GRP23 plays a core role in E-type editosomes via interacting with MORFs and atypical PPR-DYWs in Arabidopsis mitochondria

Significance C-to-U RNA editing in plant organelles is carried out by a PPR-DYW protein or a protein complex that harnesses site recognition and deaminase activity. E-type PPR proteins can specify the editing site but lack the deaminase activity. This study shows that E-type PPR proteins specifically recruit trans deaminase MEF8/MEF8S, while GRP23, MORF1, and MORF8 facilitate this recruitment. In addition, some PPR-DYW proteins also need GRP23, presumably to recruit a second deaminase. This study reveals the functions of GRP23, MEF8/MEF8S, MORF1, and MORF8 and furthers our understanding of the RNA-editing mechanism in plant organelles.

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