Clinicopathological significance of stanniocalcin 2 gene expression in colorectal cancer

Laser microdissection (LMD) and microarray were used to identify genes associated with colorectal cancer. Stanniocalcin 2 (STC2) expression and clinicopathological significance in 139 clinical colorectal cancer samples were specifically investigated using real‐time quantitative reverse transcription‐polymerase chain reaction. A number of genes upregulated in colorectal cancer cells compared to normal colorectal epithelial cells were identified including STC2. STC2 gene expression in cancer tissue was higher than in corresponding normal colorectal epithelial tissue in 124 of 139 cases (89.2%, p < 0.01). Tumors with high STC2 expression showed higher frequencies of lymph node metastasis, lymphatic invasion, tumor depth, tumor size and AJCC Stage classification (p < 0.01). Patients with high STC2 expression also showed significantly worse overall survival rates than those with low STC2 expression (p < 0.01). Furthermore, STC2 gene appeared to be associated with colorectal cancer progression and may be a useful prognostic indicator for colorectal cancer. © 2009 UICC

[1]  G. Kristiansen,et al.  Identification of stanniocalcin 2 as prognostic marker in renal cell carcinoma. , 2009, European urology.

[2]  P. Heikkilä,et al.  Tumor dormancy: elevated expression of stanniocalcins in late relapsing breast cancer. , 2008, Cancer letters.

[3]  Sanda Raulic,et al.  Stanniocalcin 2 expression is regulated by hormone signalling and negatively affects breast cancer cell viability in vitro. , 2008, The Journal of endocrinology.

[4]  G. F. Wagner,et al.  Epigenetic and HIF-1 regulation of stanniocalcin-2 expression in human cancer cells. , 2008, Experimental cell research.

[5]  A. Jemal,et al.  Cancer Statistics, 2008 , 2008, CA: a cancer journal for clinicians.

[6]  K. Mimori,et al.  Efficient identification of a novel cancer/testis antigen for immunotherapy using three-step microarray analysis. , 2008, Cancer research.

[7]  K. Mimori,et al.  Identification of overexpressed genes in hepatocellular carcinoma, with special reference to ubiquitin‐conjugating enzyme E2C gene expression , 2007, International journal of cancer.

[8]  Jorge S Reis-Filho,et al.  Identification of NTN4, TRA1, and STC2 as Prognostic Markers in Breast Cancer in a Screen for Signal Sequence Encoding Proteins , 2007, Clinical Cancer Research.

[9]  M. Nelson,et al.  Profiling of selenomethionine responsive genes in colon cancer by microarray analysis , 2007, Cancer biology & therapy.

[10]  K. Mimori,et al.  BAC clones related to prognosis in patients with esophageal squamous carcinoma: an array comparative genomic hybridization study. , 2007, The oncologist.

[11]  George Coukos,et al.  Tumor vascular proteins as biomarkers in ovarian cancer. , 2007, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[12]  K. Mimori,et al.  Opa Interacting Protein 5 (OIP5) Is a Novel Cancer-testis Specific Gene in Gastric Cancer , 2007, Annals of Surgical Oncology.

[13]  Tatsuo Kanda,et al.  Fatty Acid Binding Protein 6 Is Overexpressed in Colorectal Cancer , 2006, Clinical Cancer Research.

[14]  Katsuhiko Yanaga,et al.  Clinical Significance of TROP2 Expression in Colorectal Cancer , 2006, Clinical Cancer Research.

[15]  T. Utsunomiya,et al.  Clinical Significance of Human Kallikrein Gene 6 Messenger RNA Expression in Colorectal Cancer , 2005, Clinical Cancer Research.

[16]  T. Hanai,et al.  Global analysis of altered gene expressions during the process of esophageal squamous cell carcinogenesis in the rat: a study combined with a laser microdissection and a cDNA microarray. , 2005, Cancer research.

[17]  Shinzaburo Noguchi,et al.  mRNA expression level of estrogen‐inducible gene, α1‐antichymotrypsin, is a predictor of early tumor recurrence in patients with invasive breast cancers , 2004, Cancer science.

[18]  A. Fienberg,et al.  Characterization of Stanniocalcin 2, a Novel Target of the Mammalian Unfolded Protein Response with Cytoprotective Properties , 2004, Neurobiology of Aging.

[19]  R. Reddel,et al.  Mammalian stanniocalcins and cancer. , 2003, Endocrine-related cancer.

[20]  R. Kaufman,et al.  The unfolded protein response , 2003, Journal of Cell Science.

[21]  K. Mimori,et al.  Prognostic score of gastric cancer determined by cDNA microarray. , 2002, Clinical cancer research : an official journal of the American Association for Cancer Research.

[22]  Franklin Peale,et al.  In silico data filtering to identify new angiogenesis targets from a large in vitro gene profiling data set. , 2002, Physiological genomics.

[23]  M. Ichihara,et al.  Characterization of gene expression induced by RET with MEN2A or MEN2B mutation. , 2002, The American journal of pathology.

[24]  M. King,et al.  BRCA1 transcriptionally regulates genes involved in breast tumorigenesis , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[25]  K. Ishibashi,et al.  Prospect of a stanniocalcin endocrine/paracrine system in mammals. , 2002, American journal of physiology. Renal physiology.

[26]  Melissa C Southey,et al.  Stanniocalcin 2 is an estrogen-responsive gene coexpressed with the estrogen receptor in human breast cancer. , 2002, Cancer research.

[27]  T. Tsunoda,et al.  Genome-wide analysis of gene expression in human hepatocellular carcinomas using cDNA microarray: identification of genes involved in viral carcinogenesis and tumor progression. , 2001, Cancer research.

[28]  R. L. Baldwin,et al.  Differential gene expression between normal and tumor-derived ovarian epithelial cells. , 2000, Cancer research.

[29]  M. Larsen,et al.  Stanniocalcin 1 and 2 are secreted as phosphoproteins from human fibrosarcoma cells. , 2000, The Biochemical journal.

[30]  F. Peale,et al.  Gene expression profiling in an in vitro model of angiogenesis. , 2000, The American journal of pathology.

[31]  M. Monden,et al.  Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers. , 2000, International journal of oncology.

[32]  D. Thompson,et al.  Stanniocalcin 2: Characterization of the Protein and its Localization to Human Pancreatic Alpha Cells , 1999, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme.

[33]  K. White,et al.  Chromosomal Localization of Two Human Genes Involved in Phosphate Homeostasis: The Type IIb Sodium-Phosphate Cotransporter and Stanniocalcin-2 , 1998, Somatic cell and molecular genetics.

[34]  S. Sasaki,et al.  Molecular cloning of a second human stanniocalcin homologue (STC2). , 1998, Biochemical and biophysical research communications.

[35]  R. Reddel,et al.  Identification of a second stanniocalcin cDNA in mouse and human: Stanniocalcin 2 , 1998, Molecular and Cellular Endocrinology.

[36]  Robert F. Bonner,et al.  Laser Capture Microdissection: Molecular Analysis of Tissue , 1997, Science.

[37]  G. F. Wagner,et al.  Human stanniocalcin-2 exhibits potent growth-suppressive properties in transgenic mice independently of growth hormone and IGFs. , 2005, American journal of physiology. Endocrinology and metabolism.

[38]  Keizo Sugimachi,et al.  Analysis of the gene-expression profile regarding the progression of human gastric carcinoma. , 2002, Surgery.

[39]  F. Urano,et al.  Transcriptional and translational control in the Mammalian unfolded protein response. , 2002, Annual review of cell and developmental biology.

[40]  N. Dubrawsky Cancer statistics , 1989, CA: a cancer journal for clinicians.