3D Reconstruction of Histological Rat Brain Images

Histology of brain sections provides the opportunity to analyse the brain structure on a microscopic level. Histological sections have to be aligned into a 3D reference volume to obtain a correct spatial localization of cell structures. This paper presents a new approach of a rigid registration method of histological sections to a blockface volume. The method and the results are validated by using synthetic data and experimental histological rat brain images. The results indicate a robust, simple and valid image registration method.