Ras-regulated signaling processes in Saccharomyces cerevisiae.

Molecular genetics has proved quite successful in identifying the components of RAS-mediated signal transduction in the yeast Saccharomyces cerevisiae and in defining the nature of their interactions. Recently, the emphasis has shifted to a biochemical approach as the processes of guanine nucleotide exchange, GTPase stimulation activity and posttranslational modification of Ras proteins have all been reproduced in vitro.

[1]  K. Matsumoto,et al.  IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein. , 1990, Molecular and cellular biology.

[2]  Susan S. Brown,et al.  Evidence for a functional link between profilin and CAP in the yeast S. cerevisiae , 1991, Cell.

[3]  F. Tamanoi,et al.  Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[4]  F. Tamanoi,et al.  Mutants of Saccharomyces cerevisiae defective in the farnesylation of Ras proteins. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Hofsteenge,et al.  alpha- and beta-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure. , 1990, Biochemistry.

[6]  M. Wigler,et al.  CAP is a bifunctional component of the Saccharomyces cerevisiae adenylyl cyclase complex , 1991, Molecular and cellular biology.

[7]  J. Broach,et al.  The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras , 1991, Molecular and cellular biology.

[8]  M. Wigler,et al.  Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein , 1990, Cell.

[9]  H. Horvitz,et al.  Caenorhabditis elegans ras gene let-60 acts as a switch in the pathway of vulval induction , 1990, Nature.

[10]  M. Jacquet,et al.  SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae , 1991, Molecular and cellular biology.

[11]  P. O’Connell,et al.  The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21 , 1990, Cell.

[12]  W. Schafer,et al.  Common modifications of trimeric G proteins and ras protein: involvement of polyisoprenylation. , 1990, Science.

[13]  M. Yamamoto,et al.  Role of a ras homolog in the life cycle of schizosaccharomyces pombe , 1986, Cell.

[14]  W. Heideman,et al.  Interactions between adenylate cyclase and the yeast GTPase-activating protein IRA1 , 1991, Molecular and cellular biology.

[15]  James R. Broach,et al.  SRV2, a gene required for RAS activation of adenylate cyclase in yeast , 1990, Cell.

[16]  F. McCormick,et al.  A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants. , 1987, Science.

[17]  M. Wigler,et al.  The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins , 1990, Cell.

[18]  M. Nakafuku,et al.  S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating protein , 1990, Cell.

[19]  D. Bar-Sagi,et al.  Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation , 1985, Cell.

[20]  S. Clarke,et al.  The Saccharomyces cerevisiae STE14 gene encodes a methyltransferase that mediates C‐terminal methylation of a‐factor and RAS proteins. , 1991, The EMBO journal.

[21]  R. Deschenes,et al.  Evidence for an S-farnesylcysteine methyl ester at the carboxyl terminus of the Saccharomyces cerevisiae RAS2 protein. , 1990, Biochemistry.

[22]  Margaret Robertson,et al.  The neurofibromatosis type 1 gene encodes a protein related to GAP , 1990, Cell.

[23]  P. Sternberg,et al.  let-60, a gene that specifies cell fates during C. elegans vulval induction, encodes a ras protein , 1990, Cell.

[24]  J. Broach,et al.  RAS genes in Saccharomyces cerevisiae: signal transduction in search of a pathway. , 1991, Trends in genetics : TIG.

[25]  J. François,et al.  The control of glycogen metabolism in yeast , 1988 .

[26]  M. Jacquet,et al.  Enhancement of the GDP-GTP exchange of RAS proteins by the carboxyl-terminal domain of SCD25. , 1990, Science.

[27]  M. Wigler,et al.  Mutations of the adenylyl cyclase gene that block RAS function in Saccharomyces cerevisiae. , 1990, Science.

[28]  A. Levitzki,et al.  In vitro reconstitution of cdc25 regulated S. cerevisiae adenylyl cyclase and its kinetic properties. , 1990, The EMBO journal.

[29]  A. Brown,et al.  ras p21 and GAP inhibit coupling of muscarinic receptors to atrial K+ channels , 1990, Cell.

[30]  F. Tamanoi,et al.  IRA2, an upstream negative regulator of RAS in yeast, is a RAS GTPase-activating protein. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[31]  K. Arai,et al.  Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein: studies on its structure and possible functions. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[32]  Frank McCormick,et al.  The GTPase superfamily: a conserved switch for diverse cell functions , 1990, Nature.

[33]  P. Casey,et al.  Inhibition of purified p21 ras farnesyl:protein transferase by Cys-AAX tetrapeptides , 1990, Cell.

[34]  J. Broach,et al.  The function of ras genes in Saccharomyces cerevisiae. , 1990, Advances in cancer research.

[35]  W. Heideman,et al.  Adenylate cyclase in Saccharomyces cerevisiae is a peripheral membrane protein , 1990, Molecular and cellular biology.

[36]  W. Schafer,et al.  Enzymatic coupling of cholesterol intermediates to a mating pheromone precursor and to the ras protein. , 1990, Science.

[37]  M. Wigler,et al.  Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase. , 1990, Molecular and cellular biology.

[38]  J. François,et al.  The control of glycogen metabolism in yeast. 1. Interconversion in vivo of glycogen synthase and glycogen phosphorylase induced by glucose, a nitrogen source or uncouplers. , 1988, European journal of biochemistry.

[39]  W. Schafer,et al.  Genetic and pharmacological suppression of oncogenic mutations in ras genes of yeast and humans. , 1989, Science.

[40]  M. Jacquet,et al.  The Saccharomyces cerevisiae CDC25 gene product is a 180 kDa polypeptide and is associated with a membrane fraction , 1990, FEBS letters.

[41]  K. Matsumoto,et al.  IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae , 1989, Molecular and cellular biology.

[42]  M. Wigler,et al.  The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway , 1987, Cell.

[43]  L. C. Robinson,et al.  CDC25: a component of the RAS-adenylate cyclase pathway in Saccharomyces cerevisiae. , 1987, Science.

[44]  T. Pollard,et al.  Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation. , 1991, Science.

[45]  M. Jacquet,et al.  The C-terminal part of the CDC25 gene product plays a key role in signal transduction in the glucose-induced modulation of cAMP level in Saccharomyces cerevisiae. , 1990, European journal of biochemistry.

[46]  M. Wigler,et al.  Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method , 1988, Molecular and cellular biology.

[47]  Frank McCormick,et al.  ras GTPase activating protein: Signal transmitter and signal terminator , 1989, Cell.

[48]  T. Kataoka,et al.  Leucine-rich repeats and carboxyl terminus are required for interaction of yeast adenylate cyclase with RAS proteins. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[49]  S. Carr,et al.  Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component. , 1988, The Journal of biological chemistry.

[50]  F. Tamanoi,et al.  The catalytic domain of the neurofibromatosis type 1 gene product stimulates ras GTPase and complements ira mutants of S. cerevisiae , 1990, Cell.