In vivo reproducibility of three‐dimensional structural properties of noninvasive bone biopsies using 3D‐pQCT

Trabecular bone architecture is one of the main factors influencing the mechanical behavior of cancellous bone. To assess the three‐dimensional trabecular microstructure of intact bones, we introduced the concept of noninvasive bone biopsy, a method to assess and analyze cancellous bone based upon three‐dimensional peripheral quantitative computed tomography in vivo (3D‐pQCT). The aim of this work was to demonstrate the potential of noninvasive bone biopsies as a basis for structural and mechanical analysis of trabecular bone in the process of rapid bone loss. A group of six healthy young male volunteers was measured to provide data on the reproducibility of structural parameters. Baseline and 1‐month follow‐up measurements were performed to provide data on short‐term precision of the procedure, and three of the controls were reanalyzed within 3–6 months to estimate long‐term precision. Prior to structural evaluation, the baseline and follow‐up measurements were repositioned three‐dimensionally to ensure matching volumes of interest (VOI). Trabecular bone density (TBD) as well as structural indices were analyzed for all measurements. The VOIs were analyzed morphometrically by evaluating bone volume (BV/TV) and trabecular number (Tb.N) based on a direct three‐dimensional approach. Trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) were derived from these two indices. The data of the measurements at 1 month to determine the short‐term precision was in excellent agreement with the baseline measurements. The results showed that structural parameters can be reproduced in vivo with a coefficient of variation of less than 0.5%. With a typical spread of 4% for the structural indices within the group of healthy volunteers, an intraclass correlation of better than 0.98 was reached. We conclude that high‐resolution 3D‐pQCT has the potential to detect structural changes in trabecular bone during therapeutic and diagnostic trials. (J Bone Miner Res 1996;11:1745‐1750)

[1]  P Rüegsegger,et al.  Analysis of mechanical properties of cancellous bone under conditions of simulated bone atrophy. , 1996, Journal of biomechanics.

[2]  P. Rüegsegger,et al.  Morphometric analysis of noninvasively assessed bone biopsies: comparison of high-resolution computed tomography and histologic sections. , 1996, Bone.

[3]  S. Majumdar,et al.  Evaluation of technical factors affecting the quantification of trabecular bone structure using magnetic resonance imaging. , 1995, Bone.

[4]  J. Kinney,et al.  In vivo, three‐dimensional microscopy of trabecular bone , 1995, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[5]  Peter Rüegsegger,et al.  The use of peripheral QCT in the evaluation of bone remodelling , 1994 .

[6]  S A Goldstein,et al.  The relationship between the structural and orthogonal compressive properties of trabecular bone. , 1994, Journal of biomechanics.

[7]  M. Hahn,et al.  High Spatial Resolution Imaging of Bone Mineral Using Computed Microtomography: Comparison with Microradiography and Undecalcified Histologic Sections , 1993, Investigative radiology.

[8]  F. Wehrli,et al.  High‐resolution variable flip angle 3D MR imaging of trabecular microstructure in vivo , 1993, Magnetic resonance in medicine.

[9]  M Vogel,et al.  Trabecular bone pattern factor--a new parameter for simple quantification of bone microarchitecture. , 1992, Bone.

[10]  F Melsen,et al.  A direct method for fast three‐dimensional serial reconstruction , 1990, Journal of microscopy.

[11]  S. Goldstein,et al.  The direct examination of three‐dimensional bone architecture in vitro by computed tomography , 1989, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[12]  P. Meunier,et al.  Age-related changes in cancellous bone structure. A two-dimensional study in the transiliac and iliac crest biopsy sites. , 1988, Bone and mineral.

[13]  R. Mann,et al.  Characterization of microstructural anisotropy in orthotropic materials using a second rank tensor , 1984 .

[14]  P Rüegsegger,et al.  Non-invasive bone biopsy: a new method to analyse and display the three-dimensional structure of trabecular bone. , 1994, Physics in medicine and biology.

[15]  B. Munch,et al.  3-D repositioning and differential images of volumetric CT measurements. , 1993, IEEE transactions on medical imaging.

[16]  Noboru Kikuchi,et al.  The Mechanical and Remodeling Properties of Trabecular Bone , 1990 .

[17]  L. Mosekilde.  Age-related changes in vertebral trabecular bone architecture--assessed by a new method. , 1988, Bone.

[18]  J. Compston,et al.  Age-related changes in iliac crest trabecular microanatomic bone structure in man. , 1987, Bone.