‘Omics’ Tools in Soil Microbiology: The State of the Art

The soil being the most heterogeneous substance hosts the dynamic environments for diverse microorganisms. Traditional techniques are limited to explore only few portion of massive unknown soil microbial world due to their well-known biasness in detecting microbial genetics and functional diversity. With this respect, omics targets the powerful genomics, metagenomics, transcriptomics, proteomics and metabolomic tools to explore the vast microbial community, new biomolecules and novel pathways. It helps to better understand the toxicity mechanisms, predicts the risks associated with environmental toxicity and aids in bioprospecting of value-added products. These new approaches will be useful to establish the linkage between structure and function of soil microbial community and help to get better insight of the ecological processes in the environment with special emphasis on plant-microbe ecosystems. The present chapter will give an overview of the application of the advanced molecular tools as well as their potentials and limitations in studying the soil microbial ecology.

[1]  S. Mocali,et al.  Exploring research frontiers in microbiology: the challenge of metagenomics in soil microbiology. , 2010, Research in microbiology.

[2]  Mark R. Viant,et al.  Applications of metabolomics to the environmental sciences , 2009, Metabolomics.

[3]  C. Sensen,et al.  First insight into the genome of an uncultivated crenarchaeote from soil. , 2002, Environmental microbiology.

[4]  E. Kandeler,et al.  Microbial Population Structures in Soil Particle Size Fractions of a Long-Term Fertilizer Field Experiment , 2001, Applied and Environmental Microbiology.

[5]  W. König,et al.  Genetic Classification and Distinguishing of Staphylococcus Species Based on Different Partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf Gene Sequences , 2008, Journal of Clinical Microbiology.

[6]  B. Roschitzki,et al.  Community proteogenomics reveals insights into the physiology of phyllosphere bacteria , 2009, Proceedings of the National Academy of Sciences.

[7]  M. F. Noronha,et al.  Potential of semiarid soil from Caatinga biome as a novel source for mining lignocellulose-degrading enzymes. , 2017, FEMS microbiology ecology.

[8]  M. Mann,et al.  A proteomic fingerprint of dissolved organic carbon and of soil particles , 2004, Oecologia.

[9]  M. Hungria,et al.  Genetic Characterization of Soybean Rhizobia in Paraguay , 2000, Applied and Environmental Microbiology.

[10]  P. Hugenholtz Exploring prokaryotic diversity in the genomic era , 2002, Genome Biology.

[11]  Emma L. Schymanski,et al.  Mass spectral databases for LC/MS- and GC/MS-based metabolomics: state of the field and future prospects , 2016 .

[12]  S. Tringe,et al.  Comparative Metagenomics of Microbial Communities , 2004, Science.

[13]  M. Moran Metatranscriptomics: Eavesdropping on Complex Microbial Communities , 2009 .

[14]  Y. Choi,et al.  Metabolic response of tomato leaves upon different plant-pathogen interactions. , 2010, Phytochemical analysis : PCA.

[15]  L. Fraissinet-Tachet,et al.  Metatranscriptomics Reveals the Diversity of Genes Expressed by Eukaryotes in Forest Soils , 2012, PloS one.

[16]  Erin B. Taylor,et al.  Metaproteomic characterization of a soil microbial community following carbon amendment , 2010 .

[17]  R. B. Jackson,et al.  Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays , 2005, Applied and Environmental Microbiology.

[18]  P. Schenk,et al.  Unraveling plant-microbe interactions: can multi-species transcriptomics help? , 2012, Trends in biotechnology.

[19]  Dazhi Wang,et al.  Environmental Microbial Community Proteomics: Status, Challenges and Perspectives , 2016, International journal of molecular sciences.

[20]  N. Thomson,et al.  Studying bacterial transcriptomes using RNA-seq , 2010, Current opinion in microbiology.

[21]  A. Hayes,et al.  Transcriptional response of Pseudomonas aeruginosa to a phosphate-deficient Lolium perenne rhizosphere , 2011, Plant and Soil.

[22]  F. Bastida,et al.  Metaproteomics of soils from semiarid environment: functional and phylogenetic information obtained with different protein extraction methods. , 2014, Journal of proteomics.

[23]  M. Fedurco,et al.  BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies , 2006, Nucleic acids research.

[24]  J. Jansson,et al.  The Potential of Metagenomic Approaches for Understanding Soil Microbial Processes , 2014 .

[25]  E. Wellington,et al.  Resolving functional diversity in relation to microbial community structure in soil: exploiting genomics and stable isotope probing. , 2003, Current opinion in microbiology.

[26]  J. Handelsman,et al.  Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms , 2000, Applied and Environmental Microbiology.

[27]  B. Montanini,et al.  A metaproteomic approach dissecting major bacterial functions in the rhizosphere of plants living in serpentine soil , 2017, Analytical and Bioanalytical Chemistry.

[28]  J. S. Sinninghe Damsté,et al.  Impact of Seasonal Hypoxia on Activity and Community Structure of Chemolithoautotrophic Bacteria in a Coastal Sediment , 2017, Applied and Environmental Microbiology.

[29]  Gerald T Ankley,et al.  Toxicogenomics in regulatory ecotoxicology. , 2006, Environmental science & technology.

[30]  R. Margis,et al.  Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries , 2012, Genetics and molecular biology.

[31]  J. Kur,et al.  Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library. , 2009, Polish journal of microbiology.

[32]  J. Tiedje,et al.  Advantages of the metagenomic approach for soil exploration: reply from Vogel et al. , 2009, Nature Reviews Microbiology.

[33]  J. Handelsman,et al.  Metagenomics: genomic analysis of microbial communities. , 2004, Annual review of genetics.

[34]  H. Bouwmeester,et al.  Metabolomics in the Rhizosphere: Tapping into Belowground Chemical Communication. , 2016, Trends in plant science.

[35]  F. Abram,et al.  Exploring mixed microbial community functioning: recent advances in metaproteomics , 2012, FEMS microbiology ecology.

[36]  R. Yadav,et al.  Metatranscriptomics of Soil Eukaryotic Communities. , 2016, Methods in molecular biology.

[37]  H. Insam Developments in soil microbiology since the mid 1960s , 2001 .

[38]  Soil solid phases effects on the proteomic analysis of Cupriavidus metallidurans CH34 , 2012, Biology and Fertility of Soils.

[39]  Seon-Woo Lee,et al.  Characterization of a Forest Soil Metagenome Clone That Confers Indirubin and Indigo Production on Escherichia coli , 2005, Applied and Environmental Microbiology.

[40]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[41]  Y. Choi,et al.  Metal ion-inducing metabolite accumulation in Brassica rapa. , 2008, Journal of plant physiology.

[42]  Diego A. A. Chaves,et al.  The Microbiome of Brazilian Mangrove Sediments as Revealed by Metagenomics , 2012, PloS one.

[43]  Daniel H. Huson,et al.  Simultaneous Assessment of Soil Microbial Community Structure and Function through Analysis of the Meta-Transcriptome , 2008, PloS one.

[44]  Oliver A.H. Jones,et al.  Metabolomic analysis of soil communities can be used for pollution assessment , 2014, Environmental toxicology and chemistry.

[45]  B. Moon,et al.  Production of porphyrin intermediates in Escherichia coli carrying soil metagenomic genes. , 2009, FEMS microbiology letters.

[46]  S D Allison,et al.  A trait-based approach for modelling microbial litter decomposition. , 2012, Ecology letters.

[47]  M. Wésolowski-Louvel,et al.  Soil eukaryotic functional diversity, a metatranscriptomic approach , 2007, The ISME Journal.

[48]  A. Becker,et al.  Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates , 2012, BMC Microbiology.

[49]  F. Gigliucci,et al.  Comparative analysis of metagenomes of Italian top soil improvers , 2017, Environmental research.

[50]  E. Topp,et al.  Characterization of an Atrazine-DegradingPseudaminobacter sp. Isolated from Canadian and French Agricultural Soils , 2000, Applied and Environmental Microbiology.

[51]  John M Eppley,et al.  Community transcriptomics reveals universal patterns of protein sequence conservation in natural microbial communities , 2011, Genome Biology.

[52]  J. Gilbert,et al.  Detection of Large Numbers of Novel Sequences in the Metatranscriptomes of Complex Marine Microbial Communities , 2008, PloS one.

[53]  Eoin L. Brodie,et al.  Direct cellular lysis/protein extraction protocol for soil metaproteomics. , 2010, Journal of proteome research.

[54]  Matthias Hess,et al.  A perspective: metatranscriptomics as a tool for the discovery of novel biocatalysts. , 2009, Journal of biotechnology.

[55]  W. Liesack,et al.  Short-Term Exposure of Paddy Soil Microbial Communities to Salt Stress Triggers Different Transcriptional Responses of Key Taxonomic Groups , 2017, Front. Microbiol..

[56]  S. Xie,et al.  Transcriptome profiling of Bacillus subtilis OKB105 in response to rice seedlings , 2015, BMC Microbiology.

[57]  A. Barsch,et al.  A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti , 2017, Metabolomics.

[58]  M. Firestone,et al.  Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses , 2013, The ISME Journal.

[59]  Sagar M. Utturkar,et al.  Enrichment of Root Endophytic Bacteria from Populus deltoides and Single-Cell-Genomics Analysis , 2016, Applied and Environmental Microbiology.

[60]  K. Konstantinidis,et al.  The bacterial species definition in the genomic era , 2006, Philosophical Transactions of the Royal Society B: Biological Sciences.

[61]  W. Liesack,et al.  Identification of microbial populations driving biopolymer degradation in acidic peatlands by metatranscriptomic analysis , 2016, Molecular ecology.

[62]  Christian Gieger,et al.  Genetics Meets Metabolomics: A Genome-Wide Association Study of Metabolite Profiles in Human Serum , 2008, PLoS genetics.

[63]  S. Tringe,et al.  Tackling soil diversity with the assembly of large, complex metagenomes , 2014, Proceedings of the National Academy of Sciences.

[64]  M. Strous,et al.  Assessing species biomass contributions in microbial communities via metaproteomics , 2017, Nature Communications.

[65]  P. Gallusci,et al.  Effects of long-term cadmium exposure on growth and metabolomic profile of tomato plants. , 2010, Ecotoxicology and environmental safety.

[66]  Simone Rochfort,et al.  Metabolomics reviewed: a new "omics" platform technology for systems biology and implications for natural products research. , 2005, Journal of natural products.

[67]  Y. Choi,et al.  Metabolomic analysis of methyl jasmonate treated Brassica rapa leaves by 2-dimensional NMR spectroscopy. , 2006, Phytochemistry.

[68]  Tom O. Delmont,et al.  Accessing the Soil Metagenome for Studies of Microbial Diversity , 2010, Applied and Environmental Microbiology.

[69]  Jizhong Zhou,et al.  Phylogenetic Molecular Ecological Network of Soil Microbial Communities in Response to Elevated CO2 , 2011, mBio.

[70]  T. Heulin,et al.  Colonization of Wheat Roots by an Exopolysaccharide-ProducingPantoea agglomerans Strain and Its Effect on Rhizosphere Soil Aggregation , 1998, Applied and Environmental Microbiology.

[71]  Sagar M. Utturkar,et al.  Draft Genome Sequence for Caulobacter sp. Strain OR37, a Bacterium Tolerant to Heavy Metals , 2013, Genome Announcements.

[72]  A. Franks,et al.  Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[73]  Y. Choi,et al.  Metabolomic response of Brassica rapa submitted to pre-harvest bacterial contamination , 2008 .

[74]  H. Itoh,et al.  Predominant but Previously-overlooked Prokaryotic Drivers of Reductive Nitrogen Transformation in Paddy Soils, Revealed by Metatranscriptomics , 2017, Microbes and environments.

[75]  F. Bastida,et al.  Soil metaproteomics: a review of an emerging environmental science. Significance, methodology and perspectives , 2009 .

[76]  T. Urich,et al.  The “Double‐RNA” Approach to Simultaneously Assess the Structure and Function of a Soil Microbial Community , 2011 .

[77]  J. Tokuhisa,et al.  Metabolite profiling of Arabidopsis seedlings in response to exogenous sinalbin and sulfur deficiency. , 2011, Phytochemistry.

[78]  Jean-Marc Nuzillard,et al.  NMR metabolomics to revisit the tobacco mosaic virus infection in Nicotiana tabacum leaves. , 2006, Journal of natural products.

[79]  Sheng Lin,et al.  Metaproteomic analysis of ratoon sugarcane rhizospheric soil , 2013, BMC Microbiology.

[80]  R. Daniel,et al.  Identification of novel lipolytic genes and gene families by screening of metagenomic libraries derived from soil samples of the German Biodiversity Exploratories. , 2011, FEMS microbiology ecology.

[81]  Robert Hettich,et al.  Environmental Proteomics: a Paradigm Shift in Characterizing Microbial Activities at the Molecular Level , 2009, Microbiology and Molecular Biology Reviews.

[82]  Jürgen Eck,et al.  Acidobacteria form a coherent but highly diverse group within the bacterial domain: evidence from environmental genomics , 2003, Molecular microbiology.

[83]  Stephan Fuchs,et al.  Soil and leaf litter metaproteomics—a brief guideline from sampling to understanding , 2016, FEMS microbiology ecology.

[84]  Tong Zhang,et al.  Metagenomic assembly unravel microbial response to redox fluctuation in acid sulfate soil , 2017 .

[85]  E. Taylor,et al.  Microbial Protein in Soil: Influence of Extraction Method and C Amendment on Extraction and Recovery , 2010, Microbial Ecology.

[86]  C. Schleper,et al.  Genomic studies of uncultivated archaea , 2005, Nature Reviews Microbiology.

[87]  Paul Wilmes,et al.  Metaproteomics: studying functional gene expression in microbial ecosystems. , 2006, Trends in microbiology.

[88]  Marion G Miller,et al.  Environmental metabolomics: a SWOT analysis (strengths, weaknesses, opportunities, and threats). , 2007, Journal of proteome research.

[89]  P. Nannipieri Role of Stabilised Enzymes in Microbial Ecology and Enzyme Extraction from Soil with Potential Applications in Soil Proteomics , 2006 .

[90]  L. Øvreås,et al.  Microbial diversity and function in soil: from genes to ecosystems. , 2002, Current opinion in microbiology.

[91]  J. Banfield,et al.  Proteogenomic approaches for the molecular characterization of natural microbial communities. , 2005, Omics : a journal of integrative biology.

[92]  C. Schleper,et al.  Characterization of large-insert DNA libraries from soil for environmental genomic studies of Archaea. , 2004, Environmental microbiology.

[93]  L. Brussaard,et al.  Relationships between soil texture, physical protection of organic matter, soil biota, and c and n mineralization in grassland soils , 1993 .

[94]  Haibin Wang,et al.  Comparative Metaproteomic Analysis on Consecutively Rehmannia glutinosa-Monocultured Rhizosphere Soil , 2011, PloS one.

[95]  F. Bastida,et al.  Tracing changes in the microbial community of a hydrocarbon-polluted soil by culture-dependent proteomics. , 2010 .

[96]  P. Garbeva,et al.  Microbial diversity in soil: selection microbial populations by plant and soil type and implications for disease suppressiveness. , 2004, Annual review of phytopathology.

[97]  Jiping Chen,et al.  The responses of Arabidopsis thaliana to cadmium exposure explored via metabolite profiling. , 2010, Chemosphere.

[98]  Eoin L. Brodie,et al.  13C-Isotopomer-based metabolomics of microbial groups isolated from two forest soils , 2008, Metabolomics.

[99]  R. Castenholz,et al.  Biogeographic and Phylogenetic Diversity of Thermoacidophilic Cyanidiales in Yellowstone National Park, Japan, and New Zealand , 2008, Applied and Environmental Microbiology.

[100]  H. Trindade,et al.  Cowpea (Vigna unguiculata L. Walp.) Metabolomics: Osmoprotection as a Physiological Strategy for Drought Stress Resistance and Improved Yield , 2017, Front. Plant Sci..

[101]  J. Bandow,et al.  Simple discovery of bacterial biocatalysts from environmental samples through functional metaproteomics , 2017, Microbiome.

[102]  Peter Salamon,et al.  Metagenomic and Small-Subunit rRNA Analyses Reveal the Genetic Diversity of Bacteria, Archaea, Fungi, and Viruses in Soil , 2007, Applied and Environmental Microbiology.

[103]  D. Benndorf,et al.  Functional metaproteome analysis of protein extracts from contaminated soil and groundwater , 2007, The ISME Journal.

[104]  Etienne Yergeau,et al.  Metagenomic Analysis of the Bioremediation of Diesel-Contaminated Canadian High Arctic Soils , 2012, PloS one.

[105]  S. Claassens,et al.  Can a metabolomics-based approach be used as alternative to analyse fatty acid methyl esters from soil microbial communities? , 2016 .

[106]  Kelly P. Nevin,et al.  Metatranscriptomic Evidence for Direct Interspecies Electron Transfer between Geobacter and Methanothrix Species in Methanogenic Rice Paddy Soils , 2017, Applied and Environmental Microbiology.