High correlation between skin color based on CIELAB color space, epidermal melanocyte ratio, and melanocyte melanin content

Background To treat skin color disorders, such as vitiligo or burns, melanocytes are transplanted for tissue regeneration. However, melanocyte distribution in the human body varies with age and location, making it difficult to select the optimal donor skin to achieve a desired color match. Determining the correlations with the desired skin color measurement based on CIELAB color, epidermal melanocyte numbers, and melanin content of individual melanocytes is critical for clinical application. Method Fifteen foreskin samples from Asian young adults were analyzed for skin color, melanocyte ratio (melanocyte proportion in the epidermis), and melanin concentration. Furthermore, an equation was developed based on CIELAB color with melanocyte ratio, melanin concentration, and the product of melanocyte ratio and melanin concentration. The equation was validated by seeding different ratios of keratinocytes and melanocytes in tissue-engineered skin substitutes, and the degree of fitness in expected skin color was confirmed. Results Linear regression analysis revealed a significant strong negative correlation (r = − 0.847, R2 = 0.717) between CIELAB L* value and the product of the epidermal melanocyte ratio and cell-based melanin concentration. Furthermore, the results showed that an optimal skin color match was achieved by the formula. Discussion We found that L* value was correlated with the value obtained from multiplying the epidermal melanocyte ratio (R) and melanin content (M) and that this correlation was more significant than either L* vs M or L* vs R. This suggests that more accurate prediction of skin color can be achieved by considering both R and M. Therefore, precise skin color match in treating vitiligo or burn patients would be potentially achievable based on extensive collection of skin data from people of Asian descent.

[1]  R. Kaufmann,et al.  Grafting of in vitro cultured melanocytes onto laser-ablated lesions in vitiligo. , 1998, Acta dermato-venereologica.

[2]  V. Hearing,et al.  Epidermal gene expression and ethnic pigmentation variations among individuals of Asian, European and African ancestry , 2014, Experimental dermatology.

[3]  N. Brankov,et al.  Pigmented basal cell carcinoma: increased melanin or increased melanocytes? , 2016, Journal of cutaneous pathology.

[4]  D. Greenhalgh,et al.  Pigmentation and microanatomy of skin regenerated from composite grafts of cultured cells and biopolymers applied to full-thickness burn wounds. , 1995, Transplantation.

[5]  A. Lerner,et al.  Pigment cell transplantation for treatment of vitiligo: a progress report. , 1994, Journal of the American Academy of Dermatology.

[6]  T. Fitzpatrick,et al.  Racial Differences in the Fate of Melanosomes in Human Epidermis , 1969, Nature.

[7]  S. Boyce,et al.  Regulation of cutaneous pigmentation by titration of human melanocytes in cultured skin substitutes grafted to athymic mice , 2002, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[8]  Shosuke Ito,et al.  Quantitative measures of the effect of the melanocortin 1 receptor on human pigmentary status. , 2004, The Journal of investigative dermatology.

[9]  H. Pinkus,et al.  Quantitative and qualitative data on the pigment cells of adult human epidermis. , 1957, The Journal of investigative dermatology.

[10]  S. Mulekar.  Melanocyte–keratinocyte cell transplantation for stable vitiligo , 2003, International journal of dermatology.

[11]  N. van Geel,et al.  Modified Technique of Autologous Noncultured Epidermal Cell Transplantation for Repigmenting Vitiligo: A Pilot Study , 2001, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[12]  Y. Gauthier,et al.  Non-cultured epidermal suspension in vitiligo: from laboratory to clinic. , 2012, Indian journal of dermatology, venereology and leprology.

[13]  M. Olsson,et al.  The melanogenesis and mechanisms of skin‐lightening agents – existing and new approaches , 2011, International journal of cosmetic science.

[14]  S. Hoath,et al.  The organization of human epidermis: functional epidermal units and phi proportionality. , 2003, The Journal of investigative dermatology.

[15]  A. M. Kahn,et al.  Vitiligo: treatment by dermabrasion and epithelial sheet grafting. , 1995, Journal of the American Academy of Dermatology.

[16]  Sanjeev Gupta,et al.  Modified technique of suction blistering for epidermal grafting in vitiligo , 1999, International journal of dermatology.

[17]  R. Snell,et al.  THE MELANOCYTES AND MELANIN IN HUMAN ABDOMINAL WALL SKIN: A SURVEY MADE AT DIFFERENT AGES IN BOTH SEXES AND DURING PREGNANCY. , 1963, Journal of anatomy.

[18]  D. Didona,et al.  Vitiligo: Pathogenesis, clinical variants and treatment approaches. , 2016, Autoimmunity reviews.

[19]  H. Pinkus,et al.  Quantitative and Qualitative Data on the Pigment Cells of Adult Human Epidermis1 , 1957 .

[20]  T. Fitzpatrick,et al.  The evolution of concepts of melanin biology. , 1967, Archives of dermatology.

[21]  S. Boyce,et al.  Regulation of pigmentation in cultured skin substitutes by cytometric sorting of melanocytes and keratinocytes. , 1997, The Journal of investigative dermatology.

[22]  S. Alaluf,et al.  Ethnic variation in melanin content and composition in photoexposed and photoprotected human skin. , 2002, Pigment cell research.

[23]  I. Weatherall,et al.  Skin color measurements in terms of CIELAB color space values. , 1992, The Journal of investigative dermatology.

[24]  D. Hu,et al.  Treatment of vitiligo by transplantation of cultured pure melanocyte suspension: analysis of 120 cases. , 2004, Journal of the American Academy of Dermatology.

[25]  J. Naeyaert,et al.  Modified technique of autologous noncultured epidermal cell transplantation for repigmenting vitiligo: a pilot study. , 2001 .

[26]  R. Falabella.  Surgical therapies for vitiligo and other leukodermas, part 1: minigrafting and suction epidermal grafting , 2001 .

[27]  L. Juhlin,et al.  Long‐term follow‐up of leucoderma patients treated with transplants of autologous cultured melanocytes, ultrathin epidermal sheets and basal cell layer suspension , 2002, The British journal of dermatology.

[28]  Daegu Son,et al.  Skin color of Koreans: statistical evaluation of affecting factors , 2006, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[29]  C. Cohen,et al.  Key Regulatory Role of Dermal Fibroblasts in Pigmentation as Demonstrated Using a Reconstructed Skin Model: Impact of Photo-Aging , 2014, PloS one.

[30]  Timothy J. Lavelle,et al.  Understanding the Melanocyte Distribution in Human Epidermis: An Agent-Based Computational Model Approach , 2012, PloS one.

[31]  D. Cox,et al.  A genomewide association study of skin pigmentation in a South Asian population. , 2007, American journal of human genetics.

[32]  Philippe Bastien,et al.  Chemical analysis of constitutive pigmentation of human epidermis reveals constant eumelanin to pheomelanin ratio , 2015, Pigment cell & melanoma research.

[33]  I. Weatherall,et al.  A comparison of two different instruments for measuring venison CIELAB values and colour assessment by a trained panel , 1991 .

[34]  T. Dadd,et al.  NCKX5, a natural regulator of human skin colour variation, regulates the expression of key pigment genes MC1R and alpha-MSH and alters cholesterol homeostasis in normal human melanocytes. , 2013, Advances in experimental medicine and biology.

[35]  Y. Tsuchida,et al.  The Correlation of Skin Blood Flow with Age, Total Cholesterol, Hematocrit, Blood Pressure, and Hemoglobin , 1991, Plastic and Reconstructive Surgery.

[36]  S. Alaluf,et al.  The impact of epidermal melanin on objective measurements of human skin colour. , 2002, Pigment cell research.

[37]  Shosuke Ito,et al.  Diversity of pigmentation in cultured human melanocytes is due to differences in the type as well as quantity of melanin. , 2006, Pigment cell research.

[38]  Yi-Wen Wang,et al.  Restoration of skin pigmentation after deep partial or full-thickness burn injury. , 2018, Advanced drug delivery reviews.

[39]  A. Coombes,et al.  Composite cell support membranes based on collagen and polycaprolactone for tissue engineering of skin. , 2004, Biomaterials.