Protein Purification: Principles and Practice

Prefaces 1. The Protein Purification Laboratory 2. Making an Extract 3. Analysis- Measurement of Protein and Enzyme Activity 4. Separation by Precipitation 5. Separation by Adsorption I: General Principles 6. Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents 7. Separation by Adsorption: Affinity Techniques 8. Separation in Solution 9. Purification of Special Types of Proteins 10. Small-Scale and Large-Scale Procedures 11. Analysis for Purity 12. Optimization of Procedures: Final Steps Appendix A-D References Index