Effects of High Sludge Cycle Frequency on Performance and Syntrophic Metabolism of Anaerobic Membrane Bioreactor for Treating High-Lipid Kitchen Waste Slurry

The performance and syntrophic metabolism of the Anaerobic Membrane Bio-reactor (AnMBR) treating high-lipid kitchen waste slurry under different sludge cycle frequencies were investigated in this study. When the sludge cycle frequencies were 3.6 cycles/h, 9.0 cycles/h and 14.4 cycles/h, the obtained Organic Loading Rates (OLRs) were 10.3 kg-COD/m3d, 12.4 kg-COD/m3d and 18.1 kg-COD/m3d, while the corresponding biogas productions were 190 L/d, 310 L/d and 520 L/d. Moreover, with an increase of sludge cycle frequency, the Chemical Oxygen Demand (COD) removal efficiency improved from 86.2% to 90.4% and 96.3%. Additionally, the higher sludge cycle frequency did not break up the sludge flocs and further affect the syntrophic degradation of the toxic Long-Chain Fatty Acids (LCFAs). Conversely, the higher sludge cycle frequency enhanced LCFA degradation and decreased LCFA accumulation. Meanwhile, under higher sludge cycle frequencies, the abundance of syntrophic Methanobacterium, Syntrophomonas and Clostridium increased and favored the syntrophic metabolism of LCFAs.

[1]  M. Alves,et al.  Accumulation of long chain fatty acids onto anaerobic sludge under steady state and shock loading conditions: effect on acetogenic and methanogenic activity. , 2003, Water science and technology : a journal of the International Association on Water Pollution Research.

[2]  C. Forster,et al.  Continuous co-digestion of cattle slurry with fruit and vegetable wastes and chicken manure , 2002 .

[3]  A. Stams,et al.  Molecular assessment of complex microbial communities degrading long chain fatty acids in methanogenic bioreactors. , 2007, FEMS microbiology ecology.

[4]  Freda R. Hawkes,et al.  Comparative performance of anaerobic digesters operating on ice-cream wastewater , 1995 .

[5]  Wenquan Ruan,et al.  Evaluation and characterization during the anaerobic digestion of high-strength kitchen waste slurry via a pilot-scale anaerobic membrane bioreactor. , 2015, Bioresource technology.

[6]  Jan A. Oleszkiewicz,et al.  Anaerobic co‐composting of municipal solid waste and waste sludge at hlgh total solids levels , 1992 .

[7]  M. Holtzapple,et al.  Fermentation of corn stover to carboxylic acids. , 2003, Biotechnology and bioengineering.

[8]  Hauke Smidt,et al.  Syntrophomonas zehnderi sp. nov., an anaerobe that degrades long-chain fatty acids in co-culture with Methanobacterium formicicum. , 2007, International journal of systematic and evolutionary microbiology.

[9]  Cheng-nan Weng,et al.  Biochemical mechanisms in the methane fermentation of glutamic and oleic acids , 1976 .

[10]  Guang-qing Liu,et al.  Characterization of food waste as feedstock for anaerobic digestion. , 2007, Bioresource technology.

[11]  N. Zhu,et al.  Role of extracellular protein in the formation and stability of aerobic granules , 2007 .

[12]  F. Smith,et al.  Colorimetric Method for Determination of Sugars and Related Substances , 1956 .

[13]  N. Abdurahman,et al.  Development of a membrane anaerobic system (MAS) for palm oil mill effluent (POME) treatment , 2011 .

[14]  Gatze Lettinga,et al.  Acute toxicity of oleate to acetate-utilizing methanogens in mesophilic and thermophilic anaerobic sludges , 1997 .

[15]  Hengfeng Miao,et al.  Enhancing biogas generation performance from food wastes by high-solids thermophilic anaerobic digestion: Effect of pH adjustment , 2015 .

[16]  Haochen Zhu,et al.  The Content Variation of Fat, Protein and Starch in Kitchen Waste Under Microwave Radiation , 2016 .

[17]  Hyun-Ju Park,et al.  Approaches to membrane fouling control in anaerobic membrane bioreactors , 2000 .

[18]  Xiaolan Xiao,et al.  Impacts of sludge retention time on the performance of submerged membrane bioreactor with the addition of calcium ion , 2011 .

[19]  Control of membrane fouling during hyperhaline municipal wastewater treatment using a pilot-scale anoxic/aerobic-membrane bioreactor system. , 2011, Journal of environmental sciences.

[20]  Wenquan Ruan,et al.  Enzymatic dynamics of microbial acid tolerance response (ATR) during the enhanced biohydrogen production process via anaerobic digestion , 2012 .

[21]  K. Keiding,et al.  Extraction of extracellular polymers from activated sludge using a cation exchange resin , 1996 .

[22]  O. C. Pires,et al.  Anaerobic biodegradation of oleic and palmitic acids: evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge. , 2005, Biotechnology and bioengineering.

[23]  D. G. Cirnea,et al.  Anaerobic digestion of lipid-rich waste — Effects of lipid concentration , 2006 .

[24]  B. Mattiasson,et al.  Anaerobic digestion of lipid-rich waste - Effects of lipid concentration , 2007 .

[25]  Jacques Ravel,et al.  An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform , 2014, Microbiome.

[26]  Jolanta Bohdziewicz,et al.  Landfill leachate treatment by means of anaerobic membrane bioreactor , 2008 .

[27]  Guang-qing Liu,et al.  Anaerobic digestion of lipid-rich swine slaughterhouse waste: Methane production performance, long-chain fatty acids profile and predominant microorganisms. , 2018, Bioresource technology.

[28]  M. Alves,et al.  Detection and quantification of long chain fatty acids in liquid and solid samples and its relevance to understand anaerobic digestion of lipids. , 2009, Bioresource technology.

[29]  B. Lewa,et al.  Anaerobic membrane bioreactor ( AnMBR ) for domestic wastewater treatment , 2009 .

[30]  H Siegrist,et al.  Anaerobic digestion of slaughterhouse waste with UF-membrane separation and recycling of permeate after free ammonia stripping. , 2005, Water science and technology : a journal of the International Association on Water Pollution Research.

[31]  C. F. Seyfried,et al.  Sludge activity and cross-flow microfiltration - a non-beneficial relationship , 1996 .

[32]  A. Rinzema,et al.  Bactericidal effect of long chain fatty acids in anaerobic digestion , 1994 .

[33]  B. Schink.  Energetics of syntrophic cooperation in methanogenic degradation , 1997, Microbiology and molecular biology reviews : MMBR.

[34]  Zhengyu Jin,et al.  Membrane fouling controlled by coagulation/adsorption during direct sewage membrane filtration (DSMF) for organic matter concentration. , 2015, Journal of environmental sciences.

[35]  Quevedo Amaya,et al.  Caracterización fisiológica y bioquímica de cuatro genotipos de algodón (Gossypium hirsutum L.) en condiciones de déficit hídrico , 2020 .

[36]  A. Stams,et al.  Interspecies electron transfer in methanogenic propionate degrading consortia. , 2004, Water research.

[37]  Y Y Li,et al.  High-rate methane fermentation of lipid-rich food wastes by a high-solids co-digestion process. , 2002, Water science and technology : a journal of the International Association on Water Pollution Research.

[38]  R. Dereli,et al.  Effect of sludge retention time on the biological performance of anaerobic membrane bioreactors treating corn-to-ethanol thin stillage with high lipid content. , 2014, Water research.

[39]  I. Koster,et al.  Inhibition of Methanogenesis from Acetate in Granular Sludge by Long-Chain Fatty Acids , 1987, Applied and environmental microbiology.

[40]  Keisuke Hanaki,et al.  Mechanism of inhibition caused by long‐chain fatty acids in anaerobic digestion process , 1981 .

[41]  Wenquan Ruan,et al.  Process stability and microbial response of anaerobic membrane bioreactor treating high-strength kitchen waste slurry under different organic loading rates , 2017 .