Inhibitors of Plasminogen Activation in Human Blood

Plasma contained two inhibitors of plasminogen activation by urokinase when fractioned by gel filtration on Sephadex G‐200. The inhibitor in the lower molecular weight fractions was separate from the principal protease inhibitors of plasma: α1‐antitrypsin, α2‐macroglobulin, C1 inactivator and antithrombin III, and from factor XIII. This activation inhibitor was present in both plasma and serum and its recovery was not reduced by preincubating the serum for 6 h at 37°C. Its inhibitory activity was stable for several days at 4°C, and was enhanced in the presence of an increased saline concentration. Preparations of the inhibitor, active in a clot lysis system, failed to inhibit the esterase activity of urokinase on N‐α‐acetylglycyl‐L‐lysine methyl ester.

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