Cloning and characterization of the mouse vitamin D receptor promoter.

The gene encoding the mouse vitamin D receptor has been cloned. A new exon 1 has been found that changes the numbering established for the human VDR gene. Exons 2 and 3 in the human VDR gene (coding for the zinc fingers 1 and 2, respectively) are named exons 3 and 4 in the mouse vitamin D receptor. The 1.5-kb 5'-flanking region of the new exon 1 was analyzed and revealed the presence of putative cis-acting elements. Despite the absence of a TATA box, this 5'-flanking region contains several characteristics of a GC-rich promoter including four Sp1 sites present in tandem and two CCAAT boxes. Interestingly, the Sp1 site that is the most proximal to the new exon 1 overlaps a perfect site for Krox-20/24. Krox-20 is a transcription factor involved in brain development, and also in bone remodeling. In luciferase reporter gene expression assays, we showed that sequences from this 5'-flanking region elicit high transactivation activity. Furthermore, in the NIH 3T3 cell line, a 3- to 5-fold increase in response to forskolin treatment (an activator of adenylate cyclase and in turn of protein kinase A pathway) was observed.

[1]  M. Demay,et al.  Cloning and Characterization of the Vitamin D Receptor from Xenopus laevis. , 1997, Endocrinology.

[2]  H. DeLuca,et al.  Cloning and origin of the two forms of chicken vitamin D receptor. , 1997, Archives of biochemistry and biophysics.

[3]  G. Karsenty,et al.  A PEBP2/AML-1-related Factor Increases Osteocalcin Promoter Activity through Its Binding to an Osteoblast-specific cis-Acting Element (*) , 1995, The Journal of Biological Chemistry.

[4]  G. Stein,et al.  The tissue-specific nuclear matrix protein, NMP-2, is a member of the AML/CBF/PEBP2/runt domain transcription factor family: interactions with the osteocalcin gene promoter. , 1995, Biochemistry.

[5]  H. DeLuca,et al.  Identification of the porcine intestinal accessory factor that enables DNA sequence recognition by vitamin D receptor. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[6]  H. DeLuca,et al.  The avian vitamin D receptors: primary structures and their origins. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[7]  S. Schneider-Maunoury,et al.  Krox-20 controls myelination in the peripheral nervous system , 1994, Nature.

[8]  S. Schneider-Maunoury,et al.  Disruption of Krox-20 results in alteration of rhombomeres 3 and 5 in the developing hindbrain , 1993, Cell.

[9]  H. DeLuca,et al.  Stabilization of the vitamin D receptor in rat osteosarcoma cells through the action of 1,25-dihydroxyvitamin D3. , 1993, Molecular endocrinology.

[10]  M. Galligan,et al.  Retinoid X receptors stimulate and 9-cis retinoic acid inhibits 1,25-dihydroxyvitamin D3-activated expression of the rat osteocalcin gene , 1993, Molecular and cellular biology.

[11]  H. DeLuca,et al.  The necessity for calcium for increased renal vitamin D receptor in response to 1,25-dihydroxyvitamin D. , 1993, Biochimica et biophysica acta.

[12]  H. DeLuca,et al.  Up-regulation of the vitamin D receptor in response to 1,25-dihydroxyvitamin D3 results from ligand-induced stabilization. , 1992, The Journal of biological chemistry.

[13]  H. Pols,et al.  Regulation of 1,25-dihydroxyvitamin D3 receptor gene expression by parathyroid hormone and cAMP-agonists. , 1992, Biochemical and biophysical research communications.

[14]  D. Feldman,et al.  Cyclic adenosine 3',5'-monophosphate up-regulates 1,25-dihydroxyvitamin D3 receptor gene expression and enhances hormone action. , 1992, Molecular endocrinology.

[15]  H. Stunnenberg,et al.  The serum-inducible mouse gene Krox-24 encodes a sequence-specific transcriptional activator , 1990, Molecular and cellular biology.

[16]  H. DeLuca,et al.  Serum calcium and vitamin D regulate 1,25-dihydroxyvitamin D3 receptor concentration in rat kidney in vivo. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[17]  K. Umesono,et al.  Jun-Fos and receptors for vitamins A and D recognize a common response element in the human osteocalcin gene , 1990, Cell.

[18]  M. Klemsz,et al.  The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene , 1990, Cell.

[19]  M. Vigneron,et al.  The segment‐specific gene Krox‐20 encodes a transcription factor with binding sites in the promoter region of the Hox‐1.4 gene. , 1990, The EMBO journal.

[20]  B. O’Malley,et al.  Point mutations in the human vitamin D receptor gene associated with hypocalcemic rickets. , 1988, Science.

[21]  H. DeLuca,et al.  Structure and regulation of the rat 1,25-dihydroxyvitamin D3 receptor. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[22]  T. Visser,et al.  Heterologous up-regulation of the 1,25-dihydroxyvitamin D3 receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells. , 1988, Biochemical and biophysical research communications.

[23]  P. Sassone-Corsi.  Cyclic AMP induction of early adenovirus promoters involves sequences required for E1A trans-activation. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[24]  J. Shine,et al.  Cloning and expression of full-length cDNA encoding human vitamin D receptor. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[25]  Young-Sun Lin,et al.  Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[26]  H. DeLuca.  The vitamin D story: a collaborative effort of basic science and clinical medicine 1 , 1988, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[27]  H. DeLuca,et al.  Isolation and expression of rat 1,25-dihydroxyvitamin D3 receptor cDNA. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[28]  Marvin B. Shapiro,et al.  RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. , 1987, Nucleic acids research.

[29]  L. Sivaraman,et al.  In vivo identification of multiple promoter domains of adenovirus EIIA‐late promoter. , 1987, The EMBO journal.

[30]  M. Haussler,et al.  Molecular cloning of complementary DNA encoding the avian receptor for vitamin D. , 1987, Science.

[31]  H. DeLuca,et al.  Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: interaction with distinct receptor domains. , 1986, Biochemistry.

[32]  D. Feldman,et al.  Homologous up-regulation of the 1,25 (OH)2 vitamin D3 receptor in rats. , 1986, Biochemical and biophysical research communications.

[33]  J. Heersche,et al.  Retinoic acid stimulates 1,25-dihydroxyvitamin D3 binding in rat osteosarcoma cells. , 1984, The Journal of biological chemistry.

[34]  M. Haussler,et al.  Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. Generation by a preparation enriched in a 64,000-dalton protein. , 1983, The Journal of biological chemistry.

[35]  M. Walters.  An estrogen-stimulated 1,25-dihydroxyvitamin D3 receptor in rat uterus. , 1981, Biochemical and biophysical research communications.

[36]  R. Cancedda,et al.  Defective bone formation in Krox-20 mutant mice. , 1996, Development.

[37]  T. Kawada,et al.  Cloning and sequencing of the gene encoding the mouse vitamin D receptor. , 1995, Gene.

[38]  H. DeLuca,et al.  Molecular biology of vitamin D action. , 1994, Vitamins and hormones.

[39]  H. DeLuca,et al.  Vitamin D-regulated gene expression. , 1993, Critical reviews in eukaryotic gene expression.

[40]  P. Chambon,et al.  Characterization of a functional promoter for the human retinoic acid receptor-alpha (hRAR-alpha). , 1990, Nucleic acids research.

[41]  Robert Tjian,et al.  Promoter-specific activation of RNA polymerase II transcription by Sp1 , 1986 .