Ultrasonic particle concentration in a line-driven cylindrical tube.

Acoustic particle manipulation has many potential uses in flow cytometry and microfluidic array applications. Currently, most ultrasonic particle positioning devices utilize a quasi-one-dimensional geometry to set up the positioning field. A transducer fit with a quarter-wave matching layer, locally drives a cavity of width one-half wavelength. Particles within the cavity experience a time-averaged drift force that transports them to a nodal position. Present research investigates an acoustic particle-positioning device where the acoustic excitation is generated by the entire structure, as opposed to a localized transducer. The lowest-order structural modes of a long cylindrical glass tube driven by a piezoceramic with a line contact are tuned, via material properties and aspect ratio, to match resonant modes of the fluid-filled cavity. The cylindrical geometry eliminates the need for accurate alignment of a transducer/reflector system, in contrast to the case of planar or confocal fields. Experiments show that the lower energy density in the cavity, brought about through excitation of the whole cylindrical tube, results in reduced cavitation, convection, and thermal gradients. The effects of excitation and material parameters on concentration quality are theoretically evaluated, using two-dimensional elastodynamic equations describing the fluid-filled cylindrical shell with a line excitation.

[1]  L. Pretorius,et al.  Separation of solid-liquid suspensions with ultrasonic acoustic energy , 1997 .

[2]  J Dual,et al.  Positioning of small particles by an ultrasound field excited by surface waves. , 2004, Ultrasonics.

[3]  T. Okada,et al.  Particle characterization and separation by a coupled acoustic-gravity field. , 2001, Analytical chemistry.

[4]  Particle motion in resonance tubes , 1998 .

[5]  K Yasuda,et al.  Using acoustic radiation force as a concentration method for erythrocytes. , 1997, The Journal of the Acoustical Society of America.

[6]  Steven W Graves,et al.  Nozzle design parameters and their effects on rapid sample delivery in flow cytometry. , 2002, Cytometry.

[7]  W. T. Coakley,et al.  Particle column formation in a stationary ultrasonic field , 1992 .

[8]  Kazuhiko Yamanouchi,et al.  Ultrasonic Micromanipulation of Small Particles in Liquid , 1994 .

[9]  D. Feke,et al.  Separation of dispersed phases from liquids in acoustically driven chambers , 1993 .

[10]  Takahi Hasegawa,et al.  Acoustic‐Radiation Force on a Solid Elastic Sphere , 1969 .

[11]  David J. Clarke,et al.  Cell manipulation in ultrasonic standing wave fields , 2007 .

[12]  J Dual,et al.  Micro-manipulation of small particles by node position control of an ultrasonic standing wave. , 2002, Ultrasonics.

[13]  H. Schmidt,et al.  A numerically stable global matrix method for cylindrically layered shells excited by ring forces , 1994 .

[14]  W D O'Brien,et al.  Quantitative assessment of the germicidal efficacy of ultrasonic energy , 1991, Applied and environmental microbiology.

[15]  A. Kundt,et al.  Ueber longitudinale Schwingungen und Klangfiguren in cylindrischen Flüssigkeitssäulen , 1874 .

[16]  Ica Manas-Zloczower,et al.  Fractionation of mixed particulate solids according to compressibility using ultrasonic standing wave fields , 1995 .

[17]  Alexander A. Doinikov,et al.  Acoustic radiation interparticle forces in a compressible fluid , 2001, Journal of Fluid Mechanics.

[18]  L. Crum,et al.  Acoustic Cavitation , 1982 .

[19]  T J Mason,et al.  The development and evaluation of ultrasound for the treatment of bacterial suspensions. A study of frequency, power and sonication time on cultured Bacillus species. , 2003, Ultrasonics sonochemistry.

[20]  Shin-ichiro Umemura,et al.  Concentration and Fractionation of Small Particles in Liquid by Ultrasound , 1995 .

[21]  Takahi Hasegawa,et al.  Acoustic radiation force on fused silica spheres, and intensity determination , 1975 .

[22]  Robert E. Apfel,et al.  Acoustic cavitation prediction , 1978 .

[23]  K. Shuster,et al.  The effect of centreline particle concentration in a wave tube , 1996 .

[24]  Yoshiki Yamakoshi,et al.  Micro particle trapping by opposite phases ultrasonic travelling waves , 1998 .

[25]  Yoshizo Matsuno,et al.  Migration of suspended particles in plane stationary ultrasonic field , 1981 .

[26]  D. Barrow,et al.  Microparticle manipulation in millimetre scale ultrasonic standing wave chambers. , 1998, Ultrasonics.

[27]  P. Riseborough The small polaron with nonlinear electron-phonon interactions , 1984 .

[28]  Jeremy J. Hawkes,et al.  A laminar flow expansion chamber facilitating downstream manipulation of particles concentrated using an ultrasonic standing wave , 1998 .

[29]  R. Allman,et al.  Ultrasonic manipulation of particles and cells. Ultrasonic separation of cells. , 1994, Bioseparation.

[30]  K. Yosioka,et al.  Acoustic radiation pressure on a compressible sphere , 1955 .

[31]  Louis Vessot King,et al.  On the Acoustic Radiation Pressure on Spheres , 1934 .

[32]  E. Carr Everbach,et al.  Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubbles , 1998, Applied and Environmental Microbiology.

[33]  W. Coakley,et al.  Transport and harvesting of suspended particles using modulated ultrasound. , 1991, Ultrasonics.

[34]  J. Hawkes,et al.  Analytical scale ultrasonic standing wave manipulation of cells and microparticles. , 2000, Ultrasonics.

[35]  Hans M. Hertz,et al.  Standing-wave Acoustic Trap For Nonintrusive Positioning of Microparticles , 1995 .