Specific Inhibition of Plaque Formation to Phosphorylcholine by Antibody against Antibody

Spleen cells from BALB/c mice immunized with heat-killed rough pneumococci (strain R36A) or spleen cells from normal mice immunized in vitro with the same antigen produce direct hemolytic plaques against sheep erythrocytes coated with pneumococcal C polysaccharide or conjugated with phosphorylcholine. Formation of plaques is specifically inhibited by phosphorylcholine or by antiserum to mouse immunoglobulin A myeloma protein which binds phosphorylcholine. Thus, the myeloma proteins and normal BALB/c antibodies share similar idiotypic determinants. This experimental system is suitable for probing the role of the antigen receptor in the immune response.

[1]  A. Shimizu,et al.  Three Variable-Gene Pools common to IgM, IgG and IgA Immunoglobulins , 1970, Nature.

[2]  R. Dutton,et al.  Immunization of Normal Mouse Spleen Cell Suspensions in vitro , 1966, Science.

[3]  T. Liu,et al.  The chemical composition of pneumococcal C-polysaccharide. , 1963, The Journal of biological chemistry.

[4]  M. Cohn.  Natural History of the Myeloma , 1967 .

[5]  P. Plotz,et al.  Assignment of direct and facilitated hemolytic plaques in mice to specific immunoglobulin classes. , 1968, Journal of immunology.

[6]  M. Cohn,et al.  Reconstitution from subunits of the hapten binding sites and idiotypic determinants of mouse anti-phosphorylcholine myeloma proteins. , 1971, Journal of immunology.

[7]  M. Cohn,et al.  Characterization of the antibody to the C-carbohydrate produced by a transplantable mouse plasmacytoma. , 1969, Immunochemistry.

[8]  B. Prescott,et al.  Studies on the mechanism of the immunological paralysis induced in mice by pneumococcal polysaccharides. , 1955, Journal of immunology.

[9]  A. Nisonoff,et al.  QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES , 1970, The Journal of experimental medicine.

[10]  A. Tomasz.  Choline in the Cell Wall of a Bacterium: Novel Type of Polymer-Linked Choline in Pneumococcus , 1967, Science.

[11]  N. K. Jerne,et al.  Plaque Formation in Agar by Single Antibody-Producing Cells , 1963, Science.

[12]  G. Möller.  19S Antibody Production against Soluble Lipopolysaccharide Antigens by Individual Lymphoid Cells In Vitro , 1965, Nature.

[13]  J. Kapp,et al.  Anti-protein plaque-forming cells detected with high efficiency by the use of red cells coupled to bovine serum globulin through bis-diazo-benzidine. , 1970, Journal of immunology.

[14]  J. Pincus,et al.  Antibodies to Pneumococcal Polysaccharides: Relation between Binding and Electrophoretic Heterogeneity , 1968, Science.

[15]  M. Potter,et al.  Three IgA Myeloma Immunoglobulins from the BALB/c Mouse: Precipitation with Pneumococcal C Polysaccharide , 1968, Science.

[16]  L. Leserman,et al.  The third cell type required for the immune response of spleen cells in vitro. , 1971, Journal of immunology.