THE EFFECT OF RADIATION ON SPLENOMEGALY INDUCED BY THE FRIEND LEUKEMIA VIRUS AND ITS MODIFICATION BY ETHY-N-BIS(2,2-DIMETHYL-ETHYLAMIDINOPHOSPHORO)-CARBAMATE (AB-132), ACTINOMYCIN D, AND AET.

(1-11) for the assay of the effects of radiation in vivo with or without chemical substances that modify radiation response are based on the evaluation of bone marrow, hair root, thymus, testis, and spleen, on survival time, and on modification of the growth of transplanted tumors. The Friend leukemia virus2 (FLV) (12, 13) induces splenomegaly in appropriate host rodents. The proliferating cells in the spleen of infected mice respond homogeneously to radiation. The rate of enlargement of FLV spleen size depends on the period of time elapsed from the viral inoculation. A tenfold increase of the spleen size occurs in less than 2 weeks after viral inoculation. Laboratory isolation of the virus and its serial passage are easily performed, and the doses of whole-body irradiation required for the inhibition of the proliferating tumor do not affect the viability of the virus. Experiments to determine the value of FLV-induced splenomegaly as a useful tool for the determination of radiation effects are presented in this paper.

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