ASSOCIATION OF PHYTOPLASMAS WITH A YELLOWS DISEASE OF CARROT IN ISRAEL

A carrot disease resembling carrot yellows has recently appeared in Israel. Electron microscopy and PCR analyses confirmed that the causative agent is a phytoplasma. This is the first report of carrot yellows in Israel. It was detected in five varieties from different regions of the country. Most of the infected plants carried the AY-type phytoplasma, but a few were found to carry a unique type of phytoplasma, hitherto not reported in carrots, which resembles X-disease agent. Both types of phytoplasma could be transmitted to periwinkle.

[1]  E. Seemüller,et al.  Current status of molecular classification of the phytoplasmas. , 1998 .

[2]  R. Bergès,et al.  Detection of phytoplasma infections in declining Populus nigra ‘Italica’ trees and molecular differentiation of the aster yellows phytoplasmas identified in various Populus species , 1997 .

[3]  J. Wyman,et al.  Variability in Aster Yellows Infectivity of Local Populations of the Aster Leafhopper (Homoptera: Cicadellidae) in Wisconsin , 1993 .

[4]  E. Seemüller,et al.  Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. , 1992 .

[5]  T. J. Morris,et al.  Cloning and Detection of DNA from a Nonculturable Plant Pathogenic Mycoplasma-like Organism , 1987, Science.

[6]  A. D. Rocha,et al.  Histochemical diagnosis of mycoplasma infections in Catharanthus roseus by means of a fluorescent DNA-binding agent, 4,6-diamidino-2-phenylindole-2HCl (DAPI) , 1986 .

[7]  L. N. Chiykowski,et al.  Herbaceous host plants of peach eastern X-disease agent , 1982 .

[8]  F. Zalom.  The Influence of Reflective Mulches and Lettuce Types on the Incidence of Aster Yellows and Abundance of its Vector, Macrosteles Fascifrons (Homoptera: Cicadelidae), in Minnesota , 1981, The Great Lakes Entomologist.

[9]  R. C. Sinha.  Purification and serology of mycoplasma-like organisms from aster yellows-infected plants , 1979 .

[10]  K. Maramorosch,et al.  ULTRASTRUCTURE OF THE ASTER YELLOWS AGENT: MYCOPLASMA‐LIKE BODIES IN SIEVE TUBE ELEMENTS OF NICOTIAN A RUSTICA * , 1973 .

[11]  Y. Doi,et al.  Mycoplasma- or PLT Group-like Microorganisms Found in the Phloem Elements of Plants Infected with Mulberry Dwarf, Potato Witches' Broom, Aster Yellows, or Paulownia Witches' Broom , 1967 .

[12]  B. E. Struckmeyer.  THE ANATOMIC RESPONSES OF DAUCUS CAROTA TO THE ASTER YELLOWS VIRUS , 1963 .

[13]  L. O. Kunkel.  STUDIES ON ASTER YELLOWS , 1926 .

[14]  R. Hammond,et al.  Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. , 1994 .

[15]  R. Davis,et al.  Cluster-specific polymerase chain reaction amplification of 16S rDNA sequences for detection and identification of mycoplasmalike organisms , 1993 .

[16]  D. Errampalli,et al.  Production of monospecific polyclonal antibodies against aster yellows mycoplasmalike organism-associated antigen , 1993 .

[17]  D. Errampalli,et al.  Incidence of yellows in carrot and lettuce and characterization of mycoplasmalike organism isolates in Oklahoma. , 1991 .

[18]  J. Tsai,et al.  Molecular cloning and detection of chromosomal and extrachromosomal DNA of mycoplasmalike organisms associated with witches'-broom disease of pigeon pea in Florida , 1991 .

[19]  A. Kushalappa,et al.  A survey of carrot diseases on muck soils in the southwestern part of Québec. , 1991 .

[20]  D. Orion,et al.  An electron microscopy study of cell wall lysis by Meloidogyne javanica gelatinous matrix , 1990 .

[21]  J. Tully,et al.  Epidemiological and phylogenetic studies on western X-disease mycoplasma-like organisms. , 1990 .

[22]  T. Chen,et al.  Comparison of monoclonal antibodies and polyclonal antibodies in detection of the aster yellows mycoplasmalike organism , 1986 .

[23]  A. D. Rocha,et al.  Detection of Mycoplasmalike Organisms In Situ by Indirect Immunofluorescence Microscopy , 1986 .

[24]  N. Benhamou,et al.  Detection of mycoplasmalike organism antigens from aster yellows-diseased plants by two serological procedures , 1983 .

[25]  J. Deeley.  Use of Dienes' Stain to Detect Plant Diseases Induced by Mycoplasmalike Organisms , 1979 .

[26]  M. Linn.  The yellows disease of lettuce and endive , 1940 .