A Systematic Workflow for Design and Computational Analysis of Protein Microarrays

High-density protein microarrays allow to display a high number of analytes using minimal amounts of samples, constituting a promising high-throughput platform for the characterization of protein expression patterns, functional analysis and biomarker discovery. According to main characteristics, many different types of protein microarrays have been described and applied in several biomedical research projects. In all of these cases, the analysis is a critical step which has to be optimized (occasionally customized) depending the protein array type, assay features, array and experimental design. Here, it is proposed a general workflow for systematic and comprehensive data analysis of protein microarrays.

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