blaOXA -731 , a new chromosome-encoded blaOXA -48 -like variant in Shewanella sp. from the aquatic environment in Myanmar.

Shewanella sp., the progenitors of blaOXA-48 -like genes are increasingly reported with the possession of different blaOXA-48 -like variants. This study aims to characterize blaOXA-731 , a new variant of blaOXA-48 -like gene identified in Shewanella sp. isolated from the aquatic environment in Myanmar. Phylogenetic analysis of the blaOXA-731 sequence with other blaOXA-48 -like variants showed that it has the highest nucleotide identity of 86.09% with blaOXA-48 . However, the active site motifs in OXA-731 were 100% identical to that in OXA-48. Whole genome sequencing analysis showed that blaOXA-731 is not surrounded by any mobile genetic elements. The genetic context of blaOXA-731 was found as similar to other blaOXA-48 -like genes previously identified in Shewanella sp. S1 nuclease pulsed-field gel electrophoresis followed by Southern blotting confirmed the location of blaOXA-731 in the chromosome of Shewanella genome. Cloning and expression studies showed that OXA-731 has β-lactamase activity similar to OXA-48 and OXA-181, but it has no significant carbapenemase activity. Our results showed the significance of blaOXA-48 -like-carrying Shewanella sp. in the spreading of blaOXA-48 -like genes in the community. This article is protected by copyright. All rights reserved.

[1]  Yogendra Pratap Singh,et al.  Detection of Pan drug resistance OXA-48 producing Providencia in an ICU patient for the first time in Nepal , 2019, Antimicrobial Resistance & Infection Control.

[2]  S. Hamada,et al.  Dissemination of carbapenemase-producing Enterobacteriaceae harbouring blaNDM or blaIMI in local market foods of Yangon, Myanmar , 2019, Scientific Reports.

[3]  V. Kumar,et al.  Structural Analysis of The OXA-48 Carbapenemase Bound to A “Poor” Carbapenem Substrate, Doripenem , 2019, Antibiotics.

[4]  Rathina Kumar Shanmugakani,et al.  Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar , 2018, Antimicrobial Agents and Chemotherapy.

[5]  E. Peterson,et al.  Antibiotic Resistance Mechanisms in Bacteria: Relationships Between Resistance Determinants of Antibiotic Producers, Environmental Bacteria, and Clinical Pathogens , 2018, Front. Microbiol..

[6]  A. Sumi,et al.  Prevalence of Extended-Spectrum Beta-Lactamase and Carbapenemase Genes in Clinical Isolates of Escherichia coli in Myanmar: Dominance of blaNDM-5 and Emergence of blaOXA-181. , 2018, Microbial drug resistance.

[7]  T. Naas,et al.  Molecular Characterization of OXA-198 Carbapenemase-Producing Pseudomonas aeruginosa Clinical Isolates , 2018, Antimicrobial Agents and Chemotherapy.

[8]  J. Rolain,et al.  Characterisation of blaOXA-538, a new variant of blaOXA-48, in Shewanella xiamenensis isolated from river water in Algeria. , 2017, Journal of global antimicrobial resistance.

[9]  A. Alves,et al.  Shewanella species as the origin of blaOXA-48 genes: insights into gene diversity, associated phenotypes and possible transfer mechanisms. , 2017, International journal of antimicrobial agents.

[10]  T. Naas,et al.  CTX-M-15-Producing Shewanella Species Clinical Isolate Expressing OXA-535, a Chromosome-Encoded OXA-48 Variant, Putative Progenitor of the Plasmid-Encoded OXA-436 , 2017, Antimicrobial Agents and Chemotherapy.

[11]  J. Crump,et al.  ESBL- and Carbapenemase-Producing Enterobacteriaceae in Patients with Bacteremia, Yangon, Myanmar, 2014 , 2017, Emerging infectious diseases.

[12]  Rathina Kumar Shanmugakani,et al.  PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens , 2017, Antimicrobial Agents and Chemotherapy.

[13]  Rathina Kumar Shanmugakani,et al.  Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens , 2017, BMC Infectious Diseases.

[14]  D. Ceccarelli,et al.  Chromosome-Based blaOXA-48-Like Variants in Shewanella Species Isolates from Food-Producing Animals, Fish, and the Aquatic Environment , 2016, Antimicrobial Agents and Chemotherapy.

[15]  A. Singer,et al.  Review of Antimicrobial Resistance in the Environment and Its Relevance to Environmental Regulators , 2016, Front. Microbiol..

[16]  G. Meletis,et al.  Carbapenem resistance: overview of the problem and future perspectives , 2016, Therapeutic advances in infectious disease.

[17]  J. Rolain,et al.  Emergence of Carbapenem-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Clinical Isolates Collected from Some Libyan Hospitals. , 2015, Microbial drug resistance.

[18]  P. Nordmann,et al.  Genetic and Biochemical Characterization of OXA-405, an OXA-48-Type Extended-Spectrum β-Lactamase without Significant Carbapenemase Activity , 2015, Antimicrobial Agents and Chemotherapy.

[19]  S. Amyes,et al.  OXA β-Lactamases , 2014, Clinical Microbiology Reviews.

[20]  G. Feierl,et al.  KPC-2 and OXA-48 carbapenemase-harbouring Enterobacteriaceae detected in an Austrian wastewater treatment plant. , 2014, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[21]  Gerard D. Wright,et al.  Intrinsic antibiotic resistance: mechanisms, origins, challenges and solutions. , 2013, International journal of medical microbiology : IJMM.

[22]  A. Petroni,et al.  Intrapatient emergence of OXA-247: a novel carbapenemase found in a patient previously infected with OXA-163-producing Klebsiella pneumoniae. , 2013, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[23]  Z. Zong.  Discovery of bla OXA-199, a Chromosome-Based bla OXA-48-Like Variant, in Shewanella xiamenensis , 2012, PloS one.

[24]  P. Nordmann,et al.  OXA-48-like carbapenemases: the phantom menace. , 2012, The Journal of antimicrobial chemotherapy.

[25]  T. Berendonk,et al.  Origin and Evolution of Antibiotic Resistance: The Common Mechanisms of Emergence and Spread in Water Bodies , 2011, Front. Microbio..

[26]  P. Nordmann,et al.  Occurrence of the Carbapenem-Hydrolyzing β-Lactamase Gene blaOXA-48 in the Environment in Morocco , 2011, Antimicrobial Agents and Chemotherapy.

[27]  P. Nordmann,et al.  Origin of OXA-181, an Emerging Carbapenem-Hydrolyzing Oxacillinase, as a Chromosomal Gene in Shewanella xiamenensis , 2011, Antimicrobial Agents and Chemotherapy.

[28]  Ronald N. Jones,et al.  OXA-163, an OXA-48-Related Class D β-Lactamase with Extended Activity Toward Expanded-Spectrum Cephalosporins , 2011, Antimicrobial Agents and Chemotherapy.

[29]  F. Baquero,et al.  Antibiotics and antibiotic resistance in water environments. , 2008, Current opinion in biotechnology.

[30]  Jean-Michel Claverie,et al.  Phylogeny.fr: robust phylogenetic analysis for the non-specialist , 2008, Nucleic Acids Res..

[31]  K. Bush,et al.  Carbapenemases: the Versatile β-Lactamases , 2007, Clinical Microbiology Reviews.

[32]  P. Nordmann,et al.  Emergence of Oxacillinase-Mediated Resistance to Imipenem in Klebsiella pneumoniae , 2004, Antimicrobial Agents and Chemotherapy.