Ultrafast FRET at fiber tips: Potential applications in sensitive remote sensing of molecular interaction

Abstract Forster resonance energy transfer (FRET) strategy is well adopted in fiber-optics for efficient sensor design. However, resonance type energy transfer from one molecule (donor) to other (acceptor) should meet few key properties including donor to acceptor energy migration in non-radiative way, which is hard to conclude from simply emission quenching of the donor, rather needs careful investigation of excited state lifetime of the donor molecule. In the present study, we have shown that the evanescent field of an optical fiber can be coupled to covalently attached donor (dansyl) molecules at the fiber tip. By using picosecond resolved time correlated single photon counting (TCSPC) we have demonstrated that dansyl at the fiber tip transfers energy to a well known DNA-intercalating dye ethidium upon surface adsorption of DNA at the fiber tip. Our ultrafast detection scheme selectively distinguishes the probe (dansyl) emission from the intrinsic emission of the fiber. The validation of the energy transfer mechanism to be of resonance type (FRET), allows us to estimate the distance between the probe dansyl and the surface adsorbed DNA. We have also used the setup for the remote sensing of the dielectric constant (polarity) of an environment as the excited state lifetime of the probe dansyl heavily depends on the polarity of the immediate host environment. FRET signal from a used fiber tip immediately after adsorption of DNA reveals stepwise surface desorption of the biomolecule in saline solution. The reusability of the fiber tip for sensing has also been demonstrated.

[1]  Subrata Batabyal,et al.  Ultrafast electron transfer in the recognition of different DNA sequences by a DNA-binding protein with different dynamical conformations , 2012, Journal of biomolecular structure & dynamics.

[2]  L. Stryer,et al.  Energy transfer: a spectroscopic ruler. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[3]  I.M.R. de Andrade Bruning.  Crude oil polarity measures quality, predicts behavior , 1991 .

[4]  J. Lakowicz.  Principles of fluorescence spectroscopy , 1983 .

[5]  H. Yeh,et al.  Single-quantum-dot-based DNA nanosensor , 2005, Nature materials.

[6]  A. Giri,et al.  Evanescent field: a potential light-tool for theranostics application. , 2014, The Review of scientific instruments.

[7]  Sheila A Grant,et al.  Effects of immobilization on a FRET immunosensor for the detection of myocardial infarction , 2005, Analytical and bioanalytical chemistry.

[8]  Ji Luo,et al.  A Silver Nanoparticle-Modified Evanescent Field Optical Fiber Sensor for Methylene Blue Detection , 2013, Sensors.

[9]  Subrata Batabyal,et al.  Ultrafast dynamics of solvation and charge transfer in a DNA-based biomaterial. , 2014, Chemistry, an Asian journal.

[10]  Laura Marcu,et al.  Percutaneous fiber-optic sensor for chronic glucose monitoring in vivo. , 2008, Biosensors & bioelectronics.

[11]  Th. Förster.  Zwischenmolekulare Energiewanderung und Fluoreszenz , 1948 .

[12]  S.A. Grant,et al.  Development of a FRET based fiber-optic biosensor for early detection of myocardial infarction , 2004, The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[13]  U. Schubert,et al.  Poly(2-oxazoline) functionalized surfaces: from modification to application. , 2013, Chemical Society reviews.

[14]  A. Zewail,et al.  Femtosecond studies of protein-DNA binding and dynamics: histone I. , 2001, Chemphyschem : a European journal of chemical physics and physical chemistry.

[15]  Gösta. Åkerlöf,et al.  The Dielectric Constant of Dioxane—Water Mixtures between 0 and 80° , 1936 .

[16]  David L. Meadows,et al.  Design, manufacture and characterization of an optical fiber glucose affinity sensor based on an homogeneous fluorescence energy transfer assay system , 1993 .

[17]  Jeff Hecht,et al.  City of Light: The Story of Fiber Optics , 1999 .

[18]  S. Grant,et al.  Development of a novel FRET immunosensor technique. , 2003, Biosensors & bioelectronics.

[19]  J. Schultz,et al.  A fluorescence affinity hollow fiber sensor for continuous transdermal glucose monitoring. , 2000, Analytical chemistry.

[20]  Sungho Ko,et al.  A novel FRET-based optical fiber biosensor for rapid detection of Salmonella typhimurium. , 2006, Biosensors & bioelectronics.

[21]  T. Watson,et al.  Fluorescence lifetime endoscopy using TCSPC for the measurement of FRET in live cells , 2010, Optics express.

[22]  Pekka Hänninen,et al.  Protein quantification using resonance energy transfer between donor nanoparticles and acceptor quantum dots. , 2013, Analytical chemistry.

[23]  Mustafa Ozmen,et al.  Surface Modification of Glass Beads with an Aminosilane Monolayer , 2006 .

[24]  S. Pal,et al.  Interaction of Hoechst 33258 and ethidium with histone1-DNA condensates. , 2007, Biomacromolecules.

[25]  P. Shankar,et al.  A review of fiber-optic biosensors , 2007 .

[26]  S. Pal,et al.  Ultrafast dynamics in a nanocage of enzymes: solvation and fluorescence resonance energy transfer in reverse micelles. , 2005, Journal of colloid and interface science.

[27]  S. Pal,et al.  Simultaneous binding of minor groove binder and intercalator to dodecamer DNA: importance of relative orientation of donor and acceptor in FRET. , 2007, The journal of physical chemistry. B.