Age, anatomic site, and the replication and differentiation of adipocyte precursors.

The effects of donor age and anatomic site on cellular replication and differentiation were studied in adipocyte precursors cloned from epididymal and perirenal depots of young, middle-aged, and senescent rats. As animals aged from 3 to 29 mo, there was a progressive reduction in the proportion of cells capable of extensive replication in both depots. An inverse relation between clonal capacity for replication and differentiation was found. This relation was affected by donor site but not age. Aging was, however, associated with a reduction in the frequency of clones capable of full differentiation into cells with single, large, central lipid inclusions. Hence, age and donor site may affect adipocyte precursor replication and differentiation by different mechanisms.

[1]  J. Kirkland,et al.  Varying capacities for replication of rat adipocyte precursor clones and adipose tissue growth. , 1989, The Journal of clinical investigation.

[2]  B. Franza,et al.  Fos and jun: The AP-1 connection , 1988, Cell.

[3]  Bruce M. Spiegelman,et al.  Nucleoprotein complexes that regulate gene expression in adipocyte differentiation: direct participation of c-fos , 1987, Cell.

[4]  B. Spiegelman,et al.  Nucleotide sequence and hormonal regulation of mouse glycerophosphate dehydrogenase mRNA during adipocyte and muscle cell differentiation. , 1987, The Journal of biological chemistry.

[5]  B. Spiegelman,et al.  Adipsin, the adipocyte serine protease: gene structure and control of expression by tumor necrosis factor. , 1986, Nucleic acids research.

[6]  H. Green,et al.  The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells. , 1986, The Journal of biological chemistry.

[7]  R. Scott,et al.  Regulation of the terminal event in cellular differentiation: biological mechanisms of the loss of proliferative potential , 1986, The Journal of cell biology.

[8]  J. R. Smith,et al.  Existence of high abundance antiproliferative mRNA's in senescent human diploid fibroblasts. , 1986, Science.

[9]  P. Djian,et al.  Adipocyte precursor clones vary in capacity for differentiation. , 1985, Metabolism: clinical and experimental.

[10]  H. Green,et al.  The activation of specific gene transcription in the adipose conversion of 3T3 cells , 1985, Journal of cellular physiology.

[11]  D. Roncari.  Pre-adipose cell replication and differentiation , 1984 .

[12]  P. Grimaldi,et al.  Adipose conversion of ob17 cells. Insulin acts solely as a modulator in the expression of the differentiation program. , 1984, Experimental cell research.

[13]  J. Remacle,et al.  Ageing of hamster embryo fibroblasts as the result of both differentiation and stochastic mechanisms , 1983, Experimental Gerontology.

[14]  B. Spiegelman,et al.  Fibronectin modulation of cell shape and lipogenic gene expression in 3t3-adipocytes , 1983, Cell.

[15]  P. Djian,et al.  Influence of anatomic site and age on the replication and differentiation of rat adipocyte precursors in culture. , 1983, The Journal of clinical investigation.

[16]  G. Martin,et al.  Comparative rates of decline in the primary cloning efficiencies of smooth muscle cells from the aging thoracic aorta of two murine species of contrasting maximum life span potentials. , 1983, The American journal of pathology.

[17]  L. Lajtha,et al.  STEM CELLS VERSUS STEM LINES , 1982, Annals of the New York Academy of Sciences.

[18]  E. Schultz,et al.  Skeletal muscle satellite cells: Changes in proliferation potential as a function of age , 1982, Mechanisms of Ageing and Development.

[19]  J. Rheinwald,et al.  Failure of hydrocortisone or growth factors to influence the senescence of fibroblasts in a new culture system for assessing replicative lifespan , 1981, Journal of cellular physiology.

[20]  E. Masoro,et al.  Changes in adipose mass and cellularity through the adult life of rats fed ad libitum or a life-prolonging restricted diet. , 1980, Journal of gerontology.

[21]  E. Masoro,et al.  Increasing adipocyte number as the basis for perirenal depot growth in adult rats. , 1978, Science.

[22]  J. R. Smith,et al.  Colony size distributions as a measure of in vivo and in vitro aging. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[23]  H. Green,et al.  Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes , 1977, Nature.

[24]  L. Hayflick.  THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS. , 1965, Experimental cell research.

[25]  J. Till,et al.  A STOCHASTIC MODEL OF STEM CELL PROLIFERATION, BASED ON THE GROWTH OF SPLEEN COLONY-FORMING CELLS. , 1964, Proceedings of the National Academy of Sciences of the United States of America.