Nucleotide sequence and expression of a beta-tubulin gene from Plasmodium falciparum, a malarial parasite of man.

Genomic and cDNA clones, containing a Plasmodium falciparum beta-tubulin coding sequence (pf-bTub), were isolated and characterized. Comparison of the genomic sequence with the cDNA sequence showed that the malarial bTub-coding region is interrupted by two introns, the positions of which are not found in any beta-tubulin gene (btub) from other species. The gene appears to be present as a single-copy gene in the P. falciparum genome and is expressed as a 2.3-kb transcript both in the asexual blood stages and in the sexual stages (gametes/zygotes) of the parasite. The deduced polypeptide product of the pf-btub gene is a protein of 445 amino acids (aa) (Mr 49,517). Comparison of the aa sequence of pf-bTub with that of bTubs from other species revealed that the malarial protein shows a high degree of similarity to mammalian bTubs. Upon examination of the colchicine-binding sites of pf-bTub we predict that this tubulin probably has an altered sensitivity to this inhibitor.

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