论文信息 - In vivo imaging of bone micro-architecture in mice with 3D synchrotron radiation micro-tomography

In vivo imaging of bone micro-architecture in mice with 3D synchrotron radiation micro-tomography

Abstract Micro-tomographic imaging of bone micro-architecture is increasingly used on wild and transgenic mice to follow effects of diseases or therapeutics. Synchrotron radiation micro-tomography (SR micro-CT) provides quantitative images at very high spatial resolution but has mainly been used in vitro. The aim of this work was to show the feasibility of SR micro-CT for assessing bone mineral density and micro-architecture in vivo in mice. Imaging with a pixel size of 10 μm was performed on beam line ID19 at the ESRF using a special mouse holder. Two strains of mice (C3H/HeJ and C57BL/6J) were used for the experiment. First tests were performed in order to optimize the imaging conditions with respect to dose. Then, six mice of each group were imaged at doses of 7 and 13 Gy (total scan time

[1] H Weinans,et al. Detecting and tracking local changes in the tibiae of individual rats: a novel method to analyse longitudinal in vivo micro-CT data. , 2004, Bone.

[2] F Peyrin,et al. A method for the automatic characterization of bone architecture in 3D mice microtomographic images. , 2003, Computerized Medical Imaging and Graphics.

[3] Laurence Vico,et al. Tail Suspension Induces Bone Loss in Skeletally Mature Mice in the C57BL/6J Strain but Not in the C3H/HeJ Strain , 2003, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[4] J. Kinney,et al. In vivo, three‐dimensional microscopy of trabecular bone , 1995, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[5] Françoise Peyrin,et al. Quantification of the degree of mineralization of bone in three dimensions using synchrotron radiation microtomography. , 2002, Medical physics.

[6] M J Welch,et al. Small animal imaging: current technology and perspectives for oncological imaging , 2002 .

[7] P Cloetens,et al. A synchrotron radiation microtomography system for the analysis of trabecular bone samples. , 1999, Medical physics.