Target cell-specific determinants of membrane fusion within the human immunodeficiency virus type 1 gp120 third variable region and gp41 amino terminus

The entry of human immunodeficiency virus type 1 (HIV-1) into target cells involves binding to the viral receptor (CD4) and membrane fusion events, the latter influenced by target cell factors other than CD4. The third variable (V3) region of the HIV-1 gp120 exterior envelope glycoprotein and the amino terminus of the HIV-1 gp41 transmembrane envelope glycoprotein have been shown to be important for the membrane fusion process. Here we demonstrate that some HIV-1 envelope glycoproteins containing an altered V3 region or gp41 amino terminus exhibit qualitatively different abilities to mediate syncytium formation and virus entry when different target cells are used. These results demonstrate that the structure of these HIV-1 envelope glycoprotein regions determines the efficiency of membrane fusion in a target cell-specific manner and support a model in which the gp41 amino terminus interacts directly or indirectly with the target cell during virus entry.

[1]  B. Cullen,et al.  Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1. , 1991, Science.

[2]  J. Morris,et al.  Alteration of HIV-1 infectivity and neutralization by a single amino acid replacement in the V3 loop domain. , 1991, AIDS research and human retroviruses.

[3]  H. Robinson,et al.  Human immunodeficiency virus type 1 NL4-3 replication in four T-cell lines: rate and efficiency of entry, a major determinant of permissiveness , 1991, Journal of virology.

[4]  T. Hideshima,et al.  An IgG human monoclonal antibody that reacts with HIV-1/GP120, inhibits virus binding to cells, and neutralizes infection. , 1991, Journal of immunology.

[5]  H. Gendelman,et al.  Identification of a determinant within the human immunodeficiency virus 1 surface envelope glycoprotein critical for productive infection of primary monocytes. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[6]  M. Akutsu,et al.  Host range mutant of human immunodeficiency virus type 1: modification of cell tropism by a single point mutation at the neutralization epitope in the env gene , 1991, Journal of virology.

[7]  J. Culp,et al.  Envelope glycoproteins from biologically diverse isolates of immunodeficiency viruses have widely different affinities for CD4. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[8]  C. Cheng‐Mayer,et al.  Macrophage and T cell-line tropisms of HIV-1 are determined by specific regions of the envelope gp!20 gene , 1991, Nature.

[9]  E. Freed,et al.  Identification of the principal neutralizing determinant of human immunodeficiency virus type 1 as a fusion domain , 1991, Journal of virology.

[10]  J. Sodroski,et al.  Attenuation of human immunodeficiency virus type 1 cytopathic effect by a mutation affecting the transmembrane envelope glycoprotein , 1991, Journal of virology.

[11]  W. O'brien,et al.  HIV-1 tropism for mononuclear phagocytes can be determined by regions of gp120 outside the CD4-binding domain , 1990, Nature.

[12]  E. Freed,et al.  Characterization of the fusion domain of the human immunodeficiency virus type 1 envelope glycoprotein gp41. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[13]  B. Moss,et al.  Human immunodeficiency virus envelope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells , 1990, Journal of virology.

[14]  B. Chesebro,et al.  Failure of human immunodeficiency virus entry and infection in CD4-positive human brain and skin cells , 1990, Journal of virology.

[15]  P. Earl,et al.  Identification of the fusion peptide of primate immunodeficiency viruses. , 1989, Science.

[16]  Lange,et al.  Evidence for a role of virulent human immunodeficiency virus (HIV) variants in the pathogenesis of acquired immunodeficiency syndrome: studies on sequential HIV isolates , 1989, Journal of virology.

[17]  J. V. van Dongen,et al.  Human immunodeficiency virus infection studied in CD4-expressing human-murine T-cell hybrids. , 1989, Virology.

[18]  S. Ohnishi.  Chapter 9 Fusion of Viral Envelopes with Cellular Membranes , 1988, Current Topics in Membranes and Transport.

[19]  J. Hoxie,et al.  Biological characterization of a simian immunodeficiency virus-like retrovirus (HTLV-IV): evidence for CD4-associated molecules required for infection , 1988, Journal of virology.

[20]  J. Skehel,et al.  Membrane fusion by peptide analogues of influenza virus haemagglutinin. , 1988, The Journal of general virology.

[21]  S. Matsushita,et al.  Characterization of a human immunodeficiency virus neutralizing monoclonal antibody and mapping of the neutralizing epitope , 1988, Journal of virology.

[22]  C. Cheng‐Mayer,et al.  Biologic features of HIV-1 that correlate with virulence in the host. , 1988, Science.

[23]  F. Booy,et al.  Effects of low pH on influenza virus. Activation and inactivation of the membrane fusion capacity of the hemagglutinin. , 1987, The Journal of biological chemistry.

[24]  G. Nakamura,et al.  Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptor , 1987, Cell.

[25]  Edgar G. Engleman,et al.  pH-independent HIV entry into CD4-positive T cells via virus envelope fusion to the plasma membrane , 1987, Cell.

[26]  A J Langlois,et al.  HTLV-III/LAV-neutralizing antibodies to an E. coli-produced fragment of the virus envelope. , 1986, Science.

[27]  R. Gallo,et al.  Restricted neutralization of divergent human T-lymphotropic virus type III isolates by antibodies to the major envelope glycoprotein. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[28]  Robin A. Weiss,et al.  The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain , 1986, Cell.

[29]  K. Steimer,et al.  Induction of CD4-dependent cell fusion by the HTLV-III/LAV envelope glycoprotein , 1986, Nature.

[30]  M. Gottlieb.  Immunologic aspects of the acquired immunodeficiency syndrome and male homosexuality. , 1986, The Medical clinics of North America.

[31]  J K Nicholson,et al.  Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule. , 1986, Science.

[32]  M. Gething,et al.  Studies on the mechanism of membrane fusion: site-specific mutagenesis of the hemagglutinin of influenza virus , 1986, The Journal of cell biology.

[33]  T. Waldmann,et al.  Qualitative analysis of immune function in patients with the acquired immunodeficiency syndrome. Evidence for a selective defect in soluble antigen recognition. , 1985, The New England journal of medicine.

[34]  R. Warnke,et al.  Monoclonal antibody and enzymatic profiles of human malignant T-lymphoid cells and derived cell lines. , 1984, Cancer research.

[35]  C. Griscelli,et al.  Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes. , 1984, Science.

[36]  B. Haynes,et al.  Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. , 1984, Science.

[37]  S. Salahuddin,et al.  Restricted expression of human T-cell leukemia--lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes. , 1983, Virology.

[38]  D. Mann,et al.  Isolation and transmission of human retrovirus (human t-cell leukemia virus). , 1983, Science.

[39]  I. Wilson,et al.  Changes in the conformation of influenza virus hemagglutinin at the pH optimum of virus-mediated membrane fusion. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[40]  B. Cullen.  Use of eukaryotic expression technology in the functional analysis of cloned genes. , 1987, Methods in enzymology.

[41]  J. Sodroski,et al.  Role of the HTLV-III/LAV envelope in syncytium formation and cytopathicity , 1986, Nature.

[42]  Luc Montagnier,et al.  T-lymphocyte T4 molecule behaves as the receptor for human retrovirus  LAV , 1984, Nature.

[43]  M. Greaves,et al.  The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus , 1984, Nature.