Mutations of p 53 Gene and Their Relation to Disease Progression in B-Cell Lymphoma

The alteration of p53 tumor suppressor gene was studied in 48 patients with 8-cell lymphoma. A sequential combined technique of polymerase chain reaction-mediated singlestrand conformational polymorphism (PCR-SSCP) or reverse transcription (RT)-PCR-SSCP and direct sequencing were used as a simple and sensitive approach to analyze nucleotide changes. By these methods, we identified 8 missense point mutations and 2 codon deletions in 9 of the 48 patients. These mutations were located in or close to the evolutionally

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