Enzymes produced by bacteria within biofilms of dairy origin and their effect on dairy products : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy, Massey University, Palmerston North, New Zealand

Content published as: Teh, K.H., Flint, S., Palmer, J., Lindsay, D., Andrewes, P., & Bremer, P. (2011). Thermo-resistant enzyme-producing bacteria isolated from the internal surfaces of raw milk tankers. International Dairy Journal, 21(10), 742-747. doi: 10.1016/j.idairyj.2011.04.013. .(chapter 3b). Teh, K.H., Flint, S., Palmer, J., Andrewes, P., Bremer, P. & Lindsay, D. (2012). Proteolysis produced within biofilms of bacterial isolates from raw milk tankers. International Journal of Food Microbiology, 157, 28-34. doi: 10.1016/j.ijfoodmicro.2012.04.008. (chapter 4a). Teh, K.H., Lindsay, D., Palmer, J., Andrewes, P., Bremer, P., & Flint, S. (2013). Lipolysis within single culture and co-culture biofilms of dairy origin. International Journal of Food Microbiology, 163(2-3), 129-135. doi: 10.1016/j.ijfoodmicro.2013.02.009. (chapter 4b)

[1]  M. Smeltzer,et al.  Quantitative spectrophotometric assay for staphylococcal lipase , 1992, Applied and environmental microbiology.

[2]  C. Callon,et al.  Characteristics of microbial biofilm on wooden vats ('gerles') in PDO Salers cheese. , 2012, International journal of food microbiology.

[3]  B. G. Lovegrove,et al.  Modification and miniaturization of Thermochron iButtons for surgical implantation into small animals , 2009, Journal of Comparative Physiology B.

[4]  Matthew R. Johnson,et al.  Transcriptional Analysis of Biofilm Formation Processes in the Anaerobic, Hyperthermophilic Bacterium Thermotoga maritima , 2004, Applied and Environmental Microbiology.

[5]  M. Griffiths,et al.  Psychrotrophs in dairy products: their effects and their control. , 1994, Critical reviews in food science and nutrition.

[6]  F. Villani,et al.  Protease and esterase activity of staphylococci. , 2006, International journal of food microbiology.

[7]  M. L. Cabo,et al.  Effects of mussel processing soils on the adherence of Listeria monocytogenes to polypropylene and stainless steel. , 2009, Journal of food protection.

[8]  F. Hellweger,et al.  A bunch of tiny individuals—Individual-based modeling for microbes , 2009 .

[9]  John D. Brooks,et al.  Properties of the stainless steel substrate, influencing the adhesion of thermo-resistant streptococci , 2000 .

[10]  S. Nielsen,et al.  The effect of raw milk storage temperature on plasmin activity and plasminogen activation in pasteurized milk , 2008 .

[11]  J. Saiter,et al.  Adhesion of Yersinia ruckeri to fish farm materials: influence of cell and material surface properties , 2002 .

[12]  S. Swift,et al.  Quorum Sensing-Dependent Regulation and Blockade of Exoprotease Production in Aeromonas hydrophila , 1999, Infection and Immunity.

[13]  F. Tanaka,et al.  Control of milk pH reduces biofilm formation of Bacillus licheniformis and Lactobacillus paracasei on stainless steel , 2012 .

[14]  M. Vieira,et al.  Characterization of Contaminants from a Sanitized Milk Processing Plant , 2012, PloS one.

[15]  B. Zhang,et al.  Extracellular enzyme activities during regulated hydrolysis of high-solid organic wastes. , 2007, Water research.

[16]  A. Kilara,et al.  Hydrolysis of Milk Fat By Lipase in Solvent‐free Phospholipid Reverse Micellar Media , 1996 .

[17]  Tslil Ophir,et al.  A Role for Exopolysaccharides in the Protection of Microorganisms from Desiccation , 1994, Applied and environmental microbiology.

[18]  A. Bakhrouf,et al.  A microtiter plate assay for Staphylococcus aureus biofilm quantification at various pH levels and hydrogen peroxide supplementation. , 2010, The new microbiologica.

[19]  M. Hentzer,et al.  Dynamics and Spatial Distribution of β-Lactamase Expression in Pseudomonas aeruginosa Biofilms , 2004, Antimicrobial Agents and Chemotherapy.

[20]  M. Johansson,et al.  Protein degradation in bovine milk caused by Streptococcus agalactiae , 2012, Journal of Dairy Research.

[21]  P. de Vos,et al.  Heterogeneity of heat-resistant proteases from milk Pseudomonas species. , 2009, International journal of food microbiology.

[22]  F. Siñeriz,et al.  Cell immobilization for production of lactic acid biofilms do it naturally. , 2010, Advances in applied microbiology.

[23]  M. Turner,et al.  Role of attachment to surfaces on the prevalence and survival of Campylobacter through food systems. , 2012, Journal of food protection.

[24]  M. Vieira,et al.  Potential of the adhesion of bacteria isolated from drinking water to materials , 2007, Journal of basic microbiology.

[25]  J. Frank,et al.  A predictive model for heat inactivation of Listeria monocytogenes biofilm on stainless steel. , 2004, Journal of food protection.

[26]  C. Alvarado,et al.  Attachment of Salmonella serovars and Listeria monocytogenes to stainless steel and plastic conveyor belts. , 2012, Poultry science.

[27]  J. Remon,et al.  Kinetics of Pseudomonas aeruginosa adhesion to 304 and 316-L stainless steel: role of cell surface hydrophobicity , 1990, Applied and environmental microbiology.

[28]  A. Brandelli,et al.  A psychrotrophic Burkholderia cepacia strain isolated from refrigerated raw milk showing proteolytic activity and adhesion to stainless steel. , 2011, The Journal of dairy research.

[29]  S. Cuppett,et al.  Lipase production by lactic acid bacteria and activity on butter oil , 1996 .

[30]  Koon Hoong Teh,et al.  Lipolysis within single culture and co-culture biofilms of dairy origin. , 2013, International journal of food microbiology.

[31]  P. de Vos,et al.  Influence of Storage Conditions on the Growth of Pseudomonas Species in Refrigerated Raw Milk , 2010, Applied and Environmental Microbiology.

[32]  O. Mills,et al.  Detection of lipase in skim and whole milk powders using triheptanoin as a substrate , 2007 .

[33]  F. Hasan,et al.  Methods for detection and characterization of lipases: A comprehensive review. , 2009, Biotechnology advances.

[34]  K. Hashimoto,et al.  Optimum conditions for the production of lipase by alginate-immobilized bacteria. , 1992 .

[35]  E. M. Brown,et al.  Review of the chemistry of alphaS2-casein and the generation of a homologous molecular model to explain its properties. , 2009, Journal of dairy science.

[36]  M. Schembri,et al.  Global gene expression in Escherichia coli biofilms , 2003, Molecular microbiology.

[37]  Bernd Bendinger,et al.  Physicochemical Cell Surface and Adhesive Properties of Coryneform Bacteria Related to the Presence and Chain Length of Mycolic Acids , 1993, Applied and environmental microbiology.

[38]  S. Kjelleberg,et al.  A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms , 2006, Molecular microbiology.

[39]  C. Keevil,et al.  Influence of temperature and plumbing material selection on biofilm formation and growth of Legionella pneumophila in a model potable water system containing complex microbial flora , 1994, Applied and environmental microbiology.

[40]  Steve Flint,et al.  Bacterial cell attachment, the beginning of a biofilm , 2007, Journal of Industrial Microbiology & Biotechnology.

[41]  D. Mollé,et al.  Effects of storage temperature on physico-chemical characteristics of semi-skimmed UHT milk , 2008 .

[42]  H. Deeth.  Lipoprotein lipase and lipolysis in milk , 2006 .

[43]  K. Veeraragavan.  A simple and sensitive method for the estimation of microbial lipase activity. , 1990, Analytical biochemistry.

[44]  J. Karns,et al.  Biofilm in milking equipment on a dairy farm as a potential source of bulk tank milk contamination with Listeria monocytogenes. , 2010, Journal of dairy science.

[45]  D. Evans,et al.  Formation and dispersal of bacterial biofilms in vivo and in situ. , 1993, The Journal of applied bacteriology.

[46]  J. Wallach,et al.  Conductimetric Assay of a Bacterial Lipase, Using Triacetin as a Substrate , 1982 .

[47]  I. Sutherland.  Biofilm exopolysaccharides: a strong and sticky framework. , 2001, Microbiology.

[48]  Koon Hoong Teh,et al.  Thermo-resistant enzyme-producing bacteria isolated from the internal surfaces of raw milk tankers , 2011 .

[49]  D M Barbano,et al.  Influence of raw milk quality on fluid milk shelf life. , 2006, Journal of dairy science.

[50]  P. Kingshott,et al.  Adhesion of food‐borne bacteria to stainless steel is reduced by food conditioning films , 2009, Journal of applied microbiology.

[51]  A. Grossman,et al.  Identification of AbrB‐regulated genes involved in biofilm formation by Bacillus subtilis , 2004, Molecular microbiology.

[52]  N. Gupta,et al.  Lipase assays for conventional and molecular screening: an overview , 2003, Biotechnology and applied biochemistry.

[53]  L. Barnes,et al.  Effect of Milk Proteins on Adhesion of Bacteria to Stainless Steel Surfaces , 1999, Applied and Environmental Microbiology.

[54]  G. Mazza,et al.  Detection and measurement of microbial lipase activity: a review. , 1999, Critical reviews in food science and nutrition.

[55]  T. Huppertz.  Foaming properties of milk: A review of the influence of composition and processing , 2010 .

[56]  F. Gasperi,et al.  Does milk treatment before cheesemaking affect microbial and chemical traits of ripened cheese? Grana Trentino as a case study. , 2012, Journal of dairy science.

[57]  G. B. Wisdom,et al.  Effect of quorum sensing agents on the growth kinetics of Pseudomonas spp. of raw milk origin , 2005, Journal of Dairy Research.

[58]  F. Tanaka,et al.  Surface conditioning of stainless steel coupons with skim milk solutions at different pH values and its effect on bacterial adherence , 2010 .

[59]  P. Fox,et al.  Indigenous proteolytic enzymes in milk: A brief overview of the present state of knowledge , 2006 .

[60]  J. Kreft,et al.  Interaction of Listeria monocytogenes and Staphylococcus epidermidis in dual species biofilms. , 2010 .

[61]  R. López-Fandiño,et al.  Assessment of the quality of dairy products by capillary electrophoresis of milk proteins. , 1997, Journal of chromatography. B, Biomedical sciences and applications.

[62]  P. de Vos,et al.  Incidence and Diversity of Potentially Highly Heat-Resistant Spores Isolated at Dairy Farms , 2005, Applied and Environmental Microbiology.

[63]  I. Gentle,et al.  Surface roughness of stainless steel influences attachment and detachment of Escherichia coli O157. , 2011, Journal of food protection.

[64]  Nuno F. Azevedo,et al.  Time to “go large” on biofilm research: advantages of an omics approach , 2009, Biotechnology Letters.

[65]  J. W. Arnold,et al.  Comparison of poultry processing equipment surfaces for susceptibility to bacterial attachment and biofilm formation. , 2000, Poultry science.

[66]  John D. Brooks,et al.  Biofilms in dairy manufacturing plant‐description, current concerns and methods of control , 1997 .

[67]  G. Stewart,et al.  Lipolytic Esterases in Staphylococci , 1968, Journal of bacteriology.

[68]  H. Deeth,et al.  A sensitive HPLC method for measuring bacterial proteolysis and proteinase activity in UHT milk , 2006 .

[69]  Zhi-Wu Wang,et al.  Potential of biofilm-based biofuel production , 2009, Applied Microbiology and Biotechnology.

[70]  J. Frank,et al.  Proteolysis of High Quality Raw Milk During Storage at Various Temperatures , 1988 .

[71]  K. Ito,et al.  Purification and characterization of extracellular and cell wall bound beta-glucosidases from Aspergillus kawachii. , 1998, Bioscience, biotechnology, and biochemistry.

[72]  W. Holzapfel,et al.  The influence of extrinsic factors on the microbiological spoilage pattern of ground beef. , 1988, International journal of food microbiology.

[73]  R. Daniel,et al.  Detection and impact of protease and lipase activities in milk and milk powders , 2003 .

[74]  G. Norris,et al.  Esterases of lactic acid bacteria and cheese flavour: Milk fat hydrolysis, alcoholysis and esterification , 2005 .

[75]  R. Kolter,et al.  Biofilm formation as microbial development. , 2000, Annual review of microbiology.

[76]  F. Laurent,et al.  Effects of storage conditions on the composition of raw milk , 1995 .

[77]  A. Kilara,et al.  Peptides From Milk Proteins and Their Properties , 2003, Critical reviews in food science and nutrition.

[78]  P. Bremer,et al.  The resistance to heat of thermo-resistant streptococci attached to stainless steel in the presence of milk , 2002, Journal of Industrial Microbiology and Biotechnology.

[79]  A. Pometto,et al.  Ligninolytic enzyme production by Phanerochaete chrysosporium in plastic composite support biofilm stirred tank bioreactors. , 2006, Journal of agricultural and food chemistry.

[80]  P. Holden,et al.  Bacterial and Mineral Elements in an Arctic Biofilm: A Correlative Study Using Fluorescence and Electron Microscopy , 2010, Microscopy and Microanalysis.

[81]  T. Oh,et al.  Biochemical properties and substrate specificity of lipase from Staphylococcus aureus B56 , 2002 .

[82]  D. Lindsay,et al.  Cleaning and handling implements as potential reservoirs for bacterial contamination of some ready-to-eat foods in retail delicatessen environments. , 2007, Journal of food protection.

[83]  P. Laurent,et al.  Temperature dependence of extracellular enzymes production by psychrotrophic and psychrophilic bacteria , 2000, Biotechnology Letters.

[84]  L. Cellini,et al.  Effect of alkaline pH on staphylococcal biofilm formation , 2012, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[85]  K. Seo,et al.  Attachment of Escherichia coli O157:H7 to lettuce leaf surface and bacterial viability in response to chlorine treatment as demonstrated by using confocal scanning laser microscopy. , 1999, Journal of food protection.

[86]  A. Chopra,et al.  Purification and characterization of heat-stable proteases from Bacillus stearothermophilus RM-67. , 1985, Journal of dairy science.

[87]  E. A. Zottola,et al.  Isolation and Identification of Adherent Gram-Negative Microorganisms from Four Meat-Processing Facilities. , 1997, Journal of food protection.

[88]  J. Durst,et al.  The survival of Listeria monocytogenes. , 1972 .

[89]  Korin E. Wheeler,et al.  Characterization of Extracellular Polymeric Substances from Acidophilic Microbial Biofilms , 2010, Applied and Environmental Microbiology.

[90]  E. Somers,et al.  Biofilm formation and contamination of cheese by nonstarter lactic acid bacteria in the dairy environment. , 2001, Journal of dairy science.

[91]  F. Leriche,et al.  No seasonal effect on culturable pseudomonads in fresh milks from cattle herds. , 2012, Journal of dairy science.

[92]  Novel method for the proteomic investigation of a dairy-associated Bacillus cereus biofilm. , 2001, FEMS microbiology letters.

[93]  F. Ergan,et al.  Simple high performance liquid chromatography methods for monitoring lipase reactions , 2006, Lipids.

[94]  S. K. Garg.  Psychrotrophs in milk - a review. , 1990 .

[95]  F. Götz,et al.  Staphylococcal lipases: biochemical and molecular characterization. , 2000, Biochimie.

[96]  G. Storz,et al.  A small RNA that regulates motility and biofilm formation in response to changes in nutrient availability in Escherichia coli , 2012, Molecular microbiology.

[97]  S. Flint,et al.  Thermophilic bacilli and their importance in dairy processing. , 2010, International journal of food microbiology.

[98]  S. Dharmsthiti,et al.  Production, purification and characterization of thermophilic lipase from Bacillus sp. THL027. , 1999, FEMS microbiology letters.

[99]  M. Griffiths,et al.  Thermostability of proteases and lipases from a number of species of psychrotrophic bacteria of dairy origin. , 1981, The Journal of applied bacteriology.

[100]  A. De Bartolomeo,et al.  Numerical analysis and DNA base compositions of some thermophilic Bacillus species. , 1991, International journal of systematic bacteriology.

[101]  P. Vandamme,et al.  PCR-Based Assay for Differentiation of Pseudomonas aeruginosa from Other Pseudomonas Species Recovered from Cystic Fibrosis Patients , 2004, Journal of Clinical Microbiology.

[102]  J. Costerton,et al.  The involvement of cell-to-cell signals in the development of a bacterial biofilm. , 1998, Science.

[103]  J. Sha,et al.  N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. , 2009, Microbiology.

[104]  J. Gaillard,et al.  Extracellular protease activity of different Pseudomonas strains: dependence of proteolytic activity on culture conditions , 2005, Journal of applied microbiology.

[105]  R. Rajendran,et al.  Hydrolytic Enzyme Production is Associated with Candida Albicans Biofilm Formation from Patients with Type 1 Diabetes , 2010, Mycopathologia.

[106]  G. K. Villena,et al.  Cellulase production by Aspergillus niger in biofilm, solid-state, and submerged fermentations , 2010, Applied Microbiology and Biotechnology.

[107]  K. Ito,et al.  Extracellular soluble polysaccharide (ESP) from Aspergillus kawachii improves the stability of extracellular beta-gluocosidases (EX-1 and EX-2) and is involved in their localization. , 2001, Journal of bioscience and bioengineering.

[108]  Xianming Shi,et al.  Biofilm formation and food safety in food industries , 2009 .

[109]  R. Fischetti,et al.  Comparison of different biochemical and molecular methods for the identification of Vibrio parahaemolyticus , 2007, Journal of applied microbiology.

[110]  W. C. Frazier,et al.  STUDIES ON THE PROTEOLYTIC BACTERIA OF MILK III. ACTION OF PROTEOLYTIC BACTERIA OF MILK ON CASEIN AND GELATIN , 1928, Journal of bacteriology.

[111]  R. K. Saxena,et al.  MICROBIAL LIPASES : POTENTIAL BIOCATALYSTS FOR THE FUTURE INDUSTRY , 1999 .

[112]  A. Brandelli,et al.  Proteolytic activity among psychrotrophic bacteria isolated from refrigerated raw milk , 2010 .

[113]  L. Skovgaard,et al.  Rapid development in vitro and in vivo of resistance to ceftazidime in biofilm‐growing Pseudomonas aeruginosa due to chromosomal β‐lactamase , 2000, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[114]  L. McLandsborough,et al.  Effect of surface roughness and stainless steel finish on Listeria monocytogenes attachment and biofilm formation. , 2008, Journal of food protection.

[115]  J. Frank,et al.  Removal of Pseudomonas putida biofilm and associated extracellular polymeric substances from stainless steel by alkali cleaning. , 2005, Journal of food protection.

[116]  A. Bakhrouf,et al.  Adhesive ability and biofilm metabolic activity of Listeria monocytogenes strains before and after cold stress , 2012 .

[117]  G. Donelli,et al.  Microbial Biofilms , 2014, Methods in Molecular Biology.

[118]  F. Villani,et al.  Simultaneous Detection of Pseudomonas fragi, P. lundensis, and P. putida from Meat by Use of a Multiplex PCR Assay Targeting the carA Gene , 2007, Applied and Environmental Microbiology.

[119]  J. Frank,et al.  Surface-adherent Growth of Listeria monocytogenes is Associated with Increased Resistance to Surfactant Sanitizers and Heat. , 1990, Journal of food protection.

[120]  V. Hájek.  Staphylococcus intermedius, a New Species Isolated from Animals , 1976 .

[121]  A. Gruss,et al.  Raw Cow Milk Bacterial Population Shifts Attributable to Refrigeration , 2004, Applied and Environmental Microbiology.

[122]  D. van den Broek,et al.  The role of phenotypic variation in rhizosphere Pseudomonas bacteria. , 2005, Environmental microbiology.

[123]  G. Nychas,et al.  Attachment and biofilm formation by Escherichia coli O157:H7 at different temperatures, on various food-contact surfaces encountered in beef processing. , 2011, International journal of food microbiology.

[124]  E. Bartowsky,et al.  Acetic acid bacteria spoilage of bottled red wine -- a review. , 2008, International journal of food microbiology.

[125]  L. Ju,et al.  Initial bacterial attachment in slow flowing systems: effects of cell and substrate surface properties. , 2011, Colloids and surfaces. B, Biointerfaces.

[126]  J. Struthers,et al.  Interaction of Streptococcus pneumoniae andMoraxella catarrhalis: Investigation of the Indirect Pathogenic Role of β-Lactamase-Producing Moraxellae by Use of a Continuous-Culture Biofilm System , 1998, Antimicrobial Agents and Chemotherapy.

[127]  R. Rocha,et al.  Genetic diversity of Gram-negative, proteolytic, psychrotrophic bacteria isolated from refrigerated raw milk. , 2006, International journal of food microbiology.

[128]  P. Fox,et al.  Indigenous enzymes in milk: Overview and historical aspects—Part 1 , 2006 .

[129]  J. Membré,et al.  Effects of Temperature, pH, and NaCl on Growth and Pectinolytic Activity of Pseudomonas marginalis , 1994, Applied and environmental microbiology.

[130]  L. De Vuyst,et al.  Biochemical properties of Streptococcus macedonicus strains isolated from Greek Kasseri cheese , 2000, Journal of Applied Microbiology.

[131]  D. Barbano,et al.  Sensory threshold of off-flavors caused by proteolysis and lipolysis in milk. , 2003, Journal of dairy science.

[132]  T. Sørhaug,et al.  Psychrotrophs and their enzymes in milk and dairy products: Quality aspects , 1997 .

[133]  Guyot,et al.  Preliminary characterization of microflora of Comté cheese , 1998, Journal of applied microbiology.

[134]  M. Lewis,et al.  Comparison of methods for analysis of proteolysis by plasmin in milk. , 2011, The Journal of dairy research.

[135]  Everett Shock,et al.  Merging Genomes with Geochemistry in Hydrothermal Ecosystems , 2002, Science.

[136]  H. Roginski,et al.  The effects of refrigerated storage of raw milk on the quality of whole milk powder stored for different periods , 1997 .

[137]  M. Spector,et al.  Resistance and survival strategies of Salmonella enterica to environmental stresses , 2012 .

[138]  S. Molin,et al.  Long-Term Succession of Structure and Diversity of a Biofilm Formed in a Model Drinking Water Distribution System , 2003, Applied and Environmental Microbiology.

[139]  G. O’Toole,et al.  Alpha-Toxin Is Required for Biofilm Formation by Staphylococcus aureus , 2003, Journal of bacteriology.

[140]  K. Boor,et al.  Tracking heat-resistant, cold-thriving fluid milk spoilage bacteria from farm to packaged product. , 2008, Journal of dairy science.

[141]  P. Fox,et al.  Milk alkaline proteinase , 1988, Journal of Dairy Research.

[142]  P. de Vos,et al.  Seasonal influence on heat-resistant proteolytic capacity of Pseudomonas lundensis and Pseudomonas fragi, predominant milk spoilers isolated from Belgian raw milk samples. , 2009, Environmental microbiology.

[143]  D. Abernethy,et al.  Total Protein Methods and Their Potential Utility to Reduce the Risk of Food Protein Adulteration. , 2010, Comprehensive reviews in food science and food safety.

[144]  P. McSweeney,et al.  Advances in the study of proteolysis during cheese ripening , 2001 .

[145]  M. Galbe,et al.  Bio-ethanol--the fuel of tomorrow from the residues of today. , 2006, Trends in biotechnology.

[146]  M. Vieira,et al.  Physico-chemical surface characterization of a bacterial population isolated from a milking machine , 2005 .

[147]  G. Dimitracopoulos,et al.  Comparison of two commercial methods with PCR restriction fragment length polymorphism of the tuf gene in the identification of coagulase‐negative staphylococci , 2006, Letters in applied microbiology.

[148]  P. Bremer,et al.  Laboratory scale Clean-In-Place (CIP) studies on the effectiveness of different caustic and acid wash steps on the removal of dairy biofilms. , 2006, International journal of food microbiology.

[149]  A. Coorevits,et al.  Biofilm formation in milk production and processing environments: influence on milk quality and safety , 2012 .

[150]  Shinya Matsumoto,et al.  Bacterial adhesion: From mechanism to control , 2010 .

[151]  D. Fairbairn,et al.  Proteinases of psychrotrophic bacteria: their production, properties, effects and control , 1986, Journal of Dairy Research.

[152]  L. Vorlová,et al.  Effect of Bacillus cereus Enzymes on Milk Quality following Ultra High Temperature Processing , 2006 .

[153]  E. Abad,et al.  RFID smart tag for traceability and cold chain monitoring of foods: Demonstration in an intercontinental fresh fish logistic chain , 2009 .

[154]  C. Dodd,et al.  Susceptibility of suspended and surface-attached Salmonella enteritidis to biocides and elevated temperatures , 1995, Applied and environmental microbiology.

[155]  E. A. Zottola,et al.  Biofilm Formation by Listeria monocytogenes Utilizes a Primary Colonizing Microorganism in Flowing Systems. , 1993, Journal of food protection.

[156]  Roberta Bussamara,et al.  Isolation of a lipase-secreting yeast for enzyme production in a pilot-plant scale batch fermentation. , 2010, Bioresource technology.

[157]  U. Szewzyk,et al.  Bacterial extracellular DNA forming a defined network-like structure. , 2006, FEMS microbiology letters.

[158]  J. Costerton,et al.  Interspecies bacterial interactions in biofilms , 1995, Journal of Industrial Microbiology.

[159]  L. Bava,et al.  Effect of cleaning procedure and hygienic condition of milking equipment on bacterial count of bulk tank milk. , 2011, The Journal of dairy research.

[160]  Ehud Banin,et al.  Multi-species biofilms: living with friendly neighbors. , 2012, FEMS microbiology reviews.

[161]  G. R. Castro,et al.  Production of amylolytic enzymes by Bacillus amyloliquefaciens in pure culture and in co-culture with Zymomonas mobilis , 1999, Biotechnology Letters.

[162]  Bernhard Hauer,et al.  Microbial biofilms: a concept for industrial catalysis? , 2009, Trends in biotechnology.

[163]  Y. Missirlis,et al.  Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions. , 2004, European cells & materials.

[164]  G. O’Toole,et al.  Microbial Biofilms: from Ecology to Molecular Genetics , 2000, Microbiology and Molecular Biology Reviews.

[165]  P. Nielsen,et al.  Enzymatic activity in the activated-sludge floc matrix , 1995, Applied Microbiology and Biotechnology.

[166]  L. Gal,et al.  Biofilm-detached cells, a transition from a sessile to a planktonic phenotype: a comparative study of adhesion and physiological characteristics in Pseudomonas aeruginosa. , 2008, FEMS microbiology letters.

[167]  H. Roginski,et al.  Reconstituted UHT-treated milk: Effects of raw milk, powder quality and storage conditions of UHT milk on its physico-hemical attributes and flavour , 1997 .

[168]  S Krawiec,et al.  Temperature characteristics and Arrhenius plots for nominal psychrophiles, mesophiles and thermophiles. , 1980, Journal of general microbiology.

[169]  Uwe Schröder,et al.  Electroactive mixed culture biofilms in microbial bioelectrochemical systems: the role of temperature for biofilm formation and performance. , 2010, Biosensors & bioelectronics.

[170]  P. Alifano,et al.  Efficacy of silver treated catheters for haemodialysis in preventing bacterial adhesion , 2012, Journal of Materials Science: Materials in Medicine.

[171]  Hans-Curt Flemming,et al.  The EPS Matrix: The “House of Biofilm Cells” , 2007, Journal of bacteriology.

[172]  A. Klibanov,et al.  On the issue of interfacial activation of lipase in nonaqueous media , 1996, Biotechnology and bioengineering.

[173]  S. J. Billington,et al.  Characterization of Campylobacter jejuni Biofilms under Defined Growth Conditions , 2007, Applied and Environmental Microbiology.

[174]  P. Ribereau-gayon,et al.  Evolution of Acetic Acid Bacteria During Fermentation and Storage of Wine , 1984, Applied and environmental microbiology.

[175]  Brigitte Petersen,et al.  Temperature monitoring in meat supply chains , 2011 .

[176]  Koon Hoong Teh,et al.  Proteolysis produced within biofilms of bacterial isolates from raw milk tankers. , 2012, International journal of food microbiology.

[177]  B. Law,et al.  The effect of lipolytic Gram-negative psychrotrophs in stored milk on the development of rancidity in Cheddar cheese , 1976, Journal of Dairy Research.

[178]  J. Goldberg,et al.  The phosphorylcholine epitope undergoes phase variation on a 43-kilodalton protein in Pseudomonas aeruginosa and on pili of Neisseria meningitidis and Neisseria gonorrhoeae. , 1998, Infection and immunity.

[179]  T. Ross,et al.  Variability in biofilm production by Listeria monocytogenes correlated to strain origin and growth conditions. , 2011, International journal of food microbiology.

[180]  Hinrich Hartmann,et al.  Anaerobic digestion of the organic fraction of municipal solid waste: influence of co-digestion with manure. , 2005, Water research.

[181]  M. Sugai,et al.  Clonal distribution of enterotoxigenic Staphylococcus aureus on handles of handheld shopping baskets in supermarkets , 2011, Journal of applied microbiology.

[182]  R. Donlan,et al.  Biofilms: Microbial Life on Surfaces , 2002, Emerging infectious diseases.