Differentiating atherosclerotic plaque burden in arterial tissues using femtosecond CARS-based multimodal nonlinear optical imaging

A femtosecond CARS-based nonlinear optical microscope was used to simultaneously image extracellular structural proteins and lipid-rich structures within intact aortic tissue obtained from myocardial infarction-prone Watanabe heritable hyperlipidemic rabbits (WHHLMI). Clear differences in the NLO microscopic images were observed between healthy arterial tissue and regions dominated by atherosclerotic lesions. In the current ex-vivo study, we present a single parameter based on intensity changes derived from multi-channel NLO image to classify plaque burden within the vessel. Using this parameter we were able to differentiate between healthy regions of the vessel and regions with plaque, as well as distinguish plaques relative to the age of the WHHLMI rabbit.

[1]  Karel Svoboda,et al.  ScanImage: Flexible software for operating laser scanning microscopes , 2003, Biomedical engineering online.

[2]  Conor L Evans,et al.  Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[3]  A F van der Steen,et al.  Intravascular ultrasound combined with Raman spectroscopy to localize and quantify cholesterol and calcium salts in atherosclerotic coronary arteries. , 2000, Arteriosclerosis, thrombosis, and vascular biology.

[4]  Woonggyu Jung,et al.  Design and implementation of fiber-based multiphoton endoscopy with microelectromechanical systems scanning. , 2009, Journal of biomedical optics.

[5]  Abigail S Haka,et al.  In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque. , 2006, Journal of biomedical optics.

[6]  Hung-Che Chen,et al.  Quantitative assessment of hepatic fat of intact liver tissues with coherent anti-stokes Raman scattering microscopy. , 2009, Analytical chemistry.

[7]  Aldons J. Lusis,et al.  Atherosclerosis : Vascular biology , 2000 .

[8]  Brett E. Bouma,et al.  In Vivo Characterization of Coronary Atherosclerotic Plaque by Use of Optical Coherence Tomography , 2005, Circulation.

[9]  Andrew Ridsdale,et al.  Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction-prone rabbits. , 2010, Journal of biomedical optics.

[10]  B. Lewis,et al.  Hereditary Hyperlipidemia and Atherosclerosis in the Rabbit Due to Overproduction of Lipoproteins: II. Preliminary Report of Arterial Pathology , 1987, Arteriosclerosis.

[11]  J. Hodgson,et al.  Intracoronary ultrasound imaging: correlation of plaque morphology with angiography, clinical syndrome and procedural results in patients undergoing coronary angioplasty. , 1993, Journal of the American College of Cardiology.

[12]  R. Kloner,et al.  Quantitative assessment of myocardial collagen with picrosirius red staining and circularly polarized light , 1994, Basic Research in Cardiology.

[13]  P. Fitzgerald,et al.  Intravascular ultrasound: state of the art and future directions. , 1998, The American journal of cardiology.

[14]  Ji-Xin Cheng,et al.  Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy. , 2007, Journal of biomedical optics.

[15]  Lingfeng Yu,et al.  Rotational multiphoton endoscopy with a 1 μm fiber laser system , 2009 .

[16]  W. Denk,et al.  Deep tissue two-photon microscopy , 2005, Nature Methods.

[17]  W. Webb,et al.  Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[18]  Yujie Zhou,et al.  Identification and quantification of coronary atherosclerotic plaques: a comparison of 64-MDCT and intravascular ultrasound. , 2008, AJR. American journal of roentgenology.

[19]  Hideo Tashiro,et al.  Intravascular Raman spectroscopic catheter for molecular diagnosis of atherosclerotic coronary disease. , 2006, Applied optics.

[20]  Joseph M Schmitt,et al.  Current and future developments in intracoronary optical coherence tomography imaging. , 2009, EuroIntervention : journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology.

[21]  Watt W Webb,et al.  Interpreting second-harmonic generation images of collagen I fibrils. , 2005, Biophysical journal.

[22]  Leslie M Loew,et al.  Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms , 2003, Nature Biotechnology.

[23]  Jianglin Fan,et al.  Development of an animal model for spontaneous myocardial infarction (WHHLMI rabbit). , 2003, Arteriosclerosis, thrombosis, and vascular biology.

[24]  Yasuhiro Honda,et al.  Frontiers in intravascular imaging technologies. , 2008, Circulation.

[25]  B. Tromberg,et al.  Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[26]  P. Fitzgerald,et al.  'Optimal' directional coronary atherectomy: final results of the Optimal Atherectomy Restenosis Study (OARS). , 1998, Circulation.

[27]  Graham A Wright,et al.  Innovations in imaging for chronic total occlusions: a glimpse into the future of angiography's blind-spot. , 2008, European heart journal.

[28]  W D Wagner,et al.  A definition of advanced types of atherosclerotic lesions and a histological classification of atherosclerosis. A report from the Committee on Vascular Lesions of the Council on Arteriosclerosis, American Heart Association. , 1995, Arteriosclerosis, thrombosis, and vascular biology.

[29]  W. Webb,et al.  Nonlinear magic: multiphoton microscopy in the biosciences , 2003, Nature Biotechnology.

[30]  P. Serruys,et al.  Optical coherence tomography assessment of vulnerable plaque rupture: predilection for the plaque 'shoulder'. , 2008, European heart journal.

[31]  T. Kita,et al.  Cellular Pathology of Progressive Atherosclerosis in the WHHL Rabbit: An Animal Model of Familial Hypercholesterolemia , 1983, Arteriosclerosis.

[32]  Lingfeng Yu,et al.  Rotational multiphoton endoscopy with a 1 microm fiber laser system. , 2009, Optics Letters.

[33]  Bruce J Tromberg,et al.  Imaging coronary artery microstructure using second-harmonic and two-photon fluorescence microscopy. , 2004, Biophysical journal.

[34]  Landulfo Silveira,et al.  Raman spectroscopy study of atherosclerosis in human carotid artery. , 2005, Journal of biomedical optics.

[35]  X. Xie,et al.  Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy Published, JLR Papers in Press, August 16, 2003. DOI 10.1194/jlr.D300022-JLR200 , 2003, Journal of Lipid Research.

[36]  Gangjun Liu,et al.  Fiber delivered probe for efficient CARS imaging of tissues. , 2010, Optics express.

[37]  Peter Libby,et al.  Atherosclerosis: disease biology affecting the coronary vasculature. , 2006, The American journal of cardiology.

[38]  T. T. Le,et al.  Label-free Imaging of Arterial Cells and Extracellular Matrix Using a Multimodal CARS Microscope. , 2008, Optics communications.

[39]  Andrew Ridsdale,et al.  Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator. , 2009, Optics express.

[40]  Jianglin Fan,et al.  Correlation of vulnerable coronary plaques to sudden cardiac events. Lessons from a myocardial infarction-prone animal model (the WHHLMI rabbit). , 2004, Journal of atherosclerosis and thrombosis.

[41]  Ji-Xin Cheng,et al.  Imaging and Quantitative Analysis of Atherosclerotic Lesions by CARS-Based Multimodal Nonlinear Optical Microscopy , 2009, Arteriosclerosis, thrombosis, and vascular biology.

[42]  Aldo Brugnera,et al.  Use of near-infrared Raman spectroscopy for identification of atherosclerotic plaques in the carotid artery. , 2007, Photomedicine and laser surgery.

[43]  Catharina de Lange Davies,et al.  Characterization of vulnerable plaques by multiphoton microscopy. , 2007, Journal of biomedical optics.

[44]  A. Pena,et al.  Second harmonic imaging and scoring of collagen in fibrotic tissues. , 2007, Optics express.

[45]  X. Xie,et al.  Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications , 2004 .