Manual Whisker Annotator (MWA): A Modular Open-Source Tool

Rodents are key to generating translational data for healthcare research. Behavioural analyses, in particular, are integral to the non-invasive monitoring of rodent health and welfare. Finding quantitative behavioural measures mitigates stress, allowing for the animal behave freely while also enabling the same animal to be studied over the time-course of its life. Locomotion and whisking are both such quantitative behavioural measures, and have been found to be significantly impacted in rodent models of neurodegenerative disease. While automatic trackers of whiskers and locomotion exist, a manual tracker is required to validate these approaches, and also to annotate complex videos where these automatic versions fail. Manually annotating whiskers for research purposes is a long and tedious task and current software does little to provide an intuitive and simple interface to carry out this task. This led to the creation of the Manual Whisker Annotator (MWA). MWA is an open source, portable whisker annotation tool developed for the Windows platform. Not only does MWA make the process much quicker, it also provides added statistical tools to analyse the data. MWA was developed in C# and WPF using the .NET framework, and could be used in any situation where annotating or tracking multiple targets is desired.

[1]  Karel Svoboda,et al.  Natural Whisker-Guided Behavior by Head-Fixed Mice in Tactile Virtual Reality , 2014, The Journal of Neuroscience.

[2]  Moi Hoon Yap,et al.  Formulating efficient software solution for digital image processing system , 2016, Softw. Pract. Exp..

[3]  H. Philip Zeigler,et al.  Whisker Deafferentation and Rodent Whisking Patterns: Behavioral Evidence for a Central Pattern Generator , 2001, The Journal of Neuroscience.

[4]  M. Brecht,et al.  Functional architecture of the mystacial vibrissae , 1997, Behavioural Brain Research.

[5]  Per Magne Knutsen,et al.  Object localization with whiskers , 2008, Biological Cybernetics.

[6]  Tony J. Prescott,et al.  Strategy Change in Vibrissal Active Sensing during Rat Locomotion , 2014, Current Biology.

[7]  Dario Campagner,et al.  Microsecond-Scale Timing Precision in Rodent Trigeminal Primary Afferents , 2015, The Journal of Neuroscience.

[8]  Dori Derdikman,et al.  Tracking whisker and head movements in unrestrained behaving rodents. , 2005, Journal of neurophysiology.

[9]  S. B. Vincent.  The function of the vibrissae in the behavior of the white rat , 1912 .

[10]  F. Harris.  On the use of windows for harmonic analysis with the discrete Fourier transform , 1978, Proceedings of the IEEE.

[11]  Anthony G. Pipe,et al.  Whiskerbot: A Robotic Active Touch System Modeled on the Rat Whisker Sensory System , 2007, Adapt. Behav..

[12]  Andrej Kosir,et al.  Unsupervised quantification of whisking and head movement in freely moving rodents. , 2011, Journal of neurophysiology.

[13]  T. Woolsey,et al.  The structural organization of layer IV in the somatosensory region (SI) of mouse cerebral cortex. The description of a cortical field composed of discrete cytoarchitectonic units. , 1970, Brain research.

[14]  Eugene W. Myers,et al.  Automated Tracking of Whiskers in Videos of Head Fixed Rodents , 2012, PLoS Comput. Biol..

[15]  M. Hartmann,et al.  Mechanical Characteristics of Rat Vibrissae: Resonant Frequencies and Damping in Isolated Whiskers and in the Awake Behaving Animal , 2003, The Journal of Neuroscience.

[16]  T. Prescott,et al.  The role of orienting in vibrissal touch sensing , 2012, Front. Behav. Neurosci..

[17]  Mathew H. Evans,et al.  Prediction of primary somatosensory neuron activity during active tactile exploration , 2015, bioRxiv.

[18]  E. Ahissar,et al.  Encoding of Vibrissal Active Touch , 2003, Neuron.

[19]  T. Prescott,et al.  Active touch sensing in the rat: anticipatory and regulatory control of whisker movements during surface exploration. , 2009, Journal of neurophysiology.

[20]  Ben Mitchinson,et al.  Feedback control in active sensing: rat exploratory whisking is modulated by environmental contact , 2007, Proceedings of the Royal Society B: Biological Sciences.

[21]  G. Farin.  Algorithms for rational Bézier curves , 1983 .

[22]  Nathan G. Clack,et al.  The Mechanical Variables Underlying Object Localization along the Axis of the Whisker , 2013, The Journal of Neuroscience.

[23]  Gary R. Bradski,et al.  Learning OpenCV 3: Computer Vision in C++ with the OpenCV Library , 2016 .

[24]  Tony J. Prescott,et al.  Abnormalities in whisking behaviour are associated with lesions in brain stem nuclei in a mouse model of amyotrophic lateral sclerosis , 2014, Behavioural Brain Research.

[25]  T. Prescott,et al.  The development of whisker control in rats in relation to locomotion. , 2012, Developmental psychobiology.

[26]  D. Simons,et al.  Biometric analyses of vibrissal tactile discrimination in the rat , 1990, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[27]  David Kleinfeld.  Vibrissa Movement, Sensation and Sensorimotor Control , 2009 .

[28]  Rune W. Berg,et al.  Rhythmic whisking by rat: retraction as well as protraction of the vibrissae is under active muscular control. , 2003, Journal of neurophysiology.

[29]  M. Hartmann,et al.  Right–Left Asymmetries in the Whisking Behavior of Rats Anticipate Head Movements , 2006, The Journal of Neuroscience.

[30]  T. Woolsey,et al.  The structural organization of layer IV in the somatosensory region (S I) of mouse cerebral cortex , 1970 .