Research on dynamic holographic microscopic imaging method for deep-sea in situ detection

In order to realize the in situ detection of plankton and organic particles in the deep sea, we proposed a dynamic holographic microscopic imaging method based on the principle of parallel phase-shifting. We analyzed the implementation mechanism of the parallel phase-shifting holographic imaging. We designed the implementation scheme of the deep sea in situ detection system, carrying out experimental research on underwater dynamic holography. High resolution images were acquired and reconstructed using the resolution target and several biological samples. Experimental results show that local details of underwater samples can be clearly distinguished. We preliminarily verified the feasibility of the parallel phaseshifting holographic method to achieve underwater high-resolution in situ detection.