Membrane systems of guinea pig myocardium: Ultrastructure and morphometric studies

The structure and quantitative contribution of membrane systems (transverse‐axal tubular system [TATS] and sarcoplasmic reticulum [SR]) have been investigated in the heart of the adult guinea pig. Although previous quantitative studies have been made of guinea pig myocardium, this is the first such study that has utilized tissue in which membrane system elements were clearly identified by selective staining (in this case by the osmium‐ferrocyanide [OsFeCN] postfixation method). Both membrane systems are highly developed in ventricular cells, but a TATS is essentially absent from atrial myocytes. The ventricular TATS consists principally of large‐bore elements which may be oriented transversely, axially, or obliquely, making numerous anastomoses with one another to form a highly interconnected system of extracellular spaces that penetrate to all myoplasmic depths of the ventricular cell. The cell coat that lines the lumina of these tubules is structured, containing fibrillar structures that run along the length of the tubule. The volume fraction (Vv) of the ventricular TATS is low (2.5–3.2%), in consideration of the qualitative prominence of the TATS in these cells.

[1]  V. Ferrans,et al.  Morphological study of sarcoplasmic reticulum in the atrioventricular node and bundle cells in guinea pig hearts. , 1987, The American journal of anatomy.

[2]  A. Gerdes,et al.  Comparison of regional differences in cardiac myocyte dimensions in rats, hamsters, and guinea pigs , 1987, The Anatomical record.

[3]  M. Reedy,et al.  Developmental Changes in the Ultrastructure and Sarcomere Shortening of the Isolated Rabbit Ventricular Myocyte , 1987, Circulation research.

[4]  L. Peachey,et al.  Energy filtering electron microscopy (EFEM) of thick sections of embedded biological tissues at 80 kV , 1987 .

[5]  S. Fleischer,et al.  Characterization of the junctional face membrane from terminal cisternae of sarcoplasmic reticulum , 1986, The Journal of cell biology.

[6]  N. Sperelakis,et al.  The sarcoplasmic reticulum of mouse heart: its divisions, configurations, and distribution. , 1985, Journal of ultrastructure research.

[7]  K. Campbell,et al.  Ultrastructural localization of calsequestrin in adult rat atrial and ventricular muscle cells , 1985, The Journal of cell biology.

[8]  N. Sperelakis,et al.  The transverse-axial tubular system (TATS) of mouse myocardium: its morphology in the developing and adult animal. , 1984, The American journal of anatomy.

[9]  P. Dolber,et al.  Corbular sarcoplasmic reticulum of rabbit cardiac muscle. , 1984, Journal of ultrastructure research.

[10]  L. Pott,et al.  Atrial muscle cells from hearts of adult guinea-pigs in culture: a new preparation for cardiac cellular electrophysiology. , 1983, European journal of cell biology.

[11]  D. Scales.  Aspects of the mammalian cardiac sarcotubular system revealed by freeze fracture electron microscopy. , 1981, Journal of molecular and cellular cardiology.

[12]  S. Sartore,et al.  Myosin types and fiber types in cardiac muscle. I. Ventricular myocardium , 1981, The Journal of cell biology.

[13]  N. Sperelakis,et al.  Membrane systems in skeletal muscle of the lizard Anolis carolinensis. , 1980, Journal of ultrastructure research.

[14]  J. Sommer,et al.  Freeze-Fracture Appearance of Rabbit Cardiac Sarcoplasmic Reticulum , 1980 .

[15]  K. Rybicka.  Sarcoplasmic reticulum in the conducting fibers of the dog heart , 1977, The Anatomical record.

[16]  N. Sperelakis,et al.  Cytochemical staining procedures selective for sarcotubular systems of muscle: modifications and applications. , 1977, Journal of ultrastructure research.

[17]  E. Johnson,et al.  Cardiac muscle: an attempt to relate structure to function. , 1976, Journal of molecular and cellular cardiology.

[18]  A. A. Walker,et al.  Comparison of Contractile Performance of Canine Atrial and Ventricular Muscles , 1975, Circulation research.

[19]  J Shay,et al.  Economy of effort in electron microscope morphometry. , 1975, The American journal of pathology.

[20]  C. Devine,et al.  Freeze fracture studies of membrane systems in vertebrate muscle. I. Striated muscle. , 1975, Journal of ultrastructure research.

[21]  N. Sperelakis,et al.  SPHEROIDAL BODIES IN THE JUNCTIONAL SARCOPLASMIC RETICULUM OF LIZARD MYOCARDIAL CELLS , 1974, The Journal of cell biology.

[22]  N. Sperelakis,et al.  An orderly lattice of axial tubules which interconnect adjacent transverse tubules in guinea-pig ventricular myocardium. , 1971, Journal of molecular and cellular cardiology.

[23]  P. Jewett,et al.  CARDIAC MUSCLE , 1971, The Journal of Cell Biology.

[24]  A. Martínez-Palomo,et al.  TRANSITIONAL CARDIAC CELLS OF THE CONDUCTIVE SYSTEM OF THE DOG HEART , 1970, The Journal of cell biology.

[25]  D. Rayns,et al.  Surface features of striated muscle. I. Guinea-pig cardiac muscle. , 1968, Journal of cell science.

[26]  D. Rayns,et al.  Transverse Tubule Apertures in Mammalian Myocardial Cells: Surface Array , 1967, Science.

[27]  F. O. Simpson.  THE TRANSVERSE TUBULAR SYSTEM IN MAMMALIAN MYOCARDIAL CELLS. , 1965, The American journal of anatomy.

[28]  R. Coggeshall,et al.  A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY , 1965, The Journal of cell biology.

[29]  L. Barr,et al.  A STUDY OF THE STRUCTURE AND DISTRIBUTION OF THE NEXUS , 1964, The Journal of cell biology.

[30]  D. Spiro,et al.  THE ULTRASTRUCTURE OF MAMMALIAN CARDIAC MUSCLE , 1961, The Journal of biophysical and biochemical cytology.

[31]  F. Sjöstrand,et al.  The ultrastructure of the intercalated discs of frog, mouse and guinea pig cardiac muscle. , 1958, Journal of ultrastructure research.

[32]  N. Severs,et al.  Rough endoplasmic reticulum in the adult mammalian cardiac muscle cell. , 1985, Journal of submicroscopic cytology.

[33]  N. Sperelakis,et al.  Structures located at the levels of the Z bands in mouse ventricular myocardial cells. , 1980, Tissue & cell.

[34]  T. Gotoh,et al.  Quantitative studies on the ultrastructural differentiation and growth of mammalian cardiac muscle cells. I. The atria and ventricles of the rat. , 1980, Acta anatomica.

[35]  T. Gotoh,et al.  Quantitative studies on the ultrastructural differentiation and growth of mammalian cardiac muscle cells. II. The atria and ventricles of the guinea pig. , 1980, Acta anatomica.

[36]  R. Waugh,et al.  Comparative stereology of the mouse and finch left ventricle. , 1978, Tissue & cell.

[37]  J. Tranum-Jensen.  The Fine Structure of the Sinus Node: a Survey , 1978 .

[38]  A. Seligman,et al.  A STUDY ON FERROCYANIDE-REDUCED OSMIUM TETROXIDE AS A STAIN AND CYTOCHEMICAL AGENT , 1976 .

[39]  J. Ledingham,et al.  THE ULTRASTRUCTURE OF VENTRICULAR AND ATRIAL MYOCARDIUM , 1973 .