Cholinergic and monoaminergic innervation of the cat's thalamus: Comparison of the lateral geniculate nucleus with other principal sensory nuclei

The cholinergic and monoaminergic innervation of the lateral geniculate nucleus (GL) and other thalamic nuclei in the cat was examined by using immunocytochemical and tract‐tracing techniques. Cholinergic fibers, identified with an antibody to choline acetyltransferase (ChAT), are present in all layers of the GL. They are fine in caliber and exhibit numerous swellings along their lengths. The A layers, the magnocellular C layer, and the medial interlaminar nucleus are rich in cholinergic fibers that give rise to prominent clusters of boutons, while the parvicellular C layers contain fewer fibers that are more uniformly distributed. The interlaminar zones are largely devoid of ChAT‐immunoreactive fibers. Double‐label experiments show that cholinergic projections to the GL originate from two sources, the pedunculopontine reticular formation (PPT) and the parabigeminal nucleus (Pbg). The PPT contributes cholinergic fibers to all layers, while Pbg projections are limited to the parvicellular C layers.