to the mean number of lesions in all the exposed cells; this is clearly unrealistic, particularly for phenomena such as saturation. In contrast to such deterministic approaches, we describe a stochastic model, in which individual cells are considered and exposed to Monte Carlo-generated tracks of various radiations. The elementary sublesions produced (DSBs) diffuse, repair, or interact, forming lesions (chromosomal exchange-type aberrations) in a time- and distance-dependent manner. Results agree well with experiments for survival of synchronous Chinese hamster V-79 cells exposed to X rays and radiations with LETs from 20 to 170 keV/microns. Thus the main features of survival for low-, medium-, and high-LET radiation are understandable in terms of a single approach, the relative responses to different radiations being determined by their different energy deposition patterns.

[1]  A. Kellerer,et al.  A Generalized Formulation of Dual Radiation Action1 , 1978 .

[2]  M. Zaider,et al.  On the stochastic treatment of fast chemical reactions. , 1984, Radiation research.

[3]  J. Thacker,et al.  Mutation and inactivation of cultured mammalian cells exposed to beams of accelerated heavy ions. II. Chinese hamster V79 cells. , 1979, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[4]  D. Goodhead,et al.  Estimation of a single property of low LET radiations which correlates with biological effectiveness. , 1983, Physics in medicine and biology.

[5]  K. F. Weibezahn,et al.  Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes , 1981, Nucleic Acids Res..

[6]  J. Ward,et al.  Biochemistry of DNA lesions. , 1985, Radiation research. Supplement.

[7]  J. Turner,et al.  Monte Carlo calculation of initial energies of Compton electrons and photoelectrons in water irradiated by photons with energies up to 2 MeV. , 1980, Health physics.

[8]  E. Bothe,et al.  Single- and double-strand break formation in DNA irradiated in aqueous solution: dependence on dose and OH radical scavenger concentration. , 1987, Radiation research.

[9]  Marco Zaider,et al.  The Applications of Track Calculations to Radiobiology I. Monte Carlo Simulation of Proton Tracks , 1983 .

[10]  H. E. Johns,et al.  Physics of Radiology , 1983 .

[11]  P. W. Hochachka.  The Role of Oxygen Oxygen and Living Processes: An Interdisciplinary Approach Daniel Gilbert , 1982 .

[12]  Albrecht M. Kellerer,et al.  THEORY OF DUAL RADIATION ACTION. , 1972 .

[13]  M. Zaider,et al.  Modification of the theory of dual radiation action for attenuated fields. II. Application to the analysis of soft X-ray results. , 1984, Radiation research.

[14]  J. Warman,et al.  A positron annihilation study of hydrated DNA , 1986, Biopolymers.

[15]  M. Zaider,et al.  Stochastic and Deterministic Treatments of the Time Decay of Species Created by Heavy Charged Particle Interactions , 1985 .

[16]  S B Curtis,et al.  Lethal and potentially lethal lesions induced by radiation--a unified repair model. , 1986, Radiation research.

[17]  M. Bender,et al.  Radiation-induced cellular reproductive death and chromosome aberrations. , 1978, Radiation research.

[18]  M. Zaider,et al.  Modification of the theory of dual radiation action for attenuated fields. I. Basic formalism. , 1984, Radiation research.

[19]  M. Zaider,et al.  Inactivation of synchronized mammalian cells with low-energy X rays--results and significance. , 1987, Radiation research.

[20]  P. Cullis,et al.  Effects of ionizing radiation on deoxyribonucleic acid and related systems. Part 1. The rôle of oxygen , 1984 .

[21]  D. Pearson,et al.  V79 survival following simultaneous or sequential irradiation by 15-MeV neutrons and 60Co photons. , 1983, Radiation Research.

[22]  M. Zaider,et al.  The application of track calculations to radiobiology. III. Analysis of the molecular beam experiment results. , 1984, Radiation research.

[23]  A. Carrano.  Chromosome aberrations and radiation-induced cell death. II. Predicted and observed cell survival. , 1973, Mutation research.

[24]  S. H. Revell.  The breakage-and-reunion theory and the exchange theory for chromosomal aberrations induced by ionizing radiations: a short history. , 1974 .

[25]  M S Meselson,et al.  A general model for genetic recombination. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[26]  D. Goodhead,et al.  Radiobiology of ultrasoft X rays. I. Cultured hamster cells (V79). , 1987, Radiation research.

[27]  D T Goodhead,et al.  Effectiveness of 0.3 keV carbon ultrasoft X-rays for the inactivation and mutation of cultured mammalian cells. , 1979, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[28]  M. Zaider,et al.  Comments on "V79 survival following simultaneous or sequential irradiation by 15-MeV neutrons and 60Co photons". , 1984, Radiation research.

[29]  K. Sax.  An Analysis of X-Ray Induced Chromosomal Aberrations in Tradescantia. , 1940, Genetics.

[30]  R. Okayasu,et al.  Linear DNA elution dose response curves obtained in CHO cells with non-unwinding filter elution after appropriate selection of the lysis conditions. , 1989, International journal of radiation biology.

[31]  S. Chandrasekhar.  Stochastic problems in Physics and Astronomy , 1943 .

[32]  M. Zaider,et al.  Saturation in Dual Radiation Action , 1988 .

[33]  E. Hall,et al.  The repair of sublethal damage in diploid human fibroblasts: a comparison between human and rodent cell lines. , 1983, Radiation research.

[34]  C. Geard,et al.  Inactivation of synchronized Chinese Hamster V79 cells with charged-particle track segments. , 1980, Radiation research.