Leifsonia pindariensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas, and emended description of the genus Leifsonia.

Strain PON10(T) is a yellow-pigmented, Gram-positive, aerobic, motile, rod-shaped bacterium isolated from the Pindari glacier of the Indian Himalayas. The cell-wall peptidoglycan contained DL-diaminobutyric acid as the diamino acid. The predominant fatty acids were anteiso-C(15:0), anteiso-C(17:0) and iso-C(16:0) and the major isoprenoid quinones were MK-10 and MK-11. Based on the above characteristics, strain PON10(T) was assigned to the genus Leifsonia. blast sequence similarity results indicated that Leifsonia ginsengi and Leifsonia poae were the nearest relatives, with 16S rRNA gene sequence similarity of 97.0 and 96.8% to the respective type strains. A difference of 3% in the 16S rRNA gene sequence indicated that PON10(T) represents a novel species of the genus Leifsonia, and therefore DNA-DNA hybridization was not done. In addition, PON10(T) showed a number of differences from Leifsonia ginsengi and Leifsonia poae with respect to phenotypic and chemotaxonomic characteristics. Thus, based on the differences it exhibited from Leifsonia ginsengi and Leifsonia poae, strain PON10(T) was identified as representing a novel species named Leifsonia pindariensis sp. nov. The type strain is PON10(T) (=LMG 24222(T) =MTCC9128(T)). An emended description of the genus Leifsonia is also presented.

[1]  Ying Huang,et al.  Leifsonia ginsengi sp. nov., isolated from ginseng root. , 2007, International journal of systematic and evolutionary microbiology.

[2]  T. Satyanarayana,et al.  Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil. , 2007, International journal of systematic and evolutionary microbiology.

[3]  S. Shivaji,et al.  Exiguobacterium indicum sp. nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan mountain ranges of India. , 2006, International journal of systematic and evolutionary microbiology.

[4]  R. Kroppenstedt,et al.  Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas. , 2006, International journal of systematic and evolutionary microbiology.

[5]  R. Kroppenstedt,et al.  Agrococcus lahaulensis sp. nov., isolated from a cold desert of the Indian Himalayas. , 2006, International journal of systematic and evolutionary microbiology.

[6]  S. Mayilraj,et al.  Ornithinimicrobium kibberense sp. nov., isolated from the Indian Himalayas. , 2006, International journal of systematic and evolutionary microbiology.

[7]  R. Kroppenstedt,et al.  Dietzia kunjamensis sp. nov., isolated from the Indian Himalayas. , 2006, International journal of systematic and evolutionary microbiology.

[8]  S. Mayilraj,et al.  Rhodococcus kroppenstedtii sp. nov., a novel actinobacterium isolated from a cold desert of the Himalayas, India. , 2006, International journal of systematic and evolutionary microbiology.

[9]  F. Garcia-Pichel,et al.  Belnapia moabensis gen. nov., sp. nov., an alphaproteobacterium from biological soil crusts in the Colorado Plateau, USA. , 2006, International journal of systematic and evolutionary microbiology.

[10]  S. Mayilraj,et al.  Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas. , 2005, International journal of systematic and evolutionary microbiology.

[11]  S. Shivaji,et al.  Dyadobacter hamtensis sp. nov., from Hamta glacier, located in the Himalayas, India. , 2005, International journal of systematic and evolutionary microbiology.

[12]  S. Shivaji,et al.  Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. , 2005, International journal of systematic and evolutionary microbiology.

[13]  M. Kimura.  A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences , 1980, Journal of Molecular Evolution.

[14]  T. Chakrabarti,et al.  Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. , 2005, International journal of systematic and evolutionary microbiology.

[15]  Sudhir Kumar,et al.  MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment , 2004, Briefings Bioinform..

[16]  E. Stackebrandt,et al.  Psychrophilic pseudomonads from Antarctica: Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov. , 2004, International journal of systematic and evolutionary microbiology.

[17]  S. Shivaji,et al.  Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. , 2004, International journal of systematic and evolutionary microbiology.

[18]  J. Prakash,et al.  Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov., psychrophiles from a pond in Antarctica. , 2003, International journal of systematic and evolutionary microbiology.

[19]  J. Tiedje,et al.  Agreia bicolorata gen. nov., sp. nov., to accommodate actinobacteria isolated from narrow reed grass infected by the nematode Heteroanguina graminophila. , 2001, International journal of systematic and evolutionary microbiology.

[20]  F. Rainey,et al.  Subtercola boreus gen. nov., sp. nov. and Subtercola frigoramans sp. nov., two new psychrophilic actinobacteria isolated from boreal groundwater. , 2000, International journal of systematic and evolutionary microbiology.

[21]  S. Shivaji,et al.  Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. , 2000, International journal of systematic and evolutionary microbiology.

[22]  K. Komagata,et al.  Leifsonia gen. nov., a genus for 2,4-diaminobutyric acid-containing actinomycetes to accommodate "Corynebacterium aquaticum" Leifson 1962 and Clavibacter xyli subsp. cynodontis Davis et al. 1984. , 1999, The Journal of general and applied microbiology.

[23]  M. Meier,et al.  Mass balance of mountain and subpolar glaciers: a new global assessment , 1997 .

[24]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[25]  Y. Ageta,et al.  Predictions of changes of glacier mass balance in the Nepal Himalaya and Tibetan Plateau: a case study of air temperature increase for three glaciers , 1992, Annals of Glaciology.

[26]  B. Lânyi.  1 Classical and Rapid Identification Methods for Medically Important Bacteria , 1988 .

[27]  K. Komagata,et al.  4 Lipid and Cell-Wall Analysis in Bacterial Systematics , 1988 .

[28]  J. Tamaoka.  [26] Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography , 1986 .

[29]  J. Tamaoka.  Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. , 1986, Methods in enzymology.

[30]  Michael J. Davis,et al.  Clavibacter: a New Genus Containing Some Phytopathogenic Coryneform Bacteria, Including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp. cynodontis subsp. nov., Pathogens That Cause Ratoon Stunting Disease of Sugarcane and Bermudagrass Stunting Disease† , 1984 .

[31]  J. Tamaoka,et al.  Analysis of bacterial menaquinone mixtures by high performance liquid chromatography , 1983 .

[32]  M. Collins,et al.  Distribution of menaquinones in actinomycetes and corynebacteria. , 1977, Journal of general microbiology.

[33]  E. Leifson.  The Bacterial Flora of Distilled and Stored Water. III. new species of the genera Corynebacterium, Flavobacterium, Spirillum and Pseudomonas , 1962 .