Changes in bioactive compounds and oxidative enzymes of fresh-cut pomegranate arils during storage as affected by deficit irrigation and postharvest vapor heat treatments

The effect of postharvest vapor heat treatments at 95℃ (4, 7, and 10 s) regarding a conventional sanitizing treatment with 100 mg NaClO l−1 on enzyme activities (phenylalanine ammonia lyase, polyphenol oxidase, and peroxidase), phenolic content, and total antioxidant capacity of fresh-cut pomegranates arils throughout 18 days at 5℃ was studied. Furthermore, the effect of two sustained deficit irrigation (SDI) strategies, compared to a standardly irrigated control (CTRL), was also studied on such quality parameters throughout storage. Arils from CTRL-irrigated fruit registered phenylalanine ammonia lyase, peroxidase, and polyphenol oxidase initial activities of 60.6, 382, and 14.4 U g-1 fw, respectively. Arils from sustained deficit irrigation fruit registered 46–58% lower phenylalanine ammonia lyase values while polyphenol oxidase and peroxidase activities did not register great variants (<9%) among both sustained deficit irrigation treatments. Postharvest vapor heat treatments enhanced phenylalanine ammonia lyase activity in those samples from sustained deficit irrigation fruit although no great peroxidase and polyphenol oxidase (<2–5%) increases were observed. Arils from SDI1 fruit registered higher phenolic content than those values reported for CTRL samples. However, phenolic compounds decreased during storage, in a greater extent for sustained deficit irrigation samples, although 7 s arils achieved better phenolic compounds retention in sustained deficit irrigation samples. Vapor heat treatments reduced up to twofold the total antioxidant capacity losses observed in samples sanitized by conventional NaOCl treatment during shelf life. Conclusively, postharvest vapor heat treatment for 7 and 10 s used to extend the shelf life of pomegranate arils up to 18 days at 5℃ reduced the losses of health-promoting compounds during storage compared to conventional NaOCl sanitizing treatment.

[1]  P. Gómez,et al.  Deficit irrigation strategies combined with controlled atmosphere preserve quality in early peaches , 2015, Food science and technology international = Ciencia y tecnologia de los alimentos internacional.

[2]  P. Gómez,et al.  Quality changes of fresh-cut pomegranate arils during shelf life as affected by deficit irrigation and postharvest vapour treatments. , 2015, Journal of the science of food and agriculture.

[3]  A. Guadarrama,et al.  Specific phenolic compounds and sensory properties of a new dealcoholized red wine with pomegranate (Punica granatum L.) extract , 2014, Food science and technology international = Ciencia y tecnologia de los alimentos internacional.

[4]  Z. Zamani,et al.  Combined effect of heat treatment, UV-C and superatmospheric oxygen packing on phenolics and browning related enzymes of fresh-cut pomegranate arils , 2013 .

[5]  M. Peña,et al.  Effect of sustained deficit irrigation on physicochemical properties, bioactive compounds and postharvest life of pomegranate fruit (cv. ‘Mollar de Elche’) , 2013 .

[6]  C. Rojas-Argudo,et al.  Effect of sustained and regulated deficit irrigation on fruit quality of pomegranate cv. ‘Mollar de Elche’ at harvest and during cold storage , 2013 .

[7]  Younes Mostofi,et al.  Hot water, UV-C and superatmospheric oxygen packaging as hurdle techniques for maintaining overall quality of fresh-cut pomegranate arils. , 2013, Journal of the science of food and agriculture.

[8]  Haile Ma,et al.  Quantitative determination of major polyphenol constituents in pomegranate products. , 2012, Food chemistry.

[9]  P. Gómez,et al.  Moderate UV-C pretreatment as a quality enhancement tool in fresh-cut Bimi® broccoli , 2011 .

[10]  Reinhold Carle,et al.  Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD-ESI/MS(n). , 2011, Food chemistry.

[11]  C. Watkins,et al.  Involvement of ethylene in browning development of controlled atmosphere-stored ‘Empire’ apple fruit , 2011 .

[12]  D. Valero,et al.  Vapour treatments with methyl salicylate or methyl jasmonate alleviated chilling injury and enhanced antioxidant potential during postharvest storage of pomegranates , 2011 .

[13]  D. Camejo,et al.  Antioxidant system and protein pattern in peach fruits at two maturation stages. , 2010, Journal of agricultural and food chemistry.

[14]  J. R. Porter,et al.  Anthocyanins and polyphenol oxidase from dried arils of pomegranate (Punica granatum L.) , 2010 .

[15]  Lucrezia Sergio,et al.  Termostabilnost topljivih i vezanih peroksidaza iz artičoke te matematički model njihove kinetike inaktivacije , 2007 .

[16]  R. Serraj,et al.  Growth, water relations and antioxidant defence mechanisms of olive (Olea europaea L.) subjected to Partial Root Drying (PRD) and Regulated Deficit Irrigation (RDI) , 2007 .

[17]  F. Tomás-Barberán,et al.  Enriched ozone atmosphere enhances bioactive phenolics in seedless table grapes after prolonged shelf life , 2007 .

[18]  A. Cardinali,et al.  Thermostability of Soluble and Bound Peroxidases from Artichoke and a Mathematical Model of Its Inactivation Kinetics , 2007 .

[19]  F. García-Carmona,et al.  Latent polyphenol oxidase from quince fruit pulp (Cydonia oblonga): purification, activation and some properties , 2006 .

[20]  P. Rapisarda,et al.  Effect of thermal treatments on antioxidant and antiradical activity of blood orange juice , 2004 .

[21]  L. Cisneros-Zevallos.  The Use of Controlled Postharvest Abiotic Stresses as a Tool for Enhancing the Nutraceutical Content and Adding-Value of Fresh Fruits and Vegetables , 2003 .

[22]  G. Qin,et al.  Enhancement of biocontrol efficacy of antagonistic yeasts by salicylic acid in sweet cherry fruit , 2003 .

[23]  V. Gökmen,et al.  Organic Acids and Phenolic Compounds in Pomegranates (Punica granatum L.) Grown in Turkey , 2002 .

[24]  C. Rice-Evans,et al.  Antioxidant activity applying an improved ABTS radical cation decolorization assay. , 1999, Free radical biology & medicine.

[25]  J. Strain,et al.  Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. , 1999, Methods in enzymology.

[26]  M. Cano,et al.  Characterisation of 'starking' apple polyphenoloxidase , 1998 .

[27]  N. Nishiyama,et al.  Phenolic Contents and L-Phenylalanine Ammonia-Lyase Activity in Peach Fruit , 1996 .

[28]  C. Berset,et al.  Use of a Free Radical Method to Evaluate Antioxidant Activity , 1995 .

[29]  J. C. Suatoni,et al.  Determination of Phenolic Compounds by HPLC , 1982 .

[30]  L. Vámos-Vigyázó,et al.  Polyphenol oxidases and peroxidases in fruits and vegetables , 1981 .

[31]  L. Vámos-Vigyázó,et al.  Polyphenol oxidase and peroxidase in fruits and vegetables. , 1981, Critical reviews in food science and nutrition.

[32]  H. V. Marsh,et al.  Effects of water stress on the activities of three enzymes in maize seedlings. , 1971, Plant physiology.