Suitability of total coliform β-D-galactosidase activity and CFU counts in monitoring faecal contamination of environmental water samples

Total coliforms are a group of bacteria found in high numbers in mammalian intestines; hence their presence in water indicates the possible contamination with faecal material. Total and faecal coliform counts were monitored over a period of 18 months using mFC, m-Endo and CM1046 media together with enzymatic assays on 215 environmental water samples obtained from the Eastern Cape Province of South Africa. A positive correlation, with an R2 value of 0.9393 was observed between faecal and total coliform colony units employing mFc and m-Endo media, and 0.8818 using CM1046 media. Also, a positive correlation was observed between Escherichia coli colony-forming units and β-d-galactosidase (B-GAL) activity (R2=0.8542). Overall, this study indicated that faecal contamination of environmental water samples could be monitored by measuring total coliform β-galactosidase activity and total coliform colony-forming units.

[1]  Nicholas J. Ashbolt,et al.  Indicators of microbial water quality , 2001 .

[2]  M. Hänninen,et al.  Campylobacter spp., Giardia spp., Cryptosporidium spp., Noroviruses, and Indicator Organisms in Surface Water in Southwestern Finland, 2000-2001 , 2004, Applied and Environmental Microbiology.

[3]  A. Camper,et al.  Enumeration of indicator bacteria exposed to chlorine. , 1983, Advances in applied microbiology.

[4]  B. Pletschke,et al.  Comparison of the direct enzyme assay method with the membrane filtration technique in the quantification and monitoring of microbial indicator organisms - seasonal variations in the activities of coliforms and E.coli, temperature and pH , 2009 .

[5]  Stephen A. Martin,et al.  Accurate Mass Measurements Using MALDI-TOF with Delayed Extraction , 1997, Journal of protein chemistry.

[6]  A. Heim,et al.  Prevalence, quantification and typing of adenoviruses detected in river and treated drinking water in South Africa , 2005, Journal of applied microbiology.

[7]  Arie H. Havelaar,et al.  Guidelines: the current position , 2001 .

[8]  Ashok Kumar,et al.  Removal of fluoride, arsenic and coliform bacteria by modified homemade filter media from drinking water. , 2008, Bioresource technology.

[9]  B. Erasmus,et al.  Assessment of cell culture and polymerase chain reaction procedures for the detection of polioviruses in wastewater. , 1999, Bulletin of the World Health Organization.

[10]  I. Miettinen,et al.  Comparison of media for enumeration of coliform bacteria and Escherichia coli in non-disinfected water. , 2007, Journal of microbiological methods.

[11]  A. Farnleitner,et al.  Hydrolysis of 4-methylumbelliferyl-beta-D-glucuronide in differing sample fractions of river waters and its implication for the detection of fecal pollution. , 2002, Water research.

[12]  H. Schraft,et al.  Enumeration of heterotrophs, fecal coliforms and Escherichia coli in water: comparison of 3M Petrifilm plates with standard plating procedures. , 2005, Journal of microbiological methods.

[13]  B. Pletschke,et al.  Properties of in situ Escherichia coli -D-glucuronidase (GUS): evaluation of chemical interference on the direct enzyme assay for faecal pollution detection in water , 2006 .

[14]  Pierre Servais,et al.  Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. , 2002, Journal of microbiological methods.

[15]  LeVerne D. Hudson,et al.  Coliforms in a water distribution system: a remedial approach , 1983 .

[16]  S. Weisberg,et al.  Comparison of total coliform, fecal coliform, and enterococcus bacterial indicator response for ocean recreational water quality testing. , 2003, Water research.

[17]  D. Deere,et al.  Water Quality : Guidelines , Standards and Health , 2003 .

[18]  S. Laufer,et al.  MALDI-TOF MS analysis of urinary nucleosides , 2005, Journal of the American Society for Mass Spectrometry.

[19]  D A Mossel,et al.  Taxonomical changes in intestinal (faecal) enterococci and streptococci: consequences on their use as indicators of faecal contamination in drinking water. , 1996, The Journal of applied bacteriology.

[20]  F. Kansiime,et al.  Discrimination Efficacy of Fecal Pollution Detection in Different Aquatic Habitats of a High-Altitude Tropical Country, Using Presumptive Coliforms, Escherichia coli, and Clostridium perfringens Spores , 2005, Applied and Environmental Microbiology.

[21]  P. Servais,et al.  β‐d‐glucuronidase activity assay to assess viable Escherichia coli abundance in freshwaters , 2005, Letters in applied microbiology.

[22]  M. Lechevallier,et al.  Injured coliforms in drinking water , 1986, Applied and environmental microbiology.

[23]  J. Whitelegge.  Thylakoid membrane proteomics , 2004, Photosynthesis Research.

[24]  S. Edberg,et al.  Advances in the bacteriology of the coliform group: their suitability as markers of microbial water safety. , 2001, Annual review of microbiology.

[25]  Joan B. Rose,et al.  Reevaluation of Microbial Water Quality: Powerful New Tools for Detection and Risk Assessment , 2001 .

[26]  C. Fricker,et al.  A comparison of the International Standards Organisation reference method for the detection of coliforms and Escherichia coli in water with a defined substrate procedure , 2003, Journal of applied microbiology.

[27]  B. Barbeau,et al.  Evaluation of Bacillus subtilis and coliphage MS2 as indicators of advanced water treatment efficiency. , 2003, Water science and technology : a journal of the International Association on Water Pollution Research.

[28]  M. Hänninen,et al.  Evaluation of the lactose Tergitol-7, m-Endo LES, Colilert 18, Readycult Coliforms 100, Water-Check-100, 3M Petrifilm EC and DryCult Coliform test methods for detection of total coliforms and Escherichia coli in water samples. , 2006, Water research.