Mediator facilitates transcriptional activation and dynamic long-range contacts at the IgH locus during class switch recombination

Thomas-Claudepierre et al. report that mediator facilitates the long-range contacts between acceptor switch regions and the IgH locus enhancers during class switch recombination and their transcriptional activation.

[1]  Dr. P. K. Chaudhary.  Accepted , 2018, Definitions.

[2]  Alexander J. Federation,et al.  RNA Exosome-Regulated Long Non-Coding RNA Transcription Controls Super-Enhancer Activity , 2015, Cell.

[3]  A. Kenter,et al.  Constraints Contributed by Chromatin Looping Limit Recombination Targeting during Ig Class Switch Recombination , 2015, The Journal of Immunology.

[4]  Dylan J. Taatjes,et al.  The Mediator complex: a central integrator of transcription , 2015, Nature Reviews Molecular Cell Biology.

[5]  Yijun Ruan,et al.  B Cell Super-Enhancers and Regulatory Clusters Recruit AID Tumorigenic Activity , 2014, Cell.

[6]  A. Feeney,et al.  Targeted Chromatin Profiling Reveals Novel Enhancers in Ig H and Ig L Chain Loci , 2014, The Journal of Immunology.

[7]  M. Nussenzweig,et al.  Epigenetic targeting of activation-induced cytidine deaminase , 2014, Proceedings of the National Academy of Sciences.

[8]  B. Reina-San-Martin,et al.  The cohesin complex regulates immunoglobulin class switch recombination , 2013, The Journal of experimental medicine.

[9]  F. Alt,et al.  Flexible long-range loops in the VH gene region of the Igh locus facilitate the generation of a diverse antibody repertoire. , 2013, Immunity.

[10]  Jennifer E. Phillips-Cremins,et al.  Architectural Protein Subclasses Shape 3D Organization of Genomes during Lineage Commitment , 2013, Cell.

[11]  David A. Orlando,et al.  Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes , 2013, Cell.

[12]  Yuval Kluger,et al.  Close proximity to Igh is a contributing factor to AID-mediated translocations. , 2012, Molecular cell.

[13]  Scott Feldman,et al.  Three‐dimensional architecture of the IgH locus facilitates class switch recombination , 2012, Annals of the New York Academy of Sciences.

[14]  T. Borggrefe,et al.  Essential role of Mediator subunit Med1 in invariant natural killer T-cell development , 2011, Proceedings of the National Academy of Sciences.

[15]  R. Conaway,et al.  Origins and activity of the Mediator complex. , 2011, Seminars in cell & developmental biology.

[16]  T. Borggrefe,et al.  Interactions between subunits of the Mediator complex with gene-specific transcription factors. , 2011, Seminars in cell & developmental biology.

[17]  F. Alt,et al.  Epigenetic tethering of AID to the donor switch region during immunoglobulin class switch recombination , 2011, The Journal of experimental medicine.

[18]  F. Alt,et al.  The RNA Exosome Targets the AID Cytidine Deaminase to Both Strands of Transcribed Duplex DNA Substrates , 2011, Cell.

[19]  H. Luche,et al.  Specific erythroid-lineage defect in mice conditionally deficient for Mediator subunit Med1 , 2010, Proceedings of the National Academy of Sciences.

[20]  Sohail Malik,et al.  The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation , 2010, Nature Reviews Genetics.

[21]  David A. Orlando,et al.  Mediator and Cohesin Connect Gene Expression and Chromatin Architecture , 2010, Nature.

[22]  W. Huber,et al.  which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. MAnorm: a robust model for quantitative comparison of ChIP-Seq data sets , 2011 .

[23]  Clifford A. Meyer,et al.  Model-based Analysis of ChIP-Seq (MACS) , 2008, Genome Biology.

[24]  F. Alt,et al.  S-S synapsis during class switch recombination is promoted by distantly located transcriptional elements and activation-induced deaminase. , 2007, Immunity.

[25]  M. Reth,et al.  Testing gene function early in the B cell lineage in mb1-cre mice , 2006, Proceedings of the National Academy of Sciences.

[26]  P. Chambon,et al.  Transcription Coactivator PBP, the Peroxisome Proliferator-activated Receptor (PPAR)-binding Protein, Is Required for PPARα-regulated Gene Expression in Liver* , 2004, Journal of Biological Chemistry.

[27]  M. Neuberger,et al.  AID mutates E. coli suggesting a DNA deamination mechanism for antibody diversification , 2002, Nature.

[28]  J. Reddy,et al.  Deletion of PBP/PPARBP, the Gene for Nuclear Receptor Coactivator Peroxisome Proliferator-activated Receptor-binding Protein, Results in Embryonic Lethality* , 2000, The Journal of Biological Chemistry.

[29]  R. Darnell,et al.  Involvement of the TRAP220 component of the TRAP/SMCC coactivator complex in embryonic development and thyroid hormone action. , 2000, Molecular cell.

[30]  A. Kenter,et al.  Constraints Contributed by Chromatin Looping Limit Recombination Targeting during Immunoglobulin Class Switch Recombination , 2015 .

[31]  G. Marit,et al.  Primers and probes used in this study. , 2015 .

[32]  M. Nussenzweig,et al.  AID targeting in antibody diversity. , 2011, Advances in immunology.

[33]  M. Nussenzweig,et al.  Deep-sequencing identification of the genomic targets of the cytidine deaminase AID and its cofactor RPA in B lymphocytes , 2011, Nature Immunology.

[34]  F. Alt,et al.  Evolution of the immunoglobulin heavy chain class switch recombination mechanism. , 2007, Advances in immunology.